Hyaluronic acid

Manufactured by Abcam

Sourced in United Kingdom, United States

Hyaluronic acid is a high-molecular-weight glycosaminoglycan found naturally in the extracellular matrix of various tissues. It plays a crucial role in maintaining the structural integrity and hydration of the extracellular matrix.

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Lab products found in correlation

Heparan sulfate, by Abcam (2 mentions) Laminin, by Abcam (1 mentions) Fibronectin, by Abcam (1 mentions) Elastin, by Abcam (1 mentions) Masson s trichrome staining kit, by Polysciences (1 mentions) Hyaluronic acid, by R&D Systems (1 mentions) Pristine graphite powder, by Merck Group (1 mentions) Palmitic acid, by Merck Group (1 mentions) Metformin, by Merck Group (1 mentions) Chondroitin sulfate, by Abcam (1 mentions) Igg isotype control, by Abcam (1 mentions) Alexa fluor 488 igg, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Sp5 confocal microscope, by Leica (1 mentions)

Close spelling variants of this product

Hyaluronic acid ELISA Kit

4 protocols using hyaluronic acid

Investigating Extracellular Matrix Changes

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Normal and acellular heart biopsies were fixed in 4% formaldehyde. The paraffin sections were cut at 5-μm thickness and stained with Masson's Trichrome staining kit (Polysciences, Inc., Warrington, PA) to see the effect of surfactants on collagen arrangement in the tissue. In addition, the sections were stained for elastin, fibronectin, laminin, hyaluronic acid, and heparan sulfate (Abcam, Cambridge, United Kingdom) with the technique of immunohistochemistry.

Methe K., Bäckdahl H., Johansson B.R., Nayakawde N., Dellgren G, & Sumitran-Holgersson S. (2014). An Alternative Approach to Decellularize Whole Porcine Heart. BioResearch Open Access, 3(6), 327-338.

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Quantification of Endothelial Glycocalyx Degradation

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Commercial enzyme-linked immunosorbant assays (ELISAs) for soluble syndecan-1, chondroitin sulfate, heparan sulfate and hyaluronic acid were performed to quantify levels of endothelial glycocalyx component degradations (Syndecan-1: Abcam, Cat. No. ab46506, Cambridge, MA; Heparan Sulfate: Biotang, Cat. No. HU8718, Lexington, MA; Chondroitin Sulfate: Biotang, Cat. No. HU8720, Lexington, MA; hyaluronic acid: R&D Systems, Cat. No. DHYAL0, Minneapolis, MN). Adrenaline and noradrenaline were measured in uniplicate in plasma by commercially available ELISA kits (2-CAT ELISA; Labor Diagnostica Nord GmbH & Co KG, Nordhorn, Germany; lower limit of detection [LLD]) 10 pg/mL (adrenaline; normal reference, < 100 pg/mL) and 50 pg/mL (noradrenaline; normal reference, < 600 pg/mL), respectively [16 (link)]. Frozen citrated plasma samples were used for all ELISA assays, except for measuring HA and catecholamines, which used frozen plasma stored in Ethylenediaminetetraacetic acid (EDTA). All other samples were run in duplicate. Samples that were over the detection range of the assay were diluted and rerun as needed.

Rahbar E., Cardenas J.C., Baimukanova G., Usadi B., Bruhn R., Pati S., Ostrowski S.R., Johansson P.I., Holcomb J.B, & Wade C.E. (2015). Endothelial glycocalyx shedding and vascular permeability in severely injured trauma patients. Journal of Translational Medicine, 13, 117.

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Graphite, Metformin, and Palmitic Acid Protocol

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Pristine graphite powder, Metformin, and Palmitic acid were purchased from Sigma. Hyaluronic acid (Abcam), adipic acid di-hydrazide (ADH) (SRL), and DCFDA (SRL) were purchased. All other chemicals and solvents used in this study were of analytical grade.

Sarkar K., Bank S., Chatterjee A., Dutta K., Das A., Chakraborty S., Paul N., Sarkar J., De S., Ghosh S., Acharyya K., Chattopadhyay D, & Das M. (2023). Hyaluronic acid-graphene oxide quantum dots nanoconjugate as dual purpose drug delivery and therapeutic agent in meta-inflammation. Journal of Nanobiotechnology, 21, 246.

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Immunofluorescent Analysis of Endothelial ECM

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Specimens were fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton-X; the specimens were then blocked in 0.1% bovine serum albumin. Antibodies against ZO-1 (1:150, Invitrogen, USA), heparan sulfate (1:150, Abcam, USA), hyaluronic acid (1:150, Abcam, USA), and chondroitin sulfate (1:150, Abcam, USA), or IgG isotype control (1:150, Abcam, USA, Supplementary Figure 1) were incubated with the specimens overnight at 4°C. The specimens were then washed with PBS and incubated with Alexa Fluor 488 IgG (Invitrogen, USA) at 37°C for 1 h. Nuclei were stained with DAPI (1:100, Sigma, USA) at 37°C for 20 min. Finally, all samples were imaged with a Leica SP5 confocal microscope (Leica microsystem, Germany). The mean optical density was measured to reflect the content of ZO-1, heparan sulfate, hyaluronic acid, and chondroitin sulfate in endothelial cells.

Zhao H., Zhu Y., Zhang J., Wu Y., Xiang X., Zhang Z., Li T, & Liu L. (2020). The Beneficial Effect of HES on Vascular Permeability and Its Relationship With Endothelial Glycocalyx and Intercellular Junction After Hemorrhagic Shock. Frontiers in Pharmacology, 11, 597.

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