Odyssey ir imaging technology

Cells were lysed in UTB (9 M urea, 75 mM Tris-HCl pH 7.5, 0.15 M β-mercaptoethanol) and briefly sonicated. Primary antibodies used: HIF-1a (BD Transduction Labs., 610959), KDM4A (Abcam, ab24545), CAIX (BioScience, AB1001), Glut1 (Abcam, ab14683), Actin (Santa Cruz, sc-69879), H3K9me3 (Millipore, 07-422), H3K36me3 (Abcam, ab9050), H3 (Cell Signaling, 36385), NFκB p52 (Millipore, 05-361), Sp1 (Millipore, 07-645), E2F-1 (Cell Signaling, 3742S), HIF-2a (Novus Biologicals, NB100-122). Secondary antibodies were IRDye® 680RD Goat anti-Mouse IgG (H+L), IRDye® 680RD Goat anti-Rabbit IgG (H+L), IRDye® 800CW Donkey anti-Mouse IgG (H+L) and IRDye® 800CW Donkey anti-Rabbit IgG (H+L) from LI-COR Biosciences. Odyssey IR imaging technology (LI-COR Biosciences) was used for imaging.

Cells were collected and lysed in UTB (9 M urea, 75 mM Tris-HCl pH 7.5, 0.15 M β-mercaptoethanol) and briefly sonicated. Primary antibodies were HIF-1α (NB100-122 Novus Biologicals), H3K9Ac (9649S Cell Signaling), H3K18Ac (13998S Cell Signaling), H3K56Ac (4243S Cell Signaling), H3 total (3638S Cell Signaling), PARP (9542S Cell Signaling), GLUT1 (ab652 Abcam), α-tubulin (sc-5286 Santa Cruz), α-tubulin K40Ac (5335 Cell Signaling) and β−actin (sc-69876 Santa-Cruz biotechnology). Secondary antibodies were IRDye® 680RD Goat anti-Mouse IgG (H+L) and IRDye® 800CW Donkey anti-Rabbit IgG (H+L) from LI-COR Biosciences. Odyssey IR imaging technology (LI-COR Biosciences) was used for imaging.

Cells were collected and lysed in UTB (9 M urea, 75 mM Tris–HCl pH 7.5, 0.15 M β-mercaptoethanol) and briefly sonicated. Primary antibodies were LMP2 (ab3328, abcam), LMP7 (13635S, Cell Signaling), TAP2 (PA5-37414, Thermo Fisher), and β-actin (sc-69876 Santa-Cruz biotechnology). Secondary antibodies were IRDye680RD Goat anti-Mouse IgG (H + L) and IRDye 800CW Donkey anti-Rabbit IgG (H + L) from LI-COR Biosciences. Odyssey IR imaging technology (LI-COR Biosciences) was used for imaging.