Thermo lcq deca xp

The identification of bands on SDS‒PAGE gels was performed by LC‒MS/MS using a modified method as described in a previous paper [12 (link)]. Briefly, differentiated bands were cut out and digested in gel with trypsin overnight. After digestion, peptides were extracted and analyzed using an LC‒MS/MS system consisting of a Chorur 220 Nano pump, PAL autosampler, nano spray, and Thermo LCQ DECA XP. The Thermo LCQ DECA XP was equipped with an LC of instrument in gradient elution mode. The gradient ran from 5% acetonitrile with 0.1% formic acid at 0 min to 95% acetonitrile with 0.1% formic acid at 20 min. The Thermo LCQ DECA XP detected MS/MS spectra via a trap mass spectrometer. The obtained MS/MS spectra were used in a search of a database based on the scoring probability using the search algorithm SEQUEST. The database was composed of peptides from whole influenza virus proteins of respective CVVs and fragments of chicken proteins and human proteins from EMBL-EBI data resources and tools (https://www.ebi.ac.uk). Chicken proteins such as actin and human proteins such as keratin were used as peptides that could have been accidentally carried over from eggs and by contamination from workers.