Klh imject maleimide activated mcklh

Stock solutions of keyhole limpet hemocyanin (KLH-Imject Maleimide-Activated mcKLH, Thermo Fisher Scientific, Basel, Switzerland, #77600) were reconstituted and stored at −80 °C in single-use aliquots according to the manufacturer’s recommendations under sterile conditions. All biotherapeutics were bought from Runge Pharma GmbH & Co (Lörrach, Germany) in their respective formulation and stored according to the manufacturer’s recommendations. Peptides were synthesized by Cambridge Research Biochemicals and reconstituted in sterile ultra-pure water (Invitrogen, Basel, Switzerland, #10977015) and 50% Acetonitrile (≥99.95%, VWR, #83639.320). Biotherapeutics were used at a final concentration of 0.3 μM (peptides were used at a final concentration of 10 μg/mL) for both the DC stimulation stage and re-stimulation stage.

The fully human anti-interleukin (IL)-21 receptor (ATR-107) mAb was kindly gifted from Genentech. The marketed fully human mAb adalimumab (Humira^®, Abbvie, Chicago, IL, USA) was included as a clinical grade benchmark product purchased from a pharmacy. ATR-107 and adalimumab were stored at −80 °C and 4 °C, respectively. Stock solutions of the highly immunogenic antigen keyhole limpet hemocyanin (KLH-Imject Maleimide-Activated mcKLH; Thermo Fisher Scientific Inc, Waltham, MA, USA; Cat: #77600), which served as a positive control, were reconstituted in sterile water at 10 mg/mL and stored at 4 °C.