Ab241060

Manufactured by Abcam

Ab241060 is a laboratory product offered by Abcam. It is a primary antibody used in research applications. The core function of this product is to detect and bind to a specific target antigen.

Automatically generated - may contain errors

Lab products found in correlation

Ab51502, by Abcam (1 mentions) Ab230261, by Abcam (1 mentions) Ab76442, by Abcam (1 mentions) Ab19136, by Abcam (1 mentions) Ab237703, by Abcam (1 mentions) Ab51252, by Abcam (1 mentions) Mab1568, by Merck Group (1 mentions) Sc 23948, by Santa Cruz Biotechnology (1 mentions) Homer1, by Synaptic Systems (1 mentions) Mab377, by Merck Group (1 mentions) Rab10, by Cell Signaling Technology (1 mentions) Ab5541, by Merck Group (1 mentions) Vamp2, by Synaptic Systems (1 mentions) Sc 58774, by Santa Cruz Biotechnology (1 mentions) α synuclein, by BD (1 mentions) Cell tak, by Corning (1 mentions) K2 edta tubes, by Thermo Fisher Scientific (1 mentions) Opti mem reduced serum medium, by Thermo Fisher Scientific (1 mentions)

2 protocols using ab241060

Comprehensive Antibody Panel for Neuronal Characterization

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Rab10 (4262S, Cell Signaling Technology) for immunoblot, Rab10 (ab237703, Abcam) for immunofluorescence, pRab10 for immunoblot (ab230261, Abcam), pRab10 for immunofluorescence (ab241060, Abcam), Hsc70 (ab19136, Abcam), α-Tubulin (SC23948, Santa Cruz Biotechnology), parvalbumin, (NBP2-50036, NovusBio), NeuN (MAB377, Millipore), SATB2 (ab51502 Abcam), calretinin (MAB1568, Millipore), DARPP32 (MAB4230, R&D Systems), ChAT (NBP2-46620, NovusBio), DAT loop (6-8D6 Santa Cruz Biotechnology), tyrosine hydroxylase (TH) (ab76442, Abcam), CD68 (NBP2-33337SS, NovusBio), GFAP (AB5541, Millipore), Olig2 (MABN50 Millipore), KDEL receptor (sc-58774, Santa Cruz Biotechnology), TGN46 (MA3-063, ThermoFisher), LAMP1 (1D4B, DHSB), EEA1 (NBP2-36568, NovusBio), α-Synuclein (610786, BD Transduction Lab), Synuclein (ab51252, Abcam), VAMP2 (104 211, Synaptic Systems), Homer1 (160 006 Synaptic Systems), Rab8a (ab188574).

Singh V., Menard M.A., Serrano G.E., Beach T.G., Zhao H.T., Riley-DiPaolo A., Subrahmanian N., LaVoie M.J, & Volpicelli-Daley L.A. (2023). Cellular and subcellular localization of Rab10 and phospho-T73 Rab10 in the mouse and human brain. Acta Neuropathologica Communications, 11, 201.

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Rodent Plasma Isolation and Immunostaining

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Rodent blood was collected via cardiac stick in Cohorts 1–3. Approximately 1000 μl blood was extracted and transferred to K2 EDTA tubes (ThermoFisher Scientific, Waltham MA). Samples were spun at 2500 g for 20 min and the plasma was removed and stored. The buffy coat enriched fraction was collected and diluted in 1000 μl OptiMEM reduced serum medium (Thermo Fisher). Samples were then plated at 100 μl per well in 16-well chamber slides, which were pre-coated with Cell-Tak (Corning Inc., Corning NY). Chamber slides were centrifuged at 500 g for 5 min to promote cell adherence. Post centrifuge, media was removed, and cells were fixed with 4% PFA for 20 min at RT. Samples containing white blood cells (WBC) were stained using pThr73-Rab10 (ab241060, Abcam) overnight at 4 °C, and analyzed via confocal microscopy as described above.

De Miranda B.R., Castro S.L., Rocha E.M., Bodle C.R., Johnson K.E, & Greenamyre J.T. (2021). The industrial solvent trichloroethylene induces LRRK2 kinase activity and dopaminergic neurodegeneration in a rat model of Parkinson’s disease. Neurobiology of disease, 153, 105312.

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