All solvents and reagents were at least of reagent grade quality and were purchased either from Alfa Aesar, Sigma-Aldrich or Acros organics. 4,4,5,5,6,6,7,7,7-Nonafluoroheptanol was purchased from Fluorochem. Anhydrous DMF and CHCl3 were obtained from Acros organics. Gold coated silicon wafer (1000 Å layer thickness) and TBAPF6 (electrochemical grade) were purchased from Sigma-Aldrich. Polypropylene (biaxially oriented, 50 μm) was purchased from Goodfellow. The typical size of the surfaces reported in this paper was 1 cm2. Ultrapure water was obtained via a Millipore Milli-Q system (18.2 MΩ.cm).
Caution! Although we have not encountered any problem, it is noted that diazonium salts are potentially explosive and should be handled with appropriate precautions.
Gold coated silicon wafer
Manufactured by Merck Group
Gold-coated silicon wafers are a type of lab equipment used in various scientific and technological applications. These wafers are made of silicon, a widely used semiconductor material, and are coated with a thin layer of gold. The gold coating serves to enhance the conductivity and reflectivity properties of the silicon wafer, making it useful for a range of research and development purposes.
Automatically generated - may contain errors
Lab products found in correlation
Tbapf6, by Merck Group (1 mentions)
Anhydrous dmf, by Thermo Fisher Scientific (1 mentions)
Milli q system, by Merck Group (1 mentions)
Nascn, by Merck Group (1 mentions)
Natca, by Merck Group (1 mentions)
Acetamide, by Merck Group (1 mentions)
N methylacetamide, by Merck Group (1 mentions)
N methylurea, by Merck Group (1 mentions)
Milli q gradient water filtration system, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Acetic acid, by Merck Group (1 mentions)
Urea, by Merck Group (1 mentions)
Nitric acid, by Merck Group (1 mentions)
N methyl 2 pyrrolidone, by Merck Group (1 mentions)
Cary 660 spectrometer, by Agilent Technologies (1 mentions)
Cary 620 ftir microscope, by Agilent Technologies (1 mentions)
Dimethylformamide, by Merck Group (1 mentions)
5 protocols using gold coated silicon wafer
1
Diazonium Salt Functionalization of Surfaces
2
PNIPAM Nanocomposite Fabrication
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PNIPAM (number average molecular weight (Mn) of 475000 g/mol and polydispersity index (PDI = Mw/Mn) of 1.3) and mono thiol-terminated poly(N-isopropylacrylamide) (PNIPAM-SH, Mn = 8000 g/mol, PDI = 1.3) were purchased from Polymer Source Inc. (Dorval, Canada) and used as received. NaF (≥ 99%), NaTCA (≥ 97%), NaSCN (≥ 98.5%), and gold-coated silicon wafer (99.999% Au, layer thickness of 1000 Å) were supplied by Sigma-Aldrich. Goldcoated barium titanate glass microspheres (coating thickness: around 20 nm) were purchased from Cospheric LLC (Santa Barbara, USA). Degassed ultra-pure Milli-Q water with a resistivity of 18.2 MΩ•cm and organic content below 5 ppb was used. All PNIPAM solutions were prepared using a nutating mixer at 5 °C for 24 h.
3
Synthesis and Characterization of Lithium Cobalt Oxide
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Lithium cobalt oxide (LCO,
99.9%), N-methyl urea (NMU, 97%), acetamide (A, 99%),
urea (U, ACS reagent), N,N′-dimethylurea
(NN′DMU, 99%), N-methylacetamide (NMA, 99%), N-methyl-2-pyrrolidone (NMP), potassium nitrate (ACS reagent),
nitric acid, methanol (HPLC grade), and acetic acid (glacial) were
all purchased from Sigma-Aldrich and used without any purification
process unless otherwise mentioned. Gold-coated silicon wafers were
purchased from Sigma-Aldrich. NMU was recrystallized from methanol.
Ultrapure-grade water (resistivity 18.2 MΩ), purified using
the Milli-Q gradient water filtration system (Millipore), was used
for all purposes wherever necessary. Prior to usage, the Au thin films
were immersed in a piranha solution (3:1, H2O2 (30%)/H2SO4 (conc.) v/v) for 30 s, washed with 5 mL of water five times (fresh
water was replaced each time), dried flushed with N2 and
immediately used for analysis.
99.9%), N-methyl urea (NMU, 97%), acetamide (A, 99%),
urea (U, ACS reagent), N,N′-dimethylurea
(NN′DMU, 99%), N-methylacetamide (NMA, 99%), N-methyl-2-pyrrolidone (NMP), potassium nitrate (ACS reagent),
nitric acid, methanol (HPLC grade), and acetic acid (glacial) were
all purchased from Sigma-Aldrich and used without any purification
process unless otherwise mentioned. Gold-coated silicon wafers were
purchased from Sigma-Aldrich. NMU was recrystallized from methanol.
Ultrapure-grade water (resistivity 18.2 MΩ), purified using
the Milli-Q gradient water filtration system (Millipore), was used
for all purposes wherever necessary. Prior to usage, the Au thin films
were immersed in a piranha solution (3:1, H2O2 (30%)/H2SO4 (conc.) v/v) for 30 s, washed with 5 mL of water five times (fresh
water was replaced each time), dried flushed with N2 and
immediately used for analysis.
4
FT-IR Spectroscopic Imaging of ECM Adsorption
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Poly(styrene-co-4-styrene sulfonic acid) (33% PSS [mol% of SO3H: 33], Polymer Source, USA) was dissolved in dimethylformamide (Sigma Aldrich, Korea). For FTIR imaging, 33% PSS solutions were spin-coated onto gold-coated silicon wafers (Sigma) at 2000 rpm for 1 min. The PSS-coated surfaces were annealed at 150 °C overnight. After annealing, the surfaces were incubated with 66 µg/ml FN (Invitrogen, USA), LAM (Invitrogen, USA), type-Ι COL (Sigma Aldrich, Korea), or ELAS (Sigma Aldrich, Korea) in phosphate-buffered saline (pH 7.4) for 72 h at 37 °C. After incubation, the samples were washed with deionized water and dried in a desiccator. FT-IR images of the dried samples were acquired from the collected spectra, which were obtained using a Cary 660 spectrometer and a Cary 620 FT-IR microscope (Agilent Technologies, Korea). The resolution and total number of scans of the spectra were 4 cm−1 and 32, respectively, in the 800–4,000 cm−1 wavenumber regions with Agilent’s 16 × 16 focal plane array. Note that the background was corrected for each measurement.
5
Diazonium Salt Functionalization of CVD Graphene
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Commercially available CVD graphene, which was grown on copper substrates and transferred onto gold-coated silicon wafers (from Sigma-Aldrich), was purchased from 2-DTech. The diazonium salt was synthesised from aniline and sodium nitrite following the procedure used by Lackner and Fu ¨rstner. 30 The benzene diazonium salt (0.80 g, 7.61 mmol) was added to a stirred solution of sodium dodecyl sulphate (1 wt%, 10 mL, Alfa Aesar), and the solution was heated (35 1C) to allow the salt to dissolve. The graphene sample was submersed in the solution and left to react for 24 hours. We also prepared control samples by immersing CVD graphene in pure aniline solution (i.e. without the addition of nitrite such that no diazonium salt was formed) for 24 hours. After the reaction, the substrates were washed (ultra-filtrated water) and dried in nitrogen gas. 8
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