The obtained tissue sections (10 μm sheet thickness) were also co-incubated with primary CD14 (1: 200) antibody (Rabbit Polyclonal, proteintech, China) overnight at a 4 °C refrigerator. After washing with detergent (P0106, Beyotime, China) three times for 5 min, the samples were co-incubated again with Cy3 secondary antibody (1: 50, SA00009-1, proteintech, China) and dark atmosphere overnight at a 4 °C refrigerator. The samples were observed by a fluorescence microscope.
P0106
Manufactured by Beyotime
Sourced in China
The P0106 is a laboratory equipment designed for general use in research and scientific applications. It serves as a basic tool for various experimental procedures. The core function of the P0106 is to provide a stable and controlled environment for conducting experiments.
Automatically generated - may contain errors
Lab products found in correlation
P0102, by Beyotime (2 mentions)
Sa00009 1, by Proteintech (1 mentions)
Tunel staining kit, by Beyotime (1 mentions)
Bx53 optical microscope, by Olympus (1 mentions)
C1091, by Beyotime (1 mentions)
Proteinase k, by Beyotime (1 mentions)
Eclipse ti2 microscope, by Nikon (1 mentions)
Af1738, by Beyotime (1 mentions)
Signalstain boost immunohistochemistry detection reagent, by Cell Signaling Technology (1 mentions)
G1101, by Wuhan Servicebio Technology (1 mentions)
Protease k without dnase, by Beyotime (1 mentions)
One step tunel apoptosis assay kit, by Beyotime (1 mentions)
P0099, by Beyotime (1 mentions)
Mvx10, by Olympus (1 mentions)
Edu cell proliferation kit, by Beyotime (1 mentions)
Ab6721, by Abcam (1 mentions)
8 protocols using p0106
1
Immunofluorescence Staining of CD14
2
Detecting Apoptosis in Heart Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The TUNEL staining kit (C1091, Beyotime, Shanghai, China) was used in this study. After being incubated with 20 μg/ml of Proteinase K (M049338, MREDA, Beijing, China), the slices of heart tissue were sequentially immersed in washing buffer (P0106, Beyotime) at 20°C for 20 min (min) and blocked using blocking solution (P0100B, Beyotime) at room temperature for 20 min. Subsequently, the tissue slices were first incubated with 0.05 ml of marker buffer (provided in the kit) at 37°C for 1 h in the dark and then with stop buffer at room temperature for 10 min. After that, the tissue slices were incubated with Streptavidin-HRP solution (provided in the kit) for 30 min and stained with 0.3 ml of DAB buffer (provided in the kit) at room temperature for 20 min. After washing with PBS (M059191, MRREDA) for three times, the images of the tissue slices were observed under an optical microscope (DM4M, Leica, Solms, Germany) at a magnification of ×200.
3
Apoptosis Analysis in Tumor Tissues
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell apoptosis in mice tumor tissues was examined by TUNEL staining using a reaction kit (c1091, Beyotime) which contained marker buffer, streptavidin-HRP solution, and DAB buffer. In brief, after being fixed on a glass slide, the mice tumor tissue slices were incubated with Proteinase K (ST532, Beyotime) for 20 min, followed by 20 min incubation with wash buffer (P0106, Beyotime). Then the tissue slices were blocked with block solution (P0100B, Beyotime) for 20 min. After 60 min of incubation with the marker buffer in the dark, the tissue slices were covered with stop buffer for 10 min. Then the streptavidin-HRP solution and the DAB buffer were separately used to incubate the tissue slices for 30 min. After washing with PBS, the image of the tissue slices was recorded with a BX53 optical microscope (Olympus, Japan).
4
Immunohistochemical Analysis of Ki-67 Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The tumors were fixed in 4% paraformaldehyde solution (30525-89-4, Aladdin, China) for 48 h at room temperature, embedded in paraffin and then sectioned into 4 μm thick. Slides were deparaffinized in a series of xylene (1330-20-7, Aladdin, China) and graded alcohols. After that, the sections were heated in citrate buffer (10mM, pH 6.0) in sub-boiling temperature for 10 min for antigen retrieval. Next, the sections were incubated with primary antibody (Ki-67, AF1738, 1:400, Beyotime, China), which was diluted in immunol staining blocking buffer (P0102, Beyotime, China) for 2h at 4°C. After that, the sections were washed by wash buffer (P0106, Beyotime, China) for 15min twice at room temperature and incubated with SignalStain® Boost IHC Detection Reagents (HRP, 8114S, CST, China) at room temperature for 30min. Then, the slices were observed under an ECLIPSE Ti2 microscope (Ti2-U, Nikon, Japan).
5
Immunohistochemical Analysis of Ki-67
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The sections were fixed at room temperature with 4% paraformaldehyde (30,525–89-4, Aladdin, Shanghai, China) for 48 h, embedded in paraffin, and cut into 4-μm thickness sections. Sections were then dewaxed in gradient concentrations of xylene (1330–20-7, Aladdin) and ethanol. Afterwards, the sections were heated in a citrate buffer (10 mM, pH = 6.0) below boiling temperature for a period of 10 min for antigen retrieval. Next, the sections were probed with the primary antibody against Ki-67 (AF1738, 1:400, Beyotime) and diluted in immunostaining blocking buffer (P0102, Beyotime) for 4°C for 2 h. Subsequently, the sections were washed two times at ambient temperature with washing buffer (P0106, Beyotime) for 15 min and subjected to a 30-min incubation with SignalStain® Boost immunohistochemistry detection reagent (horseradish peroxidase-labelled, 8114S, Cell Signaling Technologies, Beverly, MA, USA) at room temperature. Finally, sections were observed under an inverted microscope (Olympus IX-71, Tokyo, Japan).
6
TUNEL Assay for Apoptosis Detection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Retina and ARPE-19 cell apoptosis levels were detected using the One-Step TUNEL Apoptosis Assay Kit, according to the procedures from the manufacturer (C1089, Beyotime, China). According to the manufacturer’s instructions, paraffin-embedded eyeballs were dewaxed in xylene (5–10 min). Next, xylene was replaced, and dewaxing was performed for 5–10 min in the following sequence: anhydrous ethanol (5 min), 90% ethanol (2 min), 70% ethanol (2 min), and distilled water (2 min). The cells were fixed with 4% paraformaldehyde (G1101, Servicebio, China) for 30 min. Then, 20 μg/mL of protease K without DNase (ST532, Beyotime, China), immunostaining washing solution (P0106, Beyotime, China), and 50 μL of TUNEL test solution were added for 60 min in the dark.
7
Quantifying NPC Proliferation using EdU Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The proliferation of NPCs was measured by the EdU Cell Proliferation Kit (C0071S, Beyotime, Shanghai, China). Prior to this assay, the Click Additive Solution, EdU reagent, and Hoechst 33342 reagent were prepared with the help of EdU Cell Proliferation Kit. NPCs were maintained in 6-well plates (5 × 105 cells/well) and transfected as instructed. Cells were then incubated with EdU reagent at 37°C for 2 h, after which these cells were fixed with 4% paraformaldehyde (P0099, Beyotime, Shanghai, China) and washed with wash buffer as appropriate. Afterwards, cells were incubated with immunol staining wash buffer (P0106, Beyotime, Shanghai, China) at room temperature for 15 min, and then treated with Click Additive Solution for 30 min in the dark, followed by the staining with Hoechst 33342 reagent at room temperature for 10 min in the dark. Finally, cells were washed with wash buffer and observed (magnification 200×) under a fluorescence microscope (MVX10, OLYMPUS, Tokyo, Japan).
8
CD34 Immunohistochemistry Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The obtained tissue sections (10 μm sheet thickness) were co-incubated with primary CD34 (1: 200) antibody (Rabbit Polyclonal, proteintech, China) overnight at a 4 °C refrigerator. After washing with detergent (P0106, Beyotime, China) three times for 5 min, the samples were co-incubated again with an HRP-conjugated secondary antibody (1: 500, ab6721, Abcam, Cambridge) for 60 min at room temperature. The expression was observed with 3, 3-diaminobenzidine (DAB) staining.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!