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Hep3b

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Hep3B is a laboratory cell line derived from a human hepatocellular carcinoma. It is a widely used in vitro model for the study of liver cancer and related research.

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Lab products found in correlation

Sk hep1, by American Type Culture Collection (1 mentions) Sirna oligonucleotide, by GenePharma (1 mentions) Hepg2, by American Type Culture Collection (1 mentions) Hep3b, by American Type Culture Collection (1 mentions) Lipofectamine 3000, by GenePharma (1 mentions) Smmc7721, by GenePharma (1 mentions) Hepg2, by GenePharma (1 mentions)

2 protocols using hep3b

1

RNA Interference Modulates HCC Cell Lines

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The HCC cell lines HepG2, SK‐Hep1, and Hep3B used for this study were purchased from ATCC (Rockville, MD, USA) and stored at liquid nitrogen. All these cells were cultured in DMEM supplemented with 10% heat‐inactivated fetal bovine serum in incubators with a humidified atmosphere of 5% CO2 and 95% air at 37 °C. For RNAi in Hep3B cells, siRNA oligonucleotides targeting DNMT1, DNMT3A, and DNMT3B were ordered from GenePharma, and transfected into cells with Lipofectamine 3000.
Meng J., Li S., Niu Z., Bao Z, & Niu L. (2023). The efficacy of sorafenib against hepatocellular carcinoma is enhanced by 5‐aza‐mediated inhibition of ID1 promoter methylation. FEBS Open Bio, 14(1), 127-137.
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2

Human HCC Cell Culture Protocol

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Hep1, HepG2, Hep3B, Huh7, and SMMC7721 human HCC cells and L02 normal liver cells purchased from GenePharma Technology Co., Ltd were studied in our research. Cells were cultured in DMEM supplemented with 10% FBS and 1% penicillin/streptomycin in a humidified 5% CO2 incubator at 37°C.
Cao P., Ma B., Sun D., Zhang W., Qiu J., Qin L, & Xue X. (2021). hsa_circ_0003410 promotes hepatocellular carcinoma progression by increasing the ratio of M2/M1 macrophages through the miR‐139‐3p/CCL5 axis. Cancer Science, 113(2), 634-647.
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