Abi 491

Manufactured by Thermo Fisher Scientific

The ABI 491 is a laboratory instrument used for automated DNA sequencing. It is designed to perform high-throughput DNA sequencing analysis. The core function of the ABI 491 is to determine the precise order of nucleotides within DNA samples.

Automatically generated - may contain errors

Lab products found in correlation

Axima cfr mass spectrometer, by Shimadzu (3 mentions)

4 protocols using abi 491

Peptide Identification and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

N-terminal sequence of the purified peptide was determined by Edman degradation on an Applied Biosystems pulsed liquid-phase sequencer (model ABI 491). Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used to identify the purified peptide. AXIMA CFR mass spectrometer (Kratos Analytical) was analyzed in linear and positive ion mode using an acceleration voltage of 20 kV and an accumulating time of single scanning of 50 s.

Mu L., Zhou L., Yang J., Zhuang L., Tang J., Liu T., Wu J, & Yang H. (2017). The first identified cathelicidin from tree frogs possesses anti-inflammatory and partial LPS neutralization activities. Amino Acids, 49(9), 1571-1585.

+ Open protocol

+ Expand

Peptide Sequence Identification by MALDI-TOF

Check if the same lab product or an alternative is used in the 5 most similar protocols

The N-terminal sequence of the purified peptide was determined by Edman degradation on an Applied Biosystems pulsed liquid-phase sequencer (model ABI 491). Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used to identify the purified peptide. The AXIMA CFR mass spectrometer (Kratos Analytical) was analyzed in the linear and the positive ion mode using an acceleration voltage of 20 kV and an accumulating time of single scanning of 50 s.

Wu J., Yang J., Wang X., Wei L., Mi K., Shen Y., Liu T., Yang H, & Mu L. (2018). A frog cathelicidin peptide effectively promotes cutaneous wound healing in mice. Biochemical Journal, 475(17), 2785-2799.

+ Open protocol

+ Expand

Peptide Sequencing and Purity Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

N-terminal sequence of the purified peptide was determined by Edman degradation on an Applied Biosystems pulsed liquid-phase sequencer (model ABI 491). Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used to identify the purity of the isolated peptide. AXIMA CFR mass spectrometer (Kratos Analytical) was analyzed in linear and positive ion mode using an acceleration voltage of 20 kV and an accumulating time of single scanning of 50 s.

Feng G., Wei L., Che H., Shen Y., Yang J., Mi K., Liu J., Wu J., Yang H, & Mu L. (2022). A Frog Peptide Ameliorates Skin Photoaging Through Scavenging Reactive Oxygen Species. Frontiers in Pharmacology, 12, 761011.

+ Open protocol

+ Expand

Purification and Characterization of SibaCec

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

According to the methods in our previous report [11 (link)], the eluted peak of A1 (Fig. 1a) containing antimicrobial activity was pooled, lyophilized, and further purified by RP-HPLC on a Wondasil C₁₈ column (25 × 0.46 cm). The elution was performed using a linear gradient of 0–60 % acetonitrile containing 0.1 % (v/v) trifluoroacetic acid in 0.1 % (v/v) trifluoroacetic acid/water over 70 min. N-terminal sequence of the purified peptide was done by Edman degradation on an Applied Biosystems pulsed liquid-phase sequencer (model ABI 491).Fig. 1

Purification of SibaCec from the salivary gland of S. bannaense and MALDI–TOF MS. a The filtrate of the salivary gland homogenate of S. bannaense was divided by an Inertsil C₄ RP-HPLC column. b The eluted peak of A1 containing antimicrobial activity was further purified by C₁₈ RP-HPLC column. The purified SibaCec is indicated by an arrow. c MALDI-TOF mass spectrometry analysis of the purified SibaCec

Wu J., Mu L., Zhuang L., Han Y., Liu T., Li J., Yang Y., Yang H, & Wei L. (2015). A cecropin-like antimicrobial peptide with anti-inflammatory activity from the black fly salivary glands. Parasites & Vectors, 8, 561.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!