Supernatants from the cell cultures were harvested at designated times post infection. The IL-13 content in the supernatants was analyzed by using porcine IL-13 ELISA kit (RayBiotech) and human IL-13 ELISA kit (Abcam), following the manufacturers’ instructions.
Human il 13 elisa kit
Manufactured by Abcam
Sourced in United States
The Human IL-13 ELISA kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of human interleukin-13 (IL-13) in cell culture supernatants, serum, and plasma samples. The kit utilizes a specific antibody coated on a microplate to capture IL-13 from the samples, and a detection antibody conjugated with an enzyme for colorimetric quantification.
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Lab products found in correlation
2 protocols using human il 13 elisa kit
1
Measurement of IL-13 in Cell Cultures
2
Serum Biomarkers in Allergy Study
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Venous blood samples were collected from relative participants. Blood samples were centrifuged at 1500 rpm for 15 min to collect the serum. All samples were stored at -80°C.
Serum vitamin E level was evaluated through LC-20AD high-performance liquid chromatography (Shimadzu, Japan). Serum vitamin E level was adjusted by total cholesterol and triglycerides.
The serum levels of interleukin (IL)-13 and IL-4 were measured by Human IL-13 ELISA Kit (ab178014, Abcam, Cambridge, MA, USA) and Human IL-4 ELISA Kit (ab215089, Abcam) based on the manufacturer's instructions.
The serum level of total IgE was measured by fluorescence enzyme immunoassay using Immuno-CAP 100 (Phadia, Uppsala, Sweden).
Serum vitamin E level was evaluated through LC-20AD high-performance liquid chromatography (Shimadzu, Japan). Serum vitamin E level was adjusted by total cholesterol and triglycerides.
The serum levels of interleukin (IL)-13 and IL-4 were measured by Human IL-13 ELISA Kit (ab178014, Abcam, Cambridge, MA, USA) and Human IL-4 ELISA Kit (ab215089, Abcam) based on the manufacturer's instructions.
The serum level of total IgE was measured by fluorescence enzyme immunoassay using Immuno-CAP 100 (Phadia, Uppsala, Sweden).
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