Microsoft excel 2017

Western blotting, RT-PCR’s and Immunofluorescence experiments were replicated at least three times with individually passaged cells, and data sets were expressed as means ± standard deviations (SD). Statistically significant differences were compared using the unpaired Student’s t-test. P values: ***P < 0.001; **P < 0.01; *P < 0.05 were considered statistically as significant. All analyses were performed with the software Microsoft Excel 2017 and Graphpad Prism Version 5 (http://www.graphpad.com/scientific-software/prism/).

Western blotting, RT-PCR’s and Immunofluorescence experiments were replicated at least three times with individually passaged cells, and data sets were expressed as means ± standard deviations (SD). Statistically significant differences were compared using the unpaired Student’s t-test. P values: *** P < 0.001; ** P < 0.01; * P < 0.05 were considered statistically as significant. All analyses were performed with the software Microsoft Excel 2017 and Graphpad Prism Version 5 (http://www.graphpad.com/scientific-software/prism/). All statistical tests on metabolite profiles have been conducted in R (www.r-project.org) using the respective functions. For principal component analysis we used the pcaMethods package, applying the nipals algorithm on pareto normalized data. For analysis of variance (ANOVA) we considered genotype and treatment as factors and allowing for interactions. The resulting P-values were ranked and corrected according to the original FDR method of Benjamini-Hochberg (Desired FDR (Q) = 5%) with GraphPad Prism 8 (www.graphpad.com).