The following reagents were purchased commercially: Poly-L -lysine, dbcAMP, forskolin, and the pharmacological Trk receptor inhibitor K252a (Sigma-Aldrich); recombinant human BDNF (PeproTech); and the pharmacological PKA inhibitor H-89 (Cell Signaling Technology). We used the following antibodies: a rabbit polyclonal antibody for R-Ras was obtained as described previously [8] (link). The other antibodies were purchased commercially: mouse monoclonal antibodies againstα-tubulin (B-5-1-2) and Flag (M2; Sigma-Aldrich); rabbit polyclonal antibodies against ERK and phospho-RasGRF1 (Ser916/898), and a rabbit monoclonal anti-T202/Y204 phospho-ERK antibody (Cell Signaling Technology); a rabbit polyclonal antibody against RasGRF1 (C-20; Santa Cruz Biotechnology); Alexa Fluor-conjugated secondary antibodies (Invitrogen); and horseradish peroxidase (HRP)-conjugated secondary antibodies (Dako).
Pka inhibitor h 89
Manufactured by Cell Signaling Technology
Sourced in United States
PKA inhibitor H-89 is a cell-permeable, selective, and potent inhibitor of protein kinase A (PKA). It functions by competitively inhibiting the ATP-binding site of the PKA catalytic subunit.
Automatically generated - may contain errors
Lab products found in correlation
B 5 1 2, by Merck Group (1 mentions)
Horseradish peroxidase hrp conjugated secondary antibody, by Agilent Technologies (1 mentions)
Recombinant human bdnf, by Thermo Fisher Scientific (1 mentions)
Alexa fluor conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions)
Poly l lysine (pll), by Merck Group (1 mentions)
Aicar, by Beyotime (1 mentions)
Exendin 9 39, by Merck Group (1 mentions)
Liraglutide, by Novo Nordisk (1 mentions)
2 protocols using pka inhibitor h 89
1
Neuronal Signaling Pathway Modulation
2
Molecular Mechanisms of Liraglutide's Cardioprotective Effects
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Liraglutide was provided by Novo Nordisk (Bagsvaerd, Denmark). PA, AMPK inhibitor compound C and GLP-1R antagonist exendin (9–39) were purchased from Sigma (St. Louis, MO, USA). AMPK activator AICAR was from Beyotime Biotechnology (Shanghai, China). PKA inhibitor H89 was provided by Cell Signalling Technology (Beverly, MA, USA). Mouse anti-eNOS and anti-phospho-Ser-1177-eNOS (p-eNOS) antibodies were from BD Biosciences (San Joe, CA, USA). Mouse anti-GLP-1R monoclonal antibody was from DSHB (Iowa, IA, USA). Rabbit anti-PKA Cα, anti-phospho-Thr197-PKA C (p-PKA), anti-AMPKα and anti-phospho-Thr-172-AMPKα (p-AMPK) antibodies were obtained from Cell Signalling Technology. Mouse anti-GAPDH antibody from Zhongshan Biotechnology (Beijing, China).
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