Mouse IL-6 (431401), IL-10 (431411) and TNFα (430901) ELISA kits and Anti-F4/80 mouse BV421 antibody (123131) were purchased from BioLegend (San Diego, CA). Mouse CXCL1/KC ELISA kit (DY453-05) was purchased from R&D Systems (Minneapolis, MN). Collagenase (234155) was purchased from EMD Millipore (Billerica, MA). Anti-CD206 mouse Alexa Fluor 647 antibody (MCA2235A647T) was purchased from AbD Serotec (Raleigh, NC). Anti-Ly-6G (Gr-1) mouse PE antibody (553128) was purchased from BD Pharmingen (San Jose, CA). Blood agar base (70133), Hemin (H9039), and b-Nicotinamide Adenine Dinucleotide hydrate (NAD) (N3014) were purchased from Sigma (St. Louis, MO). Brain Heart Infusion (237500) and anti-CD80 mouse PerCP-Cy5.5 antibody (560526) were purchased from BD Bioscience (San Jose, CA). PGE2 and AT-RvD1 (7S,8R,17R-trihydroxy-4Z,9E,11E,13Z,15E, 19Z-docosahexaenoic acid) were purchased from Cayman Chemical (Ann Arbor, MI). Secondary Western blot antibodies (115-035-146, 111-035-144) were purchased from Jackson ImmunoResearch Laboratories (West Grove, PA). PBS (14200-075) was purchased from Gibco (Waltham, MA).
Pbs 14200 075
Manufactured by Thermo Fisher Scientific
PBS (14200-075) is a sterile, isotonic phosphate-buffered saline solution. It is commonly used as a general-purpose buffer in various laboratory applications.
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Lab products found in correlation
2 protocols using pbs 14200 075
1
Murine Immune Response Assay
2
Characterization of hADSC Surface Markers
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Specific cell markers expressed in hADSCs were detected for purity. To identify surface proteins, hADSCs were collected and adjusted to 2 × 10 5 cells/ml. After washing with phosphate-buffered saline (PBS, 14200-075; Gibco) once, cells were resuspended in 50 ml PBS and stained by 1 ml of surface protein-specific antibodies conjugated with fluorescent dyes in a dark environment for 30 min. Prepared cell samples were analyzed by LRS II flow cytometry (BD Pharmingen™, Franklin Lakes, NJ, USA) for expression levels of cell surface proteins. Surface proteins in hADSCs possessed the following surface proteins: cluster of differentiation 29 (CD29), CD44, CD73, CD90, CD105, and CD166 (BD Pharmingen™, San Jose, CA, USA). In the meantime, hADSCs tested negative for CD14, CD45, and CD31 (AbD Serotec, Raleigh, NC, USA).
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