Trifluoroacetic acid, tris
(2-carboxyethyl) phosphine (TCEP), iodoacetamide (IAA), α-methylmannoside,
α-methylglucoside, TEAB, Bradford reagents, water [high-performance
liquid chromatography (HPLC) grade], formic acid (FA) (HPLC grade),
and acetonitrile (HPLC grade) were purchased from Sigma-Aldrich (St.
Louis, MO). The Pierce centrifuge column (2 mL), centrifuge filters
(0.45 μm), C18 spin column, and low binding tips were purchased
from Thermo Fisher Scientific (Fair Lawn, NJ). Low protein-binding
tubes were purchased from Eppendorf (Hauppauge, NY). Agarose-bound Lens culinaris agglutinin (LCA) was purchased from
Vector Laboratories (Burlingame, CA). Amicon ULtra 3 K (0.5 and 4.0
mL) and 10 k (4.0 mL) centrifugal filters were purchased from Millipore
(Billerica, MA). The Bradford assay kit was purchased from BioRad
(Hercules, CA). iTRAQ 8-plex reagent kits were purchased from AB Sciex
(Redwood, CA). Sequencing-grade modified trypsin enzyme was obtained
from Promega (Madison, WI). Endoglycosidase F3 (Endo F3) was purchased
from BA-Bio (San Mateo, CA). Human 14 multiple affinity removal system
spin cartridges for the depletion of high-abundant proteins from human
proteomic samples were from Agilent (Santa Clara, CA).
(2-carboxyethyl) phosphine (TCEP), iodoacetamide (IAA), α-methylmannoside,
α-methylglucoside, TEAB, Bradford reagents, water [high-performance
liquid chromatography (HPLC) grade], formic acid (FA) (HPLC grade),
and acetonitrile (HPLC grade) were purchased from Sigma-Aldrich (St.
Louis, MO). The Pierce centrifuge column (2 mL), centrifuge filters
(0.45 μm), C18 spin column, and low binding tips were purchased
from Thermo Fisher Scientific (Fair Lawn, NJ). Low protein-binding
tubes were purchased from Eppendorf (Hauppauge, NY). Agarose-bound Lens culinaris agglutinin (LCA) was purchased from
Vector Laboratories (Burlingame, CA). Amicon ULtra 3 K (0.5 and 4.0
mL) and 10 k (4.0 mL) centrifugal filters were purchased from Millipore
(Billerica, MA). The Bradford assay kit was purchased from BioRad
(Hercules, CA). iTRAQ 8-plex reagent kits were purchased from AB Sciex
(Redwood, CA). Sequencing-grade modified trypsin enzyme was obtained
from Promega (Madison, WI). Endoglycosidase F3 (Endo F3) was purchased
from BA-Bio (San Mateo, CA). Human 14 multiple affinity removal system
spin cartridges for the depletion of high-abundant proteins from human
proteomic samples were from Agilent (Santa Clara, CA).