Total organic nitrogen (TON), total organic carbon (TOC), total inorganic nitrogen (TIN) and total inorganic carbon (TIC) content were determined within a plaque and in healthy biocrust within 5 cm from the edge of the plaque, from cores with depth and diameter of 0.5 cm. Cores were ground to a fine powder in a SPEX Certiprep 8000D mill and milled for 5 minutes. For TOC/TIC we used the acid fumigation on a Perkin Elmer series II CHNS/O analyzer. For TON and TIN content, milled cores as above were used for the potassium chloride (KCl) extraction of nitrate and ammonium or for total nitrogen (TN) on a combustion analyzer.
Series 2 chns o analyzer
Manufactured by PerkinElmer
Sourced in United States
The Series II CHNS/O analyzer is a laboratory equipment designed to determine the elemental composition of organic and inorganic materials. It can accurately measure the carbon, hydrogen, nitrogen, sulfur, and oxygen content of a sample.
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4 protocols using series 2 chns o analyzer
1
Soil Nutrient Profiling in Biocrusts
2
Comprehensive Characterization of Adsorbent Materials
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The functional groups on the surface of the adsorbent were determined using FTIR (FTIR, Perkin Elmer, spectrum RXI 83303, MA, USA). Elemental analysis was achieved using Perkin Elmer series II CHNS/O analyzer (Perkin Elmer, 2400, MA, USA). Surface morphology was studied using SEM (SEM, JEOL JSM-6360LV, Tokyo, Japan) coupled with EDS (EDS, Thermo Noran, 6714A-ISUS-SN, WI, USA). Further structural information was obtained using X-ray diffraction (XRD-7000 X-Ray diffractometer, Shimadzu, Tokyo, Japan) with filtered Cu Kα radiation operated at 40 kV and 40 mA. The XRD pattern was recorded from 10 to 80 °C of 2θ per second with a scanning speed of 2.0000° of 2θ per minute. Zeta potential was determined using a zeta potential analyzer (DT1200, Dispersion technology, NY, USA) and thermal stability and fraction of volatile components was monitored using DTA-TG apparatus (C30574600245, Shimadzu, Tokyo, Japan). The surface area was determined by nitrogen adsorption at 373 K using BET method in a Quantachrome Autosorb 1 instrument (10902042401, Florida, USA).
3
Synthesis and Characterization of Ligands
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All reagents were commercial grade and were used
without further purification. The ligands2 and 3 were prepared as previously reported by our group.27 (link) NMR spectra were recorded with a Bruker Avance
III 500 MHz spectrometer. The 1H and 13C NMR
chemical shifts are reported in parts per million (ppm) relative to
the central line of the acetone-d6 signal
(2.05 ppm for 1H and 29.8 ppm for 13C). The 19F NMR chemical shifts are reported relative to the signal
of trifluoromethylbenzene (−63.22 ppm). IR spectra were obtained
with a Bruker ALPHA FT-IR spectrophotometer. High-resolution mass
spectra were recorded with a Thermo Fisher Q-Exactive instrument,
while mass spectra were recorded with a Micromass Waters Q-TOF Premier
instrument. Elemental analyses (C, H, N) were performed with a PerkinElmer
2400 Series II CHNS/O Analyzer.
without further purification. The ligands
III 500 MHz spectrometer. The 1H and 13C NMR
chemical shifts are reported in parts per million (ppm) relative to
the central line of the acetone-d6 signal
(2.05 ppm for 1H and 29.8 ppm for 13C). The 19F NMR chemical shifts are reported relative to the signal
of trifluoromethylbenzene (−63.22 ppm). IR spectra were obtained
with a Bruker ALPHA FT-IR spectrophotometer. High-resolution mass
spectra were recorded with a Thermo Fisher Q-Exactive instrument,
while mass spectra were recorded with a Micromass Waters Q-TOF Premier
instrument. Elemental analyses (C, H, N) were performed with a PerkinElmer
2400 Series II CHNS/O Analyzer.
4
Physicochemical Characterization of Compounds
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Melting point was determined on a Kofler micro hot stage and is uncorrected. 1 (link) H NMR spectrum was recorded with a Bruker Avance DPX 300 spectrometer at 29 °C and 300 MHz, using TMS as an internal standard. 13 (link) C NMR spectrum was recorded on the same instrument at 75.5 MHz and is referenced against the central line of the solvent signal (DMSO-d 6 septet at δ = 39.5 ppm). IR spectrum was obtained with Bio-Rad FTS 3000MX (KBr pellet). MS spectrum was recorded with a VG-Analytical Au-toSpec Q instrument. Elemental analysis (C, H, N) was performed with Perkin Elmer 2400 Series II CHNS/O Analyzer.
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