The quality of all chemicals was of analytical grade, unless stated otherwise. Disodium hydrogen phosphate dihydrate, ethanol, formic acid (FA; 98–100%), hydrochloric acid (32%, w/w), pentane, 1-propanol, potassium dihydrogen phosphate, sodium chloride, tris(hydroxymethyl)-aminomethane (TRIS), and urea were purchased from Merck (Darmstadt, Germany). IDAM was from Applichem (Darmstadt, Germany). α-Chymotrypsin (from bovine pancreas, TLCK-treated, ≥40 U/mg protein), pepsin (from porcine gastric mucosa, 3200–4500 U/mg protein), TCEP, trifluoroacetic acid (TFA; 99%), and trypsin (from bovine pancreas, TPCK-treated, ≥10 000 BAEE U/mg protein) were obtained from Sigma-Aldrich (Steinheim, Germany), and deuterated methanol-d4 containing tetramethylsilane (TMS) was from Euriso-Top (Gif sur Yvette Cedex, France). The peptide LQLQPFPQPQLPYPQPQLPYPQPQPLPYPQPQPF (33-mer) and the isotopically labelled peptide LQLQP*FPQPQLPYPQPQLPYPQPQLPYPQ*PQ*P*F (*33-mer) with *P: L-[13C5][15N]-proline and *F: L-[13C9][15N]-phenylalanine, were purchased from Genscript (Hongkong, PR China) with a purity of >90%. Water for HPLC was purified using an Arium 611VF water purification system (Sartorius, Goettingen, Germany).
Arium 611vf water purification system
Manufactured by Sartorius
Sourced in Germany
The Arium 611VF is a water purification system designed to produce ultrapure water for laboratory applications. It utilizes a combination of filtration and purification technologies to deliver high-quality water that meets specific purity requirements. The core function of the Arium 611VF is to provide a reliable and consistent source of purified water for various laboratory processes and experiments.
Automatically generated - may contain errors
Lab products found in correlation
Trypsin, by Merck Group (3 mentions)
Pepsin, by Merck Group (2 mentions)
α chymotrypsin, by Merck Group (2 mentions)
Potassium dihydrogen phosphate, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Ethanol, by Merck Group (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
Trifluoroacetic acid, by Merck Group (1 mentions)
Urea, by Merck Group (1 mentions)
Pentane, by Merck Group (1 mentions)
1 propanol, by Merck Group (1 mentions)
Disodium hydrogen phosphate dihydrate, by Merck Group (1 mentions)
Formic acid, by Merck Group (1 mentions)
Thermolysin, by Merck Group (1 mentions)
Deuterium oxide d2o, by Merck Group (1 mentions)
Toluene, by Avantor (1 mentions)
D glucose, by Merck Group (1 mentions)
Anhydrous sodium acetate, by Merck Group (1 mentions)
Sucrose, by Merck Group (1 mentions)
D fructose, by Merck Group (1 mentions)
4 protocols using arium 611vf water purification system
1
Peptide Characterization by Mass Spectrometry
2
Enzymatic Hydrolysis of Wheat Proteins
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Gluten and HWPs were from Hermann Kröner GmbH (Ibbenbüren, Germany), Tate & Lyle PLC (London, UK), and Manildra Group (Gladesville, Australia). G in the present study corresponds to G1, G-ω5 to G4, sHWP to HWP7, and eHWP to HWP3 (7 (link)). All reagents and chemicals were from Sigma Aldrich (Darmstadt, Germany), Merck (Darmstadt, Germany), Carl Roth (Karlsruhe, Germany), Honeywell (Offenbach, Germany), J. T. Baker (Arnhem, The Netherlands), and Fresenius Kabi Deutschland GmbH (Bad Homburg, Germany). Water was purified with an Arium 611VF water purification system (Sartorius, Goettingen, Germany). Pepsin (from porcine mucosa, 10 FIP U/mg), trypsin (from bovine pancreas, TPCK treated, 10.000 BAEE U/mg protein), α-chymotrypsin (from bovine pancreas, TLCK-treated, ≥40 U/mg protein), and thermolysin (from Geobacillus stearothermophilus, 30–175 U/mg protein) were purchased from Sigma Aldrich (Darmstadt, Germany) and Merck (Darmstadt, Germany).
3
Preparation of Fluorinated Glass Slides
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Glass slides of 26 mm
× 76 mm in size
were provided by Menzel-Gläser, Germany. Tetraethoxysilane
(TEOS, reagent grade, 98%), Trichloro (1H, 1H, 2H, and 2H-perfluorooctyl)
silane (PFOTS, 97%), hexadecane (reagentPlus, 99%), and deuterium
oxide (D2O, 99.9 atom % D) were purchased from Aldrich.
Ammonia (28%), toluene, acetone, and ethanol absolute were provided
by VWR CHEMICALS. N-Hexane (95%) was purchased from
Fisher Chemical. Ultrapure water was produced by Sartorius Arium 611
VF Water purification System with a resistivity of 18.2 MΩ·cm.
PS colloids with a diameter of 338 nm and 1.43 μm were prepared
using surfactant-free emulsion polymerization57 (link) and purified by several cycles of centrifugation and redispersion
in ultrapure water. Small and large particles therefore shared the
same surface chemistry.
× 76 mm in size
were provided by Menzel-Gläser, Germany. Tetraethoxysilane
(TEOS, reagent grade, 98%), Trichloro (1H, 1H, 2H, and 2H-perfluorooctyl)
silane (PFOTS, 97%), hexadecane (reagentPlus, 99%), and deuterium
oxide (D2O, 99.9 atom % D) were purchased from Aldrich.
Ammonia (28%), toluene, acetone, and ethanol absolute were provided
by VWR CHEMICALS. N-Hexane (95%) was purchased from
Fisher Chemical. Ultrapure water was produced by Sartorius Arium 611
VF Water purification System with a resistivity of 18.2 MΩ·cm.
PS colloids with a diameter of 338 nm and 1.43 μm were prepared
using surfactant-free emulsion polymerization57 (link) and purified by several cycles of centrifugation and redispersion
in ultrapure water. Small and large particles therefore shared the
same surface chemistry.
4
Enzymatic Analysis of Wheat Flour Carbohydrates
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All chemicals and solvents were HPLC-grade or higher. Water was deionized and purified using an Arium 611VF water purification system (Sartorius, Goettingen, Germany). Trypsin (from bovine pancreas, TPCKtreated, ≥ 10,000 BAEE U/mg protein), sodium hydroxide solution (50-52%; eluent for IC) and anhydrous sodium acetate (≥ 99%) were from Sigma-Aldrich (Steinheim, Germany). α-Amylase assay reagent (4-nitrophenyl α-dmaltoheptaoside (blocked), plus excess α-glucosidase and glucoamylase) and β-amylase assay reagent (4-nitrophenyl β-d-maltotrioside, plus excess thermostable β-glucosidase) were purchased from Megazyme (Bray, Ireland). d-( +)-Glucose (≥ 99.5%), d-(-)-fructose (≥ 99%), sucrose (≥ 99.5%), maltose monohydrate (≥ 99%), maltotriose (≥ 90%) and maltoheptaose (≥ 70%) were from Sigma-Aldrich; maltotetraose (≥ 97%), maltopentaose (≥ 90%), maltohexaose (≥ 95%) and maltooctaose (≥ 80%) from Santa Cruz (Dallas, USA). Wheat flour type 550, according to the German flour classification system, was provided by Rosenmühle (Ergolding, Germany).
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