Anti eif2a

Anti-GRP78, anti-eIF2a, anti-DNP, and anti-ATF6 antibodies were obtained from Santa Cruz Biotech (Santa Cruz, CA). Anti-cleaved caspase-3, anti-phospho-PERK, and anti-IRE1a antibodies were obtained from Cell Signaling Technology, Inc. (Beverly, MA). Anti-phospho-IRE1a (Ser724), anti-MANF, and anti-caspase-12 antibodies were obtained from Abcam (Cambridge, MA). Anti-X-box binding protein-1s (XBP1s) antibody was from BioLegend (San Diego, CA). 4-PBA and NAC were purchased from Sigma Chemical Co. (St. Louis, MO). 4-Hydroxynonenal (HNE) adduct assay was obtained from Cell Biolabs, Inc. (San Diego, CA). Small interfering (si) RNA for human MANF gene (si MANF) was purchased from invitrogen Co. (Carlsbad, CA). TUNEL Assay Kit was obtained from Abcam (Cambridge, MA). STF-083010 was obtained from Med Chem express (New Jersey, USA). Salubrinal was purchased from Tocris Bioscience (Avonmouth, Bristol, UK). Recombinant human MANF was obtained from Creative BioMart company (Shirley, NY). All other antibodies were purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA). Other chemicals/reagents used in this project were obtained from Thermo Fisher Scientific, Inc. (Waltham, MA, USA) unless stated otherwise.