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Goat anti lis1

Manufactured by Santa Cruz Biotechnology
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Goat anti-LIS1 is a primary antibody that recognizes the LIS1 (Lissencephaly-1) protein. LIS1 is a critical regulator of microtubule dynamics and is involved in various cellular processes, including neuronal migration and mitotic spindle orientation. This antibody can be used in techniques such as Western blotting, immunohistochemistry, and immunocytochemistry to detect and study the LIS1 protein.

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Lab products found in correlation

Normal goat serum (ngs), by Thermo Fisher Scientific (2 mentions) Poly l lysine coated coverslips, by BD (2 mentions) Dako wash buffer, by Agilent Technologies (2 mentions) Rabbit anti numb, by Abcam (2 mentions) Mouse anti alpha tubulin, by Abcam (2 mentions) Rat anti α tubulin, by Abcam (2 mentions) Mouse anti α tubulin conjugate fitc, by Merck Group (2 mentions) Tcs sp5 2 confocal, by Leica (2 mentions) Axio observer z1 microscope, by Leica (2 mentions) Lsm 700 scanning module, by Zeiss (2 mentions) Donkey serum, by Abcam (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Paraformaldehyde (pfa), by Thermo Fisher Scientific (2 mentions)

2 protocols using goat anti lis1

1

Immunocytochemistry of Cytoskeletal Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were allowed to settle on poly-L-lysine coated coverslips (BD Biosciences) at 37°C, fixed with 4% paraformaldehyde (USB Corporation) or methanol, permeabilized with 1X Dako wash buffer (Dako) and blocked with 20% normal goat serum (Invitrogen) or donkey serum (Abcam) in 1X Dako wash buffer. Primary antibody incubation was overnight at 4°C. The following primary antibodies were used: rabbit anti-Numb 1:50 or 1:100 (Abcam), goat anti-LIS1 1:500 (Santa Cruz Biotechnology), mouse anti-alpha-tubulin 1:200 (Abcam), rat anti-α-tubulin 1:1000 (Abcam), mouse anti-α-tubulin conjugate FITC 1:200 (Sigma). Secondary antibody incubation was performed for 1 hr at room temperature. DAPI (Molecular Probes) was used to detect DNA. Images were obtained with a Confocal Leica TCS SP5 II (Leica Microsystems) or an Axio Observer.Z1 microscope with the LSM 700 scanning module (Zeiss). ImageJ 1.46r was used to determine fluorescence intensity.
Zimdahl B., Ito T., Blevins A., Bajaj J., Konuma T., Weeks J., Koechlein C.S., Kwon H.Y., Arami O., Rizzieri D., Broome H.E., Chuah C., Oehler V.G., Sasik R., Hardiman G, & Reya T. (2014). Lis1 regulates asymmetric division in hematopoietic stem cells and in leukemia. Nature genetics, 46(3), 245-252.
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2

Immunocytochemistry of Cytoskeletal Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were allowed to settle on poly-L-lysine coated coverslips (BD Biosciences) at 37°C, fixed with 4% paraformaldehyde (USB Corporation) or methanol, permeabilized with 1X Dako wash buffer (Dako) and blocked with 20% normal goat serum (Invitrogen) or donkey serum (Abcam) in 1X Dako wash buffer. Primary antibody incubation was overnight at 4°C. The following primary antibodies were used: rabbit anti-Numb 1:50 or 1:100 (Abcam), goat anti-LIS1 1:500 (Santa Cruz Biotechnology), mouse anti-alpha-tubulin 1:200 (Abcam), rat anti-α-tubulin 1:1000 (Abcam), mouse anti-α-tubulin conjugate FITC 1:200 (Sigma). Secondary antibody incubation was performed for 1 hr at room temperature. DAPI (Molecular Probes) was used to detect DNA. Images were obtained with a Confocal Leica TCS SP5 II (Leica Microsystems) or an Axio Observer.Z1 microscope with the LSM 700 scanning module (Zeiss). ImageJ 1.46r was used to determine fluorescence intensity.
Zimdahl B., Ito T., Blevins A., Bajaj J., Konuma T., Weeks J., Koechlein C.S., Kwon H.Y., Arami O., Rizzieri D., Broome H.E., Chuah C., Oehler V.G., Sasik R., Hardiman G, & Reya T. (2014). Lis1 regulates asymmetric division in hematopoietic stem cells and in leukemia. Nature genetics, 46(3), 245-252.
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