All chemicals were purchased from MilliporeSigma (Burlington, MA) unless otherwise noted and were of the highest grade available. The Total Cholesterol and Cholesteryl Ester Colorimetric/Fluorometric Assay Kit was purchased from BioVision Inc (Milpitas, CA). Nile Red was purchased from Tokyo Chemical Industry (Tokyo, Japan). The Cyp46A1 antibody was purchased from GeneTex (Irvine, CA). The glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody was purchased from GeneTex (Irvine, CA). The primer for Cyp46A1 (catalog no: PPM03965A) and GAPDH (catalog no: PPM02946E) were purchased from Qiagen, SABiosciences (Frederick, MD). FXR vivo-morpholino sequences (FXR morpholino; 5′-CTGAAACTGCATCACCATCCTTAGC-3′), FXR mismatch vivo-morpholino sequences (FXR mismatch; 5′-CTCAAAGTGGATCACCATCGTTACC-3′), and Endo-Porter were purchased from Gene Tools (Philomath, OR). Guggulsterone was purchased from Tocris (Minneapolis, MN). Hematoxylin was purchased from Vector Laboratories (Burlingame, CA). Eosin was purchased from MilliporeSigma. Liver enzyme blood chemistry assays were purchased from IDEXX Laboratories, Inc (Westbrook, MA).
Glyceraldehyde 3 phosphate dehydrogenase gapdh antibody
Manufactured by GeneTex
Sourced in United States
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody is a protein that catalyzes a key step in glycolysis, the metabolic pathway that converts glucose into energy. This antibody can be used to detect and measure the presence of GAPDH in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Hematoxylin, by Vector Laboratories (1 mentions)
Total cholesterol and cholesteryl ester colorimetric fluorometric assay kit, by Abcam (1 mentions)
Nile red, by Tokyo Chemical Industry (1 mentions)
Guggulsterone, by Bio-Techne (1 mentions)
Gapdh, by Qiagen (1 mentions)
Endo porter, by Gene Tools (1 mentions)
Collagen solution from bovine skin, by Merck Group (1 mentions)
Rabbit polyclonal anti human antibody against col1a1 h 197, by Santa Cruz Biotechnology (1 mentions)
Picropodophyllin ppp, by Bio-Techne (1 mentions)
Alexa 488 conjugated anti mouse igg, by Jackson ImmunoResearch (1 mentions)
Arecoline, by Merck Group (1 mentions)
N acetylcysteine nac, by Bio-Techne (1 mentions)
β actin antibody, by Thermo Fisher Scientific (1 mentions)
Superscript 4 first strand synthesis system kit, by Thermo Fisher Scientific (1 mentions)
Hinokitiol, by Merck Group (1 mentions)
Pierce 1 step transfer buffer, by Thermo Fisher Scientific (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
Fast sybr green master mix, by Thermo Fisher Scientific (1 mentions)
Horseradish peroxidase hrp conjugated donkey anti rabbit immunoglobulin g igg, by Cytiva (1 mentions)
3 protocols using glyceraldehyde 3 phosphate dehydrogenase gapdh antibody
1
Cholesterol Metabolism Regulation Assay
2
Effects of Arecoline, NAC, and PPP on Cellular Markers
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Arecoline was purchased from Sigma–Aldrich (St. Louis, MO, USA). N-acetyl cysteine (NAC) and (picropodophyllin) PPP were purchased from Tocris Bioscience (Bristol, UK). Monoclonal mouse anti-human antibodies against α-SMA (1A4), vimentin (9E7E7), and ZEB1 (416A7H10) and rabbit polyclonal anti-human antibody against COL1A1 (H-197) and IGF-1Rβ (C-20) were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA). Rabbit polyclonal anti-human antibodies against p-IGF-1RTyr1161 and Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody was purchased from GeneTex Inc. (Hsinchu City, Taiwan). A collagen solution from bovine skin was purchased from Sigma–Aldrich. Horseradish peroxidase–conjugated antimouse IgG or anti-rabbit IgG antibodies were purchased from PerkinElmer (Waltham, MA, USA). Alexa 488–conjugated antimouse IgG and Alexa 594–conjugated anti-rabbit IgG antibodies were purchased from Jackson ImmunoResearch Laboratories Inc. (West Grove, PA, USA).
3
Hinokitiol Modulates Cell Signaling Pathways
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Hinokitiol (99%, Figure 1 A), dimethyl sulfoxide (DMSO), bovine serum albumin (BSA), collagenase type IV, and 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl tetrazolium bromide (MTT) were purchased from Sigma‐Aldrich (St. Louis, MO, USA). Type I collagen was purchased from Corning (NY, USA). The β‐actin antibody, Dulbecco's modified Eagle's medium (DMEM), L‐glutamine‐penicillin‐streptomycin, fetal bovine serum, Pierce™ 1‐Step Transfer Buffer, SuperScript™ IV First‐Strand Synthesis System Kit, and Fast SYBR™ Green Master Mix were purchased from Thermo Fisher (Waltham, MA, USA). The NucleoSpin® RNA kit was purchased from Macherey‐Nagel (Düren, Germany). Primary antibodies against Bax, Bcl‐2, phospho‐NF‐κB p65 (Ser536), IκBα, cleaved caspase‐3, and PARP were purchased from Cell Signaling (Beverly, MA, USA). The glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) antibody was purchased from GeneTex (Irvine, CA, USA). Hybond‐P polyvinylidene difluoride (PVDF) membrane, the enhanced chemiluminescence (ECL) western blotting detection reagent, horseradish peroxidase (HRP)‐conjugated donkey anti‐rabbit immunoglobulin G (IgG), and the sheep anti‐mouse IgG were purchased from Amersham (Buckinghamshire, UK). CF488A Donkey anti‐mouse IgG and CF594 Donkey anti‐rabbit IgG were purchased from Biotium (Fremont, CA, USA). Hinokitiol was dissolved in 0.1% dimethyl sulfoxide (DMSO) and stored at 4°C.
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