Log In Sign up
The largest database of trusted experimental protocols

Rabbit anti flag f7425

Manufactured by Merck Group
Visit Supplier

Rabbit anti-Flag (F7425) is a primary antibody produced in rabbits that specifically recognizes the Flag epitope tag. The core function of this product is to serve as a detection tool for proteins or other biomolecules that have been engineered to express the Flag tag.

Automatically generated - may contain errors

Lab products found in correlation

Anti mouse igg fitc, by Merck Group (2 mentions) Mouse anti flag f1804, by Merck Group (2 mentions) Rabbit anti ha h6908, by Merck Group (2 mentions) Mouse anti actin a1978, by Merck Group (2 mentions) Alexa fluor 647 conjugated ab190565, by Abcam (2 mentions) Mouse anti flag f3165, by Merck Group (2 mentions) Rabbit anti ha ab9110, by Abcam (1 mentions) Mouse anti α tubulin t5168, by Merck Group (1 mentions) Mouse anti ha h9658, by Merck Group (1 mentions) Sulforaphane sfn s8044, by LKT Laboratories (1 mentions) Rabbit anti gst 10000 0 ap, by Proteintech (1 mentions) Sc 003, by Invent Biotechnologies (1 mentions) Mouse anti myc, by Merck Group (1 mentions) Rabbit anti histone h4, by Active Motif (1 mentions) Rabbit anti ha, by Merck Group (1 mentions) Mouse anti flag m2, by Merck Group (1 mentions) Rabbit anti myc, by Merck Group (1 mentions) Mouse anti gapdh, by US Biological (1 mentions) Mouse anti control igg, by Santa Cruz Biotechnology (1 mentions) Anti mouse hrp linked, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Anti-FLAG (2121 citations) F7425 (190 citations) Rabbit anti-FLAG (183 citations) Anti-Flag (F7425, (35 citations) FLAG (F7425) (10 citations) Rabbit anti- (2 citations)

11 protocols using rabbit anti flag f7425

1

HA-tagged Protein Immunoprecipitation and Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed with lysis buffer (20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1 mM Na2EDTA, 1 mM EGTA, 1% TritonX-100, 2.5 mM sodium pyrophosphate, 1 mM -glycerophosphate, 1 mM Na3VO4) on ice for 15 min. Cell lysates were then centrifuged at 20,000 g for 30 min. The supernatant was immunoprecipitated with 20 µl of mouse anti-HA-agarose beads (A2095, Sigma) at 4 °C for 3 h or overnight. After the immunoprecipitation, the beads were washed four times in lysis buffer, and the immunoprecipitated proteins were subsequently eluted by 1× SDS sample buffer at room temperature for 10 min. The supernatant was collected and denatured at 100 °C for 2 min. The samples were subjected to western blotting with the corresponding antibodies. Rabbit anti-Flag (F7425, dilution 1:1000), mouse anti-α-tubulin (T5168, 1: 5000), and mouse anti-HA antibodies (H3663, dilution 1:1000) were purchased from Sigma. Rabbit anti-HA (ab9110, dilution 1:1000) and mouse anti-KAP1 (ab22553, dilution 1:1000) were from Abcam. The uncropped western blot images are provided in Supplementary Figure 6.
Chen W., Schwalie P.C., Pankevich E.V., Gubelmann C., Raghav S.K., Dainese R., Cassano M., Imbeault M., Jang S.M., Russeil J., Delessa T., Duc J., Trono D., Wolfrum C, & Deplancke B. (2019). ZFP30 promotes adipogenesis through the KAP1-mediated activation of a retrotransposon-derived Pparg2 enhancer. Nature Communications, 10, 1809.
+ Open protocol
+ Expand
2

Hsp90 Immunoprecipitation and Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols
HeLa cells were grown in 10-cm tissue culture dishes. Mock infections and infections were carried out according to a standard protocol. At 17 hpi, the cells were treated with DMSO or 300 nM geldanamycin for 1 h prior to harvesting. Cell lysates (1 ml) were incubated with 100 μl of a protein A-Sepharose bead slurry (P3391; Sigma-Aldrich) conjugated to isotype control (rabbit anti-FLAG [F7425; Sigma-Aldrich]) or Hsp90 antibody (rabbit anti-HSP90AB1 [11405-1-AP; Proteintech]). Lysate and beads were gently mixed overnight on ice. Subsequently, the mixture was centrifuged at 2,000 × g for 2 min at 4°C, and the supernatant was discarded. The beads were washed four times with lysis buffer. The beads were resuspended in 50 μl of 2× SDS sample buffer and heated at 100°C for 5 min, followed by centrifugation at 2,000 × g for 3 min. The proteins were separated by electrophoresis through SDS-PAGE gels, and Western blot analysis was carried out as described above.
Mohl B.P, & Roy P. (2019). Hsp90 Chaperones Bluetongue Virus Proteins and Prevents Proteasomal Degradation. Journal of Virology, 93(20), e00898-19.
+ Open protocol
+ Expand
3

Sulforaphane and Zidovudine Treatment Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Sulforaphane (SFN) (S8044) and Zidovudine (AZT) (L0350) were bought from LKT Laboratories. The mouse anti-actin (A1978), mouse anti-Flag (F1804), and mouse anti-HA (H9658) monoclonal antibodies; the rabbit anti-Flag (F7425), rabbit anti-HA (H6908), and anti-Mouse IgG-FITC antibodies were purchased from Sigma. Rat anti-Myc (ab206486), mouse anti-Tubulin (TA503129) monoclonal antibodies, Rabbit anti-Lamin (EPR8985), Alexa Fluor 568-conjugated (ab175476) and Alexa Fluor 647-conjugated (ab190565) antibodies were purchased from Abcam (Cambridge, UK). The rabbit anti-GST (10000-0-AP) antibodies were purchased from Proteintech. DyLight 800-labeled goat anti-mouse (5230–0415) and DyLight 508 680-labeled goat anti-rabbit (5230–0403) secondary antibodies were purchased from KPL. Anti-eqNrf2, anti-eqKeap1 and anti-P26 antibodies were prepared in our laboratory. The nuclear extraction kit was purchased from Invent Biotechnologies (SC-003). Non-cytotoxic dosages were used in this study.
Wang Y., Ma G., Wang X.F., Na L., Guo X., Zhang J., Liu C., Du C., Qi T., Lin Y, & Wang X. (2022). Keap1 recognizes EIAV early accessory protein Rev to promote antiviral defense. PLoS Pathogens, 18(2), e1009986.
+ Open protocol
+ Expand
4

Antibody Characterization for Molecular Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used: mouse anti-Flag (M2, Sigma-Aldrich, St. Louis, MO), rabbit anti-Flag (F7425, Sigma-Aldrich, St. Louis), mouse anti-RTA (mouse hybridoma), mouse anti-LANA (mouse hybridoma), mouse anti-Myc (mouse hybridoma), rabbit anti-HA (6908, Sigma-Aldrich, St. Louis, MO), mouse anti-HA12CA5 (sc-57592, Santa Cruz Biotechnology), mouse anti-GFP (G1546, Sigma-Aldrich, St. Louis), mouse anti-GAPDH (G8140, US Biological, Salem MA), and rabbit anti-Myc (SAB4300605, Sigma-Aldrich, St. Louis, MO), goat anti-PRMT5 (C-20, sc-22132, Santa Cruz Biotechnology), mouse anti-ORF59 (generous gift from Dr. Bala Chandran), rabbit anti-Control IgG (sc-2027, Santa Cruz Biotechnology), mouse anti-Control IgG (sc-2025, Santa Cruz Biotechnology), rabbit anti-H4R3me2s (61187, Active Motif, Carlsbad CA), rabbit anti-Histone H4 (#61299 Active Motif), rabbit-anti-PRMT5 antibody (#61001 Active Motif), rabbit anti-control IgG (ChIP grade—Cell Signaling Technology #2729, rabbit anti-COPR5 antibody (Novus Biologicals #NBP2-30884), and additional rabbit-anti ORF59 antibody custom synthesized for our lab by GenScript.
Strahan R.C., McDowell-Sargent M., Uppal T., Purushothaman P, & Verma S.C. (2017). KSHV encoded ORF59 modulates histone arginine methylation of the viral genome to promote viral reactivation. PLoS Pathogens, 13(7), e1006482.
+ Open protocol
+ Expand
5

Antibody Characterization for DNA Damage Response

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies were as follows: rabbit anti-FANCD2 (NB100-182; Novus) at 1:10,000; rabbit anti-FANCI (A301-254A; Bethyl) at 1:1,000; rabbit anti-SF3B1 (A300-996A; Bethyl) at 1:2,000; rabbit anti-SF3B1 (Eurogentec) at 1:1,000; rabbit anti-SF3A3 (A302-507A; Bethyl) at 1:2,000; rabbit anti-FLAG (F7425; Sigma-Aldrich) at 1:1,000; rabbit anti-DHX15 (A300-389A; Bethyl) at 1:5,000, mouse anti-SC35 (556363; BD Pharmingen) at 1:500; rabbit anti-U2AF65 (ab37530; Abcam) at 1:250; rabbit anti-pSer345(Chk1) (2348L, rabbit; Cell Signaling Technology) at 1:1,000; mouse anti-Chk1 (Sc-8408; Santa Cruz Biotechnology) at 1:1,000; mouse anti-tubulin (T5168; Sigma-Aldrich) at 1:10,000; anti-rabbit HRP-linked (7074; Cell Signaling Technology) at 1:2,500; anti-mouse HRP-linked (7076; Cell Signaling Technology) at 1:2,500; rabbit anti-Nbs1 (NB100-143; Novus Biologicals) at 1:1,000; mouse anti-PCNA (P8825; Sigma-Aldrich) at 1:3,000; rabbit anti-TopBP1 (A300-111A; Bethyl) at 1:1,000; rabbit anti-RNA-PolII (N-20) (sc-899; Santa Cruz Biotechnology) at 1:1,000; rabbit anti-TFIP11 (A302-548A; Bethyl) at 1:1,000; rabbit anti-SPF45 (A302-548A; Bethyl) at 1:1,000; rabbit anti-FANCA (A301-980A; Bethyl) at 1:2,000; and rabbit anti-PRP8 (GTX108046; GeneTex) at 1:1,000.
Moriel-Carretero M., Ovejero S., Gérus-Durand M., Vryzas D, & Constantinou A. (2017). Fanconi anemia FANCD2 and FANCI proteins regulate the nuclear dynamics of splicing factors. The Journal of Cell Biology, 216(12), 4007-4026.
+ Open protocol
+ Expand
6

Antibody Identification for Biomarker Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse anti-BRCA1 (MS110; OP92), mouse anti-R-loop (S9.6; MABE1095), mouse anti-dsDNA (AE-2; MAB1293), and mouse anti-dsRNA (rJ2; MABE1134) were purchased from EMD Millipore. Mouse anti-BRCA2 mAb (OP95) was purchased from Calbiochem. Rabbit anti-nucleolin (D4C70; 14574), rabbit anti-β-actin (13E5; 4970), and rabbit anti-β-tubulin (9F3; 2128) were purchased from Cell Signaling Technology. Mouse anti-GAPDH (AC002) was purchased from ABClonal. Rabbit anti-FLAG (F7425) was purchased from Sigma-Aldrich.
Chang C.W., Singh A.K., Li M., Guan L., Le N., Omabe K., Liang F, & Liu Y. (2022). The BRCA1 BRCT promotes antisense RNA production and double-stranded RNA formation to suppress ribosomal R-loops. Proceedings of the National Academy of Sciences of the United States of America, 119(50), e2217542119.
+ Open protocol
+ Expand
7

Antibody Specifications for Protein Interactions

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following are the specificities and sources of antibodies used: Against phosphotyrosine (PY20; GE Healthcare), the FLAG epitope (mouse monoclonal M2, F3165, and rabbit anti-FLAG, F7425; Sigma), the Myc epitope (rabbit anti-Myc, 600–401–381; Rockland, and mouse monoclonal 9E10; Sigma), GFP (mouse monoclonal anti-GFP, 11–814–460–001; Roche), Hic-5 (mouse monoclonal anti-Hic-5, 611164; BD Biosciences), paxillin (mouse monoclonal anti-paxillin, 610569; BD Biosciences, and rabbit anti-paxillin, sc-5574; Santa Cruz Biotechnology), and Git1 (rabbit anti-Git1, sc-13961; Santa Cruz Biotechnology, and mouse anti-Git1 monoclonal antibody, 611396; BD Biosciences). Rabbit antisera specific for the amino acid residues 251–555 of Git1 (anti-GIT1/Cat-1) [13 (link)], rabbit polyclonal antibodies against phospho-Tyr-554 on Git1 (anti-pY554-Git1) [12 (link)], and a rabbit anti-Ptprz-S serum [15 (link)] were prepared in our laboratory. Anti-pY554-Git1 antibodies were conjugated with horseradish peroxidase (HRP) using a peroxidase labeling kit (Dojindo Molecular Technologies).
Fujikawa A., Matsumoto M., Kuboyama K., Suzuki R, & Noda M. (2015). Specific Dephosphorylation at Tyr-554 of Git1 by Ptprz Promotes Its Association with Paxillin and Hic-5. PLoS ONE, 10(3), e0119361.
+ Open protocol
+ Expand
8

Antibody and RNA Synthesis Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
The mouse anti-actin (A1978), mouse anti-FLAG (F1804), and mouse anti-HA (H9658) monoclonal antibodies and the rabbit anti-FLAG (F7425), rabbit anti-HA (H6908), and anti-Mouse IgG-FITC antibodies were purchased from Sigma. Mouse anti-tubulin (TA503129) monoclonal antibodies, rabbit anti-lamin (EPR8985), Alexa Fluor 568-conjugated (ab175476), and Alexa Fluor 647-conjugated (ab190565) antibodies were purchased from Abcam (Cambridge, UK). DyLight 800-labeled goat anti-mouse (5230-0415) and DyLight 680-labeled goat anti-rabbit (5230-0403) secondary antibodies were purchased from KPL. Anti-Grev and anti-p26 antibodies were prepared in our laboratory. The T7 quick high-yield RNA synthesis kit (E2050S) was purchased from NEB. The RNA 3′-end desthiobiotinylation kit (20163) and streptavidin magnetic beads (88816) were purchased from Thermo. Leptomycin B (LMB) was purchased from Beyotime Biotechnology.
Li J., Zhang X., Bai B., Zhang M., Ma W., Lin Y., Wang X, & Wang X.F. (2022). Identification of a Novel Post-transcriptional Transactivator from the Equine Infectious Anemia Virus. Journal of Virology, 96(24), e01210-22.
+ Open protocol
+ Expand
9

Antibody Validation for Protein Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse anti-Myc (9E10), rabbit anti-HA (F-7), mouse anti-α-tubulin (B-7) and mouse anti-β-tubulin (3F3-G2) antibodies were purchased from Santa Cruz. Mouse anti-Flag (F3165), rabbit anti-Flag (F7425) and mouse anti-ubiquitin (P4D1) antibodies were purchased from Sigma-Aldrich. Rabbit anti-cGAS (D1D3G), rabbit anti-STING (D2P2F) and rabbit anti-TBK1 (D1B4) antibodies were from Cell Signaling Technology. Mouse anti-V5 (E10/V4RR) antibodies were from Invitrogen.
Lui W.Y., Bharti A., Wong N.H., Jangra S., Botelho M.G., Yuen K.S, & Jin D.Y. (2023). Suppression of cGAS- and RIG-I-mediated innate immune signaling by Epstein-Barr virus deubiquitinase BPLF1. PLOS Pathogens, 19(2), e1011186.
+ Open protocol
+ Expand
10

Antibody Characterization for DNA Damage Response

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies used in this study were as follows: anti-USP15 (A300-923A, WB: 1:1000), anti-BARD1 (A300-263A, WB: 1:1000; IF: 1:200), and anti-RAP80 (A300-764A, WB: 1:1000; IF: 1:100) were purchased from Bethyl Laboratory. Anti-HP1γ (MABE656, WB: 1:2000). Mouse anti-γ-H2AX (05-636, IF: 1:500), mouse anti-MDC1 (05-1572, IF: 1:300) and mouse anti-FK2 (04-263, IF: 1:200) were purchased from Millipore. Anti-RPA 70 (#2267, IF: 1:200), rabbit anti-γ-H2AX (#9718, IF: 1:400), and rabbit anti-HA (#3724, WB: 1:2500; IF: 1:300) were from Cell signaling Technology. Anti-Rad51 (GTX100469, IF: 1:200) were from Genetex. Anti-BRCA1 (D9, IF: 1:50) and anti-His tag (sc8036, WB: 1:1000) were from Santa Cruz. Anti-poly PAR (4336-BPC-100, WB: 1:1000) were from Trevigon. Anti-53BP1 (NB100-304, WB: 1:1000; IF: 1:300) were from Novus Biologicals. Anti-RNF8 (14112-1-AP, IF: 1:100) were from Proteintech. Rabbit anti-FLAG (F7425, WB: 1:2500) and mouse anti-FLAG (F3165, WB: 1:2500) were from Sigma-Aldrich. Mouse anti-HA (901501, WB: 1:2500; IF: 1:100) were from BioLegend.
Peng Y., Liao Q., Tan W., Peng C., Hu Z., Chen Y., Li Z., Li J., Zhen B., Zhu W., Li X., Yao Y., Song Q., Liu C., Qi X., He F, & Pei H. (2019). The deubiquitylating enzyme USP15 regulates homologous recombination repair and cancer cell response to PARP inhibitors. Nature Communications, 10, 1224.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!