Continuous gel electrophoresis

Manufactured by Bio-Rad

Continuous gel electrophoresis is a laboratory technique used to separate and analyze biomolecules, such as proteins or nucleic acids, based on their size and charge. The equipment consists of a gel-filled chamber with electrodes at each end, allowing for the continuous flow of an electric current through the sample. This process enables the separation and visualization of the target biomolecules.

Automatically generated - may contain errors

Lab products found in correlation

Silver express kit, by Thermo Fisher Scientific (2 mentions) Gradient gel, by Bio-Rad (2 mentions)

2 protocols using continuous gel electrophoresis

Purification and Characterization of Recombinant Tau Oligomers

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Example 6

Recombinant human Tau412 protein was expressed in bacteria using standard methods and purified using cation exchange with SP Sepharose and size fractionation using continuous gel electrophoresis (BioRad). The protein was incubated at 1 mg/ml in 50 mM Tris-HCl pH 7.4 and 1 mg of the oligomerized preparation was used for purification of individual oligomers using continuous gel electrophoresis (BioRad). Non-reducing sample buffer was used to prepare samples for SDS-PAGE using a 4-20% gradient gel (BioRad) to resolve purified tau monomer, dimer, trimer and tetramer. Silver staining was performed with the Silver Express kit (Invitrogen) (shown in FIG. 11).

US11104710B2. Methods and compositions comprising tau oligomers (2021-08-31). OLIGOMERIX, INC. [US]. Inventors: James G. Moe [US], Eliot J. Davidowitz [US].

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Purification and Characterization of Recombinant Human Tau441

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Example 5

Recombinant human Tau441 protein was expressed in bacteria using standard methods and purified using cation exchange with SP Sepharose and size fractionation using continuous gel electrophoresis (BioRad). The protein was incubated at 1 mg/ml in 50 mM Tris-Hcl pH 7.4. SDS-PAGE using a 4-20% gradient gel (BioRad) was used to resolve the oligomeric species. Silver staining was performed with the Silver Express kit (Invitrogen) (shown in FIG. 10).

US11104710B2. Methods and compositions comprising tau oligomers (2021-08-31). OLIGOMERIX, INC. [US]. Inventors: James G. Moe [US], Eliot J. Davidowitz [US].

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