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Anti cd45 percp

Manufactured by Beckman Coulter
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The Anti-CD45-PerCp is a monoclonal antibody conjugated with the fluorescent dye PerCp (Peridinin Chlorophyll Protein). It is designed to specifically bind to the CD45 antigen, which is expressed on the surface of all human leukocytes. The fluorescent conjugate allows for the identification and enumeration of CD45-positive cells in flow cytometry applications.

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Lab products found in correlation

Anti cd71 fitc, by Beckman Coulter (7 mentions) Cd34 apc, by Beckman Coulter (7 mentions) Anti cd41 fitc gpiib iiia, by BD (7 mentions) Anti cd33 fitc, by Beckman Coulter (7 mentions) Anti cd41 pe, by Beckman Coulter (7 mentions) Facscalibur, by BD (7 mentions)

Spelling variants of this product

Anti-CD45 (9 citations)

7 protocols using anti cd45 percp

1

Multiparametric Analysis of Platelet Differentiation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 34

The differentiation state of platelets in culture can be assessed by flow cytometry. Megakaryocytes (MKs) represent a distinct cellular morphology that precedes terminal platelet differentiation. To determine the extent of maturation toward MKs, 1×10^6 cultured cells (LAMA-84 and CD34+ cells) are washed and then labeled with (a) anti-CD41-FITC (GpIIb/IIIa; BD Bioscience, San Jose, Calif., USA) or anti CD71-FITC or (b) anti-CD33-FITC, anti-CD41-PE, anti-CD45-PerCp and CD34-APC (Beckman Coulter, Fullerton, Calif., USA), and analyzed for the percentage of CD41 cells generated.

To determine the amount of ploidy, differentiated LAMA-84 cells are fixed overnight in 75% ethanol at 4° C. and labeled with propidium iodide (PI, 50 μg/ml) and analyzed using the FACScalibur (Becton Dickinson), whereas day 14 differentiated CD34+ cells are analyzed quantitatively under a microscope after May-Grunwald/Giemsa staining by quantitating the number of nuclei per cell and specific morphology of MKs with this stain. Only cells with MK morphology are analyzed. The presence of multinucleated cells in the cytospin preparation is indicative of the presence of polyploid MKs. Differentiated CD34+ cells are assessed for the presence of multinucleated mature MKs by morphology.

US10253296B2. Synthetic membrane-receiver complexes (2019-04-09). RUBIUS THERAPEUTICS, INC. [US]. Inventors: Avak Kahvejian [US], Jordi Mata-Fink [US], John Round [US], David Arthur Berry [US], Noubar B. Afeyan [US].
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2

Assessing Platelet Differentiation by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 44

The differentiation state of platelets in culture can be assessed by flow cytometry. Megakaryocytes (MKs) represent a distinct cellular morphology that precedes terminal platelet differentiation. To determine the extent of maturation toward MKs, 1×10{circumflex over ( )}6 cultured cells (LAMA-84 and CD34+ cells) are washed and then labeled with (a) anti-CD41-FITC (GpIIb/IIIa; BD Bioscience, San Jose, Calif., USA) or anti CD71-FITC or (b) anti-CD33-FITC, anti-CD41-PE, anti-CD45-PerCp and CD34-APC (Beckman Coulter, Fullerton, Calif., USA), and analyzed for the percentage of CD41 cells generated.

To determine the amount of ploidy, differentiated LAMA-84 cells are fixed overnight in 75% ethanol at 4° C. and labeled with propidium iodide (PI, 50 ug/ml) and analyzed using the FACScalibur (Becton Dickinson), whereas day 14 differentiated CD34+ cells are analyzed quantitatively under a microscope after May-Grunwald/Giemsa staining by quantitating the number of nuclei per cell and specific morphology of MKs with this stain. Only cells with MK morphology are analyzed. The presence of multinucleated cells in the cytospin preparation is indicative of the presence of polyploid MKs. Differentiated CD34+ cells are assessed for the presence of multinucleated mature MKs by morphology.

US11554141B2. Methods and compositions for immunomodulation (2023-01-17). RUBIUS THERAPEUTICS, INC. [US]. Inventors: Jordi Mata-Fink [US], John Round [US], Noubar B. Afeyan [US], Avak Kahvejian [US].
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3

Assessing Platelet Differentiation by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 34

The differentiation state of platelets in culture can be assessed by flow cytometry. Megakaryocytes (MKs) represent a distinct cellular morphology that precedes terminal platelet differentiation. To determine the extent of maturation toward MKs, 1×10{circumflex over ( )}6 cultured cells (LAMA-84 and CD34+ cells) are washed and then labeled with (a) anti-CD41-FITC (GpIIb/IIIa; BD Bioscience, San Jose, Calif., USA) or anti CD71-FITC or (b) anti-CD33-FITC, anti-CD41-PE, anti-CD45-PerCp and CD34-APC (Beckman Coulter, Fullerton, Calif., USA), and analyzed for the percentage of CD41 cells generated.

To determine the amount of ploidy, differentiated LAMA-84 cells are fixed overnight in 75% ethanol at 4° C. and labeled with propidium iodide (PI, 50 μg/ml) and analyzed using the FACScalibur (Becton Dickinson), whereas day 14 differentiated CD34+ cells are analyzed quantitatively under a microscope after May-Grunwald/Giemsa staining by quantitating the number of nuclei per cell and specific morphology of MKs with this stain. Only cells with MK morphology are analyzed. The presence of multinucleated cells in the cytospin preparation is indicative of the presence of polyploid MKs. Differentiated CD34+ cells are assessed for the presence of multinucleated mature MKs by morphology.

US10557119B2. Erythroid cells comprising phenylalanine ammonia lyase (2020-02-11). RUBIUS THERAPEUTICS, INC. [US]. Inventors: Avak Kahvejian [US], Jordi Mata-Fink [US], John Round [US], David Arthur Berry [US], Noubar B. Afeyan [US].
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4

Assessing Platelet Differentiation by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 34

The differentiation state of platelets in culture can be assessed by flow cytometry. Megakaryocytes (MKs) represent a distinct cellular morphology that precedes terminal platelet differentiation. To determine the extent of maturation toward MKs, 1×10{circumflex over ( )}6 cultured cells (LAMA-84 and CD34+ cells) are washed and then labeled with (a) anti-CD41-FITC (GpIIb/IIIa; BD Bioscience, San Jose, Calif., USA) or anti CD71-FITC or (b) anti-CD33-FITC, anti-CD41-PE, anti-CD45-PerCp and CD34-APC (Beckman Coulter, Fullerton, Calif., USA), and analyzed for the percentage of CD41 cells generated.

To determine the amount of ploidy, differentiated LAMA-84 cells are fixed overnight in 75% ethanol at 4° C. and labeled with propidium iodide (PI, 50 μg/ml) and analyzed using the FACScalibur (Becton Dickinson), whereas day 14 differentiated CD34+ cells are analyzed quantitatively under a microscope after May-Grunwald/Giemsa staining by quantitating the number of nuclei per cell and specific morphology of MKs with this stain. Only cells with MK morphology are analyzed. The presence of multinucleated cells in the cytospin preparation is indicative of the presence of polyploid MKs. Differentiated CD34+ cells are assessed for the presence of multinucleated mature MKs by morphology.

US10344263B2. Synthetic membrane-receiver complexes (2019-07-09). RUBIUS THERAPEUTICS, INC. [US]. Inventors: Avak Kahvejian [US], Jordi Mata-Fink [US], John Round [US], David Arthur Berry [US], Noubar B. Afeyan [US].
+ Open protocol
+ Expand
5

Multiparametric Analysis of Platelet Differentiation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 34

The differentiation state of platelets in culture can be assessed by flow cytometry. Megakaryocytes (MKs) represent a distinct cellular morphology that precedes terminal platelet differentiation. To determine the extent of maturation toward MKs, 1×10^6 cultured cells (LAMA-84 and CD34+ cells) are washed and then labeled with (a) anti-CD41-FITC (GpIIb/IIIa; BD Bioscience, San Jose, Calif., USA) or anti CD71-FITC or (b) anti-CD33-FITC, anti-CD41-PE, anti-CD45-PerCp and CD34-APC (Beckman Coulter, Fullerton, Calif., USA), and analyzed for the percentage of CD41 cells generated.

To determine the amount of ploidy, differentiated LAMA-84 cells are fixed overnight in 75% ethanol at 4° C. and labeled with propidium iodide (PI, 50 μg/ml) and analyzed using the FACScalibur (Becton Dickinson), whereas day 14 differentiated CD34+ cells are analyzed quantitatively under a microscope after May-Grunwald/Giemsa staining by quantitating the number of nuclei per cell and specific morphology of MKs with this stain. Only cells with MK morphology are analyzed. The presence of multinucleated cells in the cytospin preparation is indicative of the presence of polyploid MKs. Differentiated CD34+ cells are assessed for the presence of multinucleated mature MKs by morphology.

US10301594B1. Synthetic membrane-receiver complexes (2019-05-28). RUBIUS THERAPEUTICS, INC. [US]. Inventors: Avak Kahvejian [US], Jordi Mata-Fink [US], John Round [US], David Arthur Berry [US], Noubar B. Afeyan [US].
+ Open protocol
+ Expand
6

Platelet Differentiation Analysis by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 44

The differentiation state of platelets in culture can be assessed by flow cytometry. Megakaryocytes (MKs) represent a distinct cellular morphology that precedes terminal platelet differentiation. To determine the extent of maturation toward MKs, 1×10{circumflex over ( )}6 cultured cells (LAMA-84 and CD34+ cells) are washed and then labeled with (a) anti-CD41-FITC (GpIIb/IIIa; BD Bioscience, San Jose, Calif., USA) or anti CD71-FITC or (b) anti-CD33-FITC, anti-CD41-PE, anti-CD45-PerCp and CD34-APC (Beckman Coulter, Fullerton, Calif., USA), and analyzed for the percentage of CD41 cells generated.

To determine the amount of ploidy, differentiated LAMA-84 cells are fixed overnight in 75% ethanol at 4° C. and labeled with propidium iodide (PI, 50 μg/ml) and analyzed using the FACScalibur (Becton Dickinson), whereas day 14 differentiated CD34+ cells are analyzed quantitatively under a microscope after May-Grunwald/Giemsa staining by quantitating the number of nuclei per cell and specific morphology of MKs with this stain. Only cells with MK morphology are analyzed. The presence of multinucleated cells in the cytospin preparation is indicative of the presence of polyploid MKs. Differentiated CD34+ cells are assessed for the presence of multinucleated mature MKs by morphology.

US11576934B2. Methods and compositions for immunomodulation (2023-02-14). RUBIUS THERAPEUTICS, INC. [US]. Inventors: Jordi Mata-Fink [US], John Round [US], Noubar B. Afeyan [US], Avak Kahvejian [US].
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7

Assessing Platelet Differentiation and Maturation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 34

The differentiation state of platelets in culture can be assessed by flow cytometry. Megakaryocytes (MKs) represent a distinct cellular morphology that precedes terminal platelet differentiation. To determine the extent of maturation toward MKs, 1×10^6 cultured cells (LAMA-84 and CD34+ cells) are washed and then labeled with (a) anti-CD41-FITC (GpIIb/IIIa; BD Bioscience, San Jose, Calif., USA) or anti CD71-FITC or (b) anti-CD33-FITC, anti-CD41-PE, anti-CD45-PerCp and CD34-APC (Beckman Coulter, Fullerton, Calif., USA), and analyzed for the percentage of CD41 cells generated.

To determine the amount of ploidy, differentiated LAMA-84 cells are fixed overnight in 75% ethanol at 4° C. and labeled with propidium iodide (PI, 50 μg/ml) and analyzed using the FACScalibur (Becton Dickinson), whereas day 14 differentiated CD34+ cells are analyzed quantitatively under a microscope after May-Grunwald/Giemsa staining by quantitating the number of nuclei per cell and specific morphology of MKs with this stain. Only cells with MK morphology are analyzed. The presence of multinucleated cells in the cytospin preparation is indicative of the presence of polyploid MKs. Differentiated CD34+ cells are assessed for the presence of multinucleated mature MKs by morphology.

US10329531B2. Synthetic membrane-receiver complexes (2019-06-25). RUBIUS THERAPEUTICS, INC. [US]. Inventors: Avak Kahvejian [US], Jordi Mata-Fink [US], John Round [US], David Arthur Berry [US], Noubar B. Afeyan [US].
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