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Sirna asic1

Manufactured by Merck Group
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SiRNA ASIC1 is a laboratory equipment product developed by Merck Group. It is designed for the delivery and study of small interfering RNA (siRNA) targeting the Acid-Sensing Ion Channel 1 (ASIC1) gene. The core function of this product is to enable researchers to investigate the effects of ASIC1 gene silencing in various experimental settings.

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Lab products found in correlation

Sirna asic3, by Merck Group (2 mentions) I fect, by Neuromics (2 mentions)

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SiRNAs

2 protocols using sirna asic1

1

Intrathecal Targeting of ASIC Channels

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In the rodent model of RE, PcTx1, APTEx‐2, or vehicle (saline) (1 nmol per rat) was administered intrathecally 15 min before the ED test.24, 25 In separate studies, 10 ml of a siRNA (ASIC1 or ASIC3) (2 μg)/i‐Fect (Neuromics) mix was injected intrathecally using a Hamilton syringe and a 25‐gauge needle. Animals received one injection per day for 3 days before the ED test. SiRNA ASIC1, SiRNA ASIC3 and the corresponding scramble siRNAs were ordered from Sigma. Intrathecal injections were performed freehand under isoflurane anesthesia (2% isoflurane inhalation) between spinal T3 and T5 vertebrae of rats as previously described.26 RT‐PCR studies showed that intrathecal administration of specific ASIC1 and ASIC3 siRNAs resulted in >70% reduction of ASIC1 and 3 expression in the T3‐T5 thoracic dorsal root ganglia.
Han X., Zhang Y., Lee A., Li Z., Gao J., Wu X., Zhao J., Wang H., Chen D., Zou D, & Owyang C. (2021). Upregulation of acid sensing ion channels is associated with esophageal hypersensitivity in GERD. The FASEB Journal, 36(1), e22083.
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2

Intrathecal Modulation of ASIC Receptors in Rodent RE

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In the rodent model of RE, PcTx1, APTEx-2, or vehicle (saline) (1nmol per rat) was administered intrathecally 15 min before the esophageal distension test.24 ,25 (link) In separate studies, ten microliters of a siRNA (ASIC1 or ASIC3) (2ug)/i-Fect (Neuromics) mix was injected intrathecally using a Hamilton syringe and a 25-gauge needle. Animals received one injection per day for 3 days before the esophageal distension test. SiRNA ASIC1, SiRNA ASIC3 and the corresponding scramble siRNAs were ordered from Sigma. Intrathecal injections were performed freehand under isoflurane anesthesia (2% isoflurane inhalation) between spinal T3 and T5 vertebrae of rats as previously described.26 (link) RT-PCR studies showed that intrathecal administration of specific ASIC1 and ASIC3 siRNAs resulted in >70% reduction of ASIC1 and 3 expression in the T-3-T5 thoracic dorsal root ganglia.
Han X., Zhang Y., Lee A., Li Z., Gao J., Wu X., Zhao J., Wang H., Chen D., Zou D, & Owyang C. (2021). Upregulation of acid sensing ion channels is associated with esophageal hypersensitivity in GERD. The FASEB Journal, 36(1), e22083.
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