Uv mini 1240 pc

The lipid oxidation rate was measured using 2-TBARS assays to quantify the malondialdehyde (MDA). In brief, cooked breast samples (0.5 g) were prepared in triplicate in a 25 mL TBARS test tube, added with an antioxidant mixture (0.1 mL), 1% TBA in 0.3% NaOH (3 mL), and assigned into a thorough vortex for 30 s. The 17 mL of 2.5% trichloroacetic acid in 36 mM HCl was added, sealed, and heated in a water bath (BW-20G, Biotechnical Services, North Little Rock, AR, USA) at 100°C for 30 min. The tubes were immersed into ice water for 10 min once heating was completed. Each of 5 mL of the aqueous sample was taken into a new 15 mL conical tube, mixed with 3 mL of chloroform, and centrifuged at 2,400×g for 30 min at 4°C (1248R, LaboGene, Lillerød, Denmark). The absorbance was recorded at 532 nm using a UV spectrophotometer (UV-mini 1240 PC, Shimadzu, Kyoto, Japan) and compared against a blank.

The 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays were performed to measure the antioxidant activity of the cooked breast samples based on the protocol by Islam et al. (2016) (link). The extract solution (100 μL) was placed in 100 μL of methanolic solution containing DPPH radicals (0.2 mM). The mixture was allowed to react for 30 min at 25°C in the dark. The absorbance of each extract solution was measured at 517 nm using a spectrophotometer (UV-mini 1240 PC, Shimadzu). The results were expressed as a scavenging percentage of free radicals.

Lipid oxidation was analyzed using the TBARS method of Sinhuber and Yu [22 (link)], with modifications. Samples (0.5
g) were prepared in triplicate and vortex-mixed with 3 mL of 1% TBA in 0.3%
NaOH and 17 mL of 2.5% trichloroacetic acid in 36 mM HCl (Sigma-Aldrich, St.
Louis, MO). The samples were heated in a water bath (BW-20 G, Biotechnical
Services, San Diego, CA, USA) at 98°C for 30 min and then immersed in
ice water for 10 min. Subsequently, 5 mL of an aqueous sample was
centrifuged at 2,800×g for 30 min at 4°C (1248R, Labogene,
Lillerød, Denmark). The absorbance of the clear pink layer was
recorded at 532 nm (UV Mini 1240 PC, Shimadzu, Kyoto, Japan) against the
blank. The result was expressed in milligrams of malondialdehyde (MDA) per
kg of meat.

The measurement of lipid oxidation was performed using 2-thiobarbituric acid
reactive substances (TBARS). A sample of 0.5 grams in a 25-mL TBARS test tube
was prepared with three repetitions, and 0.1 g of antioxidant mixture
(consisting of 54% propylene glycol, 40% Tween 20, 3%
butylated hydroxytoluene and 3% butylated hydroxyanisole) was transferred
to the tube. Subsequently, 3 mL of 1% thiobarbituric acid in 0.3%
NaOH was added to the mixture. Immediately after vortexing, 17 mL of 2.5%
trichloroacetic acid in 36 mM HCl was added, and the tube was closed. The sample
was subjected to heating in a water bath (BW-20G, Biotechnical Services, North
Little Rock, AR, USA) at a temperature of 100°C for 30 min. The tube was
then immersed in cold water for another 15 min. A 5 mL aqueous sample was
transferred to a new 15 mL centrifuge tube and mixed with 3 mL of chloroform.
The mixture was then subjected to centrifugation at 2,400×g for 30 min at
4°C (1248R, Labogene, Lynge, Denmark) to separate it from the pellet. The
absorbance was measured at 532 nm by using a UV-spectrophotometer (UV-mini 1240
PC, Shimadzu, Kyoto, Japan) against a blank (distilled water was used to replace
the sample). Each sample was repeated three times. Data are expressed in the
form of mg malondialdehyde/kg sample.

The 2-thiobarbituric acid reactive substances (TBARS) method was used to quantify the malondialdehyde concentration at the same day following treatments. Briefly, after 30 min resting in the chill room at 4 ± 2°C chicken breast samples (0.5 g) were prepared in triplicate in a 25-mL TBARS test tube, with the addition of antioxidant mixture (0.1 g). Three milliliters of 1% TBA in 0.3% NaOH was added to the mixture, it was then vortexed, followed by the addition of 17 mL of 2.5% trichloroacetic acid in 36 mM HCl. After the tubes were sealed, they were heated in a water bath (BW-20G, Biotechnical Services, North Little Rock, AR) at a temperature of 100°C for 30 min. The tubes were directly immersed in ice water once heating was completed. Every 5 mL of aqueous sample was moved to a new 15 mL conical tube and mixed with 3 mL of chloroform. The mixtures were subsequently subjected to centrifugation at 2,400 × g for 30 min at 4°C (1248R, Labogene, Lynge, Denmark), and the absorbance was recorded at 532 nm using a UV spectrophotometer (UV-mini 1240 PC, Shimadzu, Kyoto, Japan) and compared against a blank.

Lipid oxidation was analyzed using the 2-thiobarbituric acid reactive substances (TBARS) method by Sinnhuber and Yu [12 ] with slight modification. A total of 0.5 g of sample was vortex-mixed with 0.1 g of antioxidant mixture (consisting of 54% propylene glycol, 40% Tween 20, 3% butylated hydroxytoluene, and 3% butylated hydroxyanisole), 3 mL of 1% TBA in 0.3% NaOH, and 17 mL of 2.5% trichloroacetic acid in 36 mM HCl. The sample was heated in a water bath (BW-20G, Biotechnical Services, Inc., North Little Rock, AR, USA) at 100°C for 30 min and then immersed in ice water for 15 min. Subsequently, 5 mL of aqueous sample was mixed with 3 mL of chloroform. The absorbance value of the upper layer was recorded at 532 nm (UV Mini 1240 PC, Shimadzu Corp., Japan) against blank after centrifuging at 2,400×g for 30 min at 4°C (1248R, Labogene, Lynge, Denmark). The result was expressed in mg of malondialdehyde (MDA) per kg of meat. Each sample was analyzed in triplicate.