To identify the differentially expressed genes upon PTBP1 knockout, the total RNA was isolated from the PTBP1 knockout or control MKN28 cells using TRIzol reagent, and PolyA RNA was subsequently purified from the total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module. RNA-seq was performed to detect the mRNA expression profiles at GENTED (Shanghai, China) using HiSeq3000 (Illumina, USA). The differentially expressed genes with a fold change > 2 and a P-value < 0.05 were selected. To reveal the PTBP1-bound mRNAs, RIP experiments were conducted using a PTBP1 antibody (Cell Signaling Technology) or IgG. The total RNA was isolated with TRIzol (Invitrogen), and ribosomal RNA was removed from the total RNA. RNA-seq was performed at CLOUDSEQ (Shanghai, China) using HiSeq3000 (Illumina, USA). The data are available via GEO under accession numbers GSE157582 and GSE157941.
Ptbp1 antibody
Manufactured by Cell Signaling Technology
The PTBP1 antibody is a laboratory reagent used to detect and study the PTBP1 protein in biological samples. PTBP1, also known as polypyrimidine tract-binding protein 1, is a regulatory protein involved in various cellular processes, including RNA splicing and translation. This antibody can be used in techniques such as Western blotting, immunoprecipitation, and immunocytochemistry to identify and analyze the PTBP1 protein.
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Lab products found in correlation
2 protocols using ptbp1 antibody
1
PTBP1 Knockout Transcriptome Profiling
2
PTBP1 Knockout Transcriptome and Binding
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To seek out differentially expressed genes upon PTBP1 knockout, total RNA was isolated from PTBP1 knockout or control MKN28 cells using Trizol reagent, and PolyA RNA was subsequently puri ed from total RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module. RNA-seq was performed to detect the mRNA expression pro les at GENTED (Shanghai, China) using HiSeq3000 (Illumina, USA). The differential genes were selected with fold change > 2 and a P-value < 0.05. To reveal PTBP1-bound mRNAs, RIP experiments were conducted using a PTBP1 antibody (Cell Signaling Technology) or IgG. Total RNA was isolated with Trizol (Invitrogen), and ribosomal RNA was removed from total RNA. RNAseq was performed at CLOUDSEQ (Shanghai, China) using HiSeq3000 (Illumina, USA). Data are available via GEO under accession numbers GSE157582 and GSE157941.
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