Nexera lc 40

The molecular weight and dispersity of the polymer were measured using Gel Permeation Chromatography (GPC, Nexera LC-40, Shimadzu, Kyoto, Japan). The morphologies of the PLCL nanofiber mat were characterized using a scanning electron microscope (SEM, JSM 6390 LV, Jeol, Tokyo, Japan) with 10 kV accelerating beam energy. The fiber size distribution of the electrospun PLCL nanofibers was determined by measuring 100 fibers in top-view SEM images. The thickness of the electrospun PLCL nanofiber mat was also measured by analyzing cross-sectional SEM images. The SEM specimens were prepared by cutting the nanofiber mat with sharp scissors at room temperature (RT). Note that, prior to the SEM analysis, the specimens were sputtered with gold (Mini Sputter Coater, SC 7620, Polaron, London, UK). The contact angle of the specimens was measured using a goniometer (OCA 15, DataPhysics Instruments, Filderstadt, Germany). The porosity of the specimens was also determined by comparing the total mass of the nanofiber mat with a neat polymer specimen of the same size.

The wastewater extracts were dissolved in water (1 mg/mL) and injected into a high-performance liquid chromatograph (Shimadzu Nexera LC-40, Shimadzu, Kyoto, Japan) with a UV-Vis detector. Separation was performed at 35 °C using a Phenomenex C18 reversed-phase column (250 mm × 4.6 mm, 5 μm; Torrance, CA, USA) with a binary gradient mixture of solvent A (ultrapure water/85% o-phosphoric acid = 99.8/0.2, v/v) and solvent B (methanol/acetonitrile = 1/1, v/v) at a flow rate of 1 mL/min: 0–16 min, 4–15% B; 16–50 min, 15–35% B; 50–62 min, 35–4% B; 65 min, 4% B. Phenolic compounds were identified by comparing retention times and absorption maxima at 220 and 320 nm with data obtained for corresponding standards and by sample spiking. Concentrations were expressed as mg of compound per gram of dry extract (mg/g).

HPLC analysis of carotenoids was performed on a Nexera LC-40 (Shimadzu, Kyoto, Japan) UHPLC system equipped with a diode-array detector SPD-M40, FCV-0607H high-pressure flow-line selection valve, CTO-40C column oven, SIL-40C XR autosampler, and LC-30D XR pump. The isocratic separation was carried out on a reversed-phase Kinetex (Phenomenex Inc, Torrance, USA) core–shell column, 150 mm × 3 mm i.d., particle size 2.6 µm (Phenomenex Inc, Torrance, USA), equipped with a Security Guard C18 3 × 4 mm i.d. (Phenomenex Inc, Torrance, USA). The autosampler was set to 4°C, and the column was thermostated at 30°C. The mobile phase consisted of methanol and methyl tert-butyl ether (95:5, v:v). Injection volume was 5 µl, the flow rate 0.8 mL/min, and runtime 15 min. The peak spectra were scanned from 300 to 800 nm.
The isolation of carotenoids from B and T lymphocytes was carried out as follows. The cells were pelleted 1,000 × g, 4°C, 2 min. The cell pellet was homogenized and carotenoids extracted using 2 mL of ice-cold acetone. The extract was filtered using a 0.22 µm PTFE syringe filter. The acetone extract was dried under a steady stream of argon. Dried samples were redissolved in 96% ethanol and immediately injected into the HPLC system.

The previous technique was used to evaluate the activity of FUT8 enzymes [42 (link)]. Donor (500 μM GDP-L-Fucose), assay buffer (200 mM MES), and the substrate (50 μM gngn-asn-4-(2-pyridylamine) butylamine (paba)) received five micrograms of cell lysates as an additional supply of enzymes. After incubation at 37°C about for 6 h, the mixture was heated at 100°C to halt the reaction for 5 min. The reactive solutions were centrifuged at 12,000 g for 10 min and then submitted to high performance liquid chromatography (Nexera LC-40, Shimadzu, Kyoto, Japan).