Cell motility and invasion were measured by transwell and Matrigel chamber plates, respectively (24-well format; 8-μm pore size; Corning Costar). Briefly, 1 × 105 cells were loaded per transwell cultured with serum-free media in the upside of the membrane. The cells were fixed in methanol for 10 min after migrating to the underside of the membrane and stained with crystal violet. Then, 30% glacial acetic acid was used to elute crystal violet to measure the cells on the lower face of the filter. Three independent experiments were performed in triplicate.
Matrigel chamber plates
Manufactured by Corning
Matrigel chamber plates are a laboratory product designed for cell culture applications. They provide a 3D extracellular matrix environment to support the growth and differentiation of various cell types. The plates are pre-coated with Matrigel, a complex mixture of basement membrane proteins, to facilitate the formation of physiologically relevant cell structures and interactions.
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Lab products found in correlation
2 protocols using matrigel chamber plates
1
Assessing Cell Motility and Invasion
2
Cell Proliferation, Migration, and Invasion Assays
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Cell proliferation was measured by CCK8 reagent (Boster, catalog no.AR1160) according to the manufacturer's instructions. Briefly, 2,000 cells were seeded in 96-well plates and incubated with 100 μL culture medium. An aliquot of 10 μL CCK8 was added and incubated for 2 hours. The absorbance at 450 nm was measured to calculate the numbers of viable cells. Migration and invasion assays were performed with transwell and Matrigel chamber plates (24-well format; 8-μm pore size; Corning Costar, catalog no. 3422) as described previously (32 ). ImageJ software was used to measure the cell area in the bottom chamber to quantify the cells that migrated across the filter. Each measurement was performed in triplicate, and the experiments were repeated three times.
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