Prom1

Manufactured by Thermo Fisher Scientific

Sourced in Ireland

The Prom1 is a versatile laboratory equipment designed for a range of applications. It functions as a high-performance microplate reader capable of measuring various types of assays. The Prom1 provides accurate and reliable data, making it a valuable tool for researchers and scientists.

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Lab products found in correlation

Oct compound, by Avantor (1 mentions) β catenin, by Cell Signaling Technology (1 mentions) Keratin 14, by Fortrea (1 mentions) Paraformaldehyde (pfa), by Polysciences (1 mentions) Tissue tek optimum cutting temperature, by Sakura Finetek (1 mentions) Anti rat cy3 cy5, by Jackson ImmunoResearch (1 mentions) Anti rabbit cy3 cy5, by Jackson ImmunoResearch (1 mentions) Dm5500b if microscope, by Leica (1 mentions) Cm1850, by Leica (1 mentions) Cell recovery solution, by Corning (1 mentions) Click it edu kit, by Thermo Fisher Scientific (1 mentions) Sox17, by R&D Systems (1 mentions) Hnf4α, by Santa Cruz Biotechnology (1 mentions) Krt19, by Agilent Technologies (1 mentions)

Spelling variants of this product

Anti-mouse Prom1/CD133 1∶100 (2 citations) PROM1-Biotin (2 citations)

3 protocols using prom1

Cryopreservation and Immunostaining of Skin

Cited 2 times

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For frozen sections the tissue was cryoproserved in Optimal Cutting Temperature (OCT) compound (VWR Chemicals, Lutterworth, UK #361603E) and sectioned at 5 μm thickness. Horizontal whole mounts were prepared as described previously (Driskell et al., 2012 (link), 2013 (link)). The following antibodies were used: Prom1 (1:50) (eBioscience, Hatfield, Ireland, UK, #14-1331-82), Keratin 14 (1:1000) (Covance, Cambridge, UK, #PRB-155P), Corin (1:100) (R&D systems, Minneapolis, MN, #AF2209), β-catenin (1:500) (Cell Signalling, Buckingham, UK, #8814S) and Ki67 (1:200) (Dako, Ely, UK, #M7249 clone TEC-3). All microscopy was performed on a Leica SP5 or Nikon A1 confocal microscope and images were analyzed using Image J (NIH).

Kaushal G.S., Rognoni E., Lichtenberger B.M., Driskell R.R., Kretzschmar K., Hoste E, & Watt F.M. (2015). Fate of Prominin-1 Expressing Dermal Papilla Cells during Homeostasis, Wound Healing and Wnt Activation. The Journal of Investigative Dermatology, 135(12), 2926-2934.

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Immunofluorescence and Immunohistochemistry of Liver

Cited 3 times

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Livers were fixed in 4% paraformaldehyde (Polysciences, Inc., Warrington, PA) and embedded in paraffin or Tissue-Tek® Optimum Cutting Temperature (OCT) Sakura Finetek, Torrance, CA) for sectioning. Immunofluorescence (IF) staining signals for PROM1 (1:50 dilution, eBiosciences, San Diego, CA), CYTOKERATIN-19 (1:100 dilution, gift from JR Friedman, University of Pennsylvania, Philadelphia. PA), and LacZ (1:400, Bioss, Woburn, MA) were detected by secondary antibodies conjugated either with anti-mouse cyanine (Cy) 3, Cy5, anti-rat Cy3/Cy5, or anti-rabbit Cy3/Cy5 (1:200 dilution, Jackson ImmunoResearch Labs, West Grove, PA) (9 (link), 10 (link), 20 (link)). Images were then acquired by a Leica DM5500B IF microscope using Leica Suite Advanced Fluorescence (LAS AF) 6000 software (Leica Microsystems, Wetzlar, Germany). Immunohistochemistry was performed using 2.1 μg/mL anti-INTEGRIN-β6 (gift from Shelia Violette, Biogen, Inc). Bright-field images were captured using a Leica DM1000 (DFC290) transmitted light microscope (Leica Microsystems AG, Heerbrugg, Switzerland) with Leica Application Suite (version 2.7.1R1).

Zagory J.A., Fenlon M., Dietz W., Zhao M., Nguyen M.V., Trinh P., Adoumie M., Park A., Xu J., Mahdi E., Glazier A., Malkoff N., Mavila N, & Wang K.S. (2019). PROMININ-1 promotes biliary fibrosis associated with biliary atresia. Hepatology (Baltimore, Md.), 69(6), 2586-2597.

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Liver Organoid Characterization Protocol

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Liver tissues were embedded in OCT (Tissue‐Tek), and slices of 10 μm thickness were cut using a cryostat (Leica CM 1850). Organoids were removed from Matrigel^® using Cell Recovery Solution (Corning) and then embedded in OCT and sliced, as described above. Tissue and organoid sections were fixed for 1 h in 4% paraformaldehyde at room temperature and stained using standard immunofluorescence techniques and commercially available antibodies (Yap1, Cell Signaling; Cldn3, GeneTex; Epcam, AbCAM; Prom1, E‐Bioscience; integrin A6, Millipore; Krt19, Dako; Hnf4α, Santa Cruz; Sox17, R&D; A6, gift from Valentina Factor, NIH). Nuclei were counterstained with DAPI, and images were acquired using a Zeiss 700 confocal microscope. Cell proliferation was assessed with the EdU Click‐it Kit (Life Technologies), and images were acquired with the organoids still embedded in Matrigel^® using the confocal microscope. To assess glycogen storage, organoids were examined by the periodic acid–Schiff staining method (PAS, Sigma‐Aldrich).

Lugli N., Kamileri I., Keogh A., Malinka T., Sarris M.E., Talianidis I., Schaad O., Candinas D., Stroka D, & Halazonetis T.D. (2016). R‐spondin 1 and noggin facilitate expansion of resident stem cells from non‐damaged gallbladders. EMBO Reports, 17(5), 769-779.

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