1290 series lc system

Manufactured by Agilent Technologies

Sourced in United States

The 1290 Series LC system is a high-performance liquid chromatography (HPLC) platform designed for analytical and preparative applications. It features a modular design, allowing users to configure the system based on their specific requirements. The system includes components such as a pump, autosampler, column compartment, and a detector, all of which are engineered to provide reliable and efficient separation and detection of samples.

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1290 series (38 citations) 1290 LC system (22 citations) Agilent 1290 series LC system (3 citations) Agilent 1290 Infinity series LC system (2 citations) Agilent 1290 series Rapid Resolution LC system (2 citations) 1290 Infinity II Series LC system (2 citations)

7 protocols using 1290 series lc system

Quantification of TBBPA and Methylation Products

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TBBPA was quantified by an Agilent 1290 Series LC system coupled with an Agilent 6460 triple quadrupole MS/MS system. Methylation products of TBBPA were detected by an Agilent 6890 gas chromatograph coupled with a model 5975C MS detector (Agilent Technologies, Palo Alto, CA). CH₃I was detected by an Agilent 7890A gas chromatograph coupled with a model 7000A triple quadrupole MS/MS (Agilent Technologies). The details of the instrument parameters were based on our previous study^{15 (link)} and are listed in Tables S1–S3.

Hou X., Kong W., Wang X., Liu Y., Chen W., Liu J., Schnoor J.L, & Jiang G. (2019). Abiotic Methylation of Tetrabromobisphenol A (TBBPA) with the Occurrence of Methyl Iodide in Aqueous Environments. Environmental science & technology letters, 6(9), 558-564.

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Trace Organic Contaminants Analysis

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Considering that more than 65,000,000 chemicals are currently available
commercially [18 ], this
present selected limited number of highly-representative indicator TOrCs (Table 1) that were previously reported in
wastewater and for which a robust analytical method has available [19 (link)]. In this paper, the analytical
method was further improved by using online solid-phase extraction
[20 ]. Briefly,
aliquots of the samples collected were spiked with stable isotopes of the
selected TOrC indicators, then 1.7 mL of sample was loaded onto a PLRP-S 15
μm cartridge using an Agilent 1290 FlexCube. Compounds retained on the
cartridge were desorbed directly onto an Agilent Poroshell 120 EC-C18 analytical
column (2.1 x 50 mm; 2.7 μm) then separated by liquid chromatography
(LC) using an Agilent 1290 series LC system with a gradient of water and
acetonitrile, both acidified with 0.1% acetic acid. Once eluted from the
column, compounds were detected by tandem mass spectrometry using an Agilent
6460 triple-quadrupole mass spectrometer using the electrospray source with fast
polarity switching (simultaneous analysis of positive and negative ions). This
method allowed detection limit in the range 0.1–15 ng/L depending on
compound properties.

Merel S., Anumol T., Park M, & Snyder S.A. (2014). Application of surrogates, indicators, and high-resolution mass spectrometry to evaluate the efficacy of UV processes for attenuation of emerging contaminants in water. Journal of hazardous materials, 282, 75-85.

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UHPLC-FLD Analysis of PSP Toxins

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The PSP toxins were determined using an Agilent Technologies (Wilmington, DE, USA) UHPLC–FLD system. The instrument consisted of an Agilent 1290 Series LC system with a high-pressure binary pump, a multi-sampler set at 4 °C, and an oven for the LC column set at 35 °C. The fluorescence detector was an Agilent 1260 Infinity II using excitation and emission wavelengths of 340 nm and 395 nm, respectively. For the chromatographic separation, an Atlantis T3 column (75 mm × 2.1 mm, 3 µm) was used, connected to an Atlantis T3 Vanguard cartridge (5 mm × 2.1 mm, 3 µm) (Waters, Milford, MA, USA). The mobile phase consisted of 0.1 M ammonium formate (A) and 0.1 M ammonium formate with 10% acetonitrile (B), both adjusted to pH 6 ± 0.1 with 0.1 M acetic acid, filtered by a Nylon filter membrane, and sonicated for 15 min. A gradient program with a flow of 0.7 mL min⁻¹ was run, starting with a 0.6 min isocratic at 5% B, followed by a linear increment to 45% B at 1.8 min. After an isocratic hold time of 2.2 min, in order to equilibrate the column, isocratic conditions at 0% B were held for 1.1 min, making the total run time 3.3 min. The injection volume was 15 µL. The version 2.4 of the OpenLab software (Agilent Technologies Spain, Madrid, Spain) was used to control the LC–FLD system and to acquire and process the data.
The LOQs of the method are given in Table S2 (Supplementary Materials).

Rodríguez-Cabo T., Moroño Á., Arévalo F., Correa J., Lamas J.P., Rossignoli A.E, & Blanco J. (2021). Paralytic Shellfish Poisoning (PSP) in Mussels from the Eastern Cantabrian Sea: Toxicity, Toxin Profile, and Co-Occurrence with Cyclic Imines. Toxins, 13(11), 761.

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Quantitative Analysis of Ginsenosides

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All analyses were performed on an Agilent 1290 Series LC system equipped with a binary pump, degasser, autosampler, column compartment, and variable wavelength absorbance detector set at 200 nm, with an Agilent 6410B electrospray ionization (ESI) triple quadrupole mass selective detector. All separations were carried out on an octadecyl ACE 3 C₁₈ column (Advanced Chromatography Technologies, Aberdeen, Scotland) with the following parameters: 150 mm length, 4.6 mm inner diameter, and 3 μm average particle size. The separation conditions were as follows: 0.7 mL/min flow rate, 25 °C column temperature, and a two solvent mobile phase system. Mobile phase A consisted of ACN with 0.1% formic acid and mobile phase B consisted of water with 0.1% formic acid. Gradient elution conditions began after 10 min, from 24% A to 58% A over 28 min, followed by a second gradient from 58% A to 100% A over 7 min. The separation concluded with a 15 min wash step at 100% A and a 10 min re-equilibration step at the starting conditions. The sample injection volume was 2 μL. Analyte detection was accomplished using multiple reaction monitoring (MRM) in negative and positive ion mode for ginsenosides and IS, respectively. Retention times and optimized MRM precursor-product ion transitions used for detection are listed in Table 1.

Hayes H.V., Wilson W.B, & Rimmer C.A. (2022). Method development for the determination of seven ginsenosides in three Panax ginseng reference materials via liquid chromatography with tandem mass spectrometry. Analytical and bioanalytical chemistry, 414(29-30), 8215-8222.

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Rapid LC-MS/MS Metabolite Quantification

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An Agilent 1290 series LC system consisting of a binary pump and an autosampler was used for LC separation with an Agilent Zorbax Eclipse Plus C18 column (50×2.1 mm i.d., 1.8 μm; USA) at 35°C. The flow rate of the mobile phase was 0.2 mL min⁻¹, and the injection volume was 2 μL. The initial mobile phase was 30% A (methanol) and 70% B (water containing 5 mmol L⁻¹ ammonium acetate and 0.25% FA), followed by a linear gradient to 90% A in 5 min, and kept isocratic in 2 min, and then back to 30% A in 0.5 min.

Pei J., Zhang R., Hsu C, & Wang Y. (2018). Mass Spectrometry-Inspired Degradation of Disinfection By-Product, 2,6-Dichloro-1,4-benzoquinone, in Drinking Water by Heating. Mass Spectrometry, 7(1), A0068.

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Quantitative Mass Spectrometry Proteomics

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Analysis of the digested samples is performed on an Agilent 6460 triple quadrupole mass spectrometer in positive ion mode equipped with an Agilent 1290 Series LC system utilizing an Agilent Zorbax 300 SB-C18 column (2.1 mm × 150 mm, 3.5 μm). The column temperature is maintained at 45°C, and the peptides were loaded onto the column at a flow rate of 200 μL/min in 97% (volume fraction) mobile phase A (water with 1 mL/L formic acid) and 3% (volume fraction) mobile phase B (ACN with 1 mL/L formic acid). General mass spectrometric conditions: gas temperature of 300°C; gas flow of 7 L/min; nebulizer of 20 psi (1.4 × 10⁵ Pa); sheath gas temperature of 300°C; sheath gas flow of 6 L/min; capillary voltage of 4000 V; and a nozzle voltage of 1500 V. Analysis of the process samples is conducted in a randomized sequence with replicate measurements to reduce influence of systematic bias on the output measurements.

Beasley-Green A, & Heckert N.A. (2023). Estimation of measurement uncertainty for the quantification of protein by ID-LC–MS/MS. Analytical and Bioanalytical Chemistry, 415(16), 3265-3274.

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Quantitative Analysis of Micafungin in Plasma

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All experiments were performed on an Agilent 6460A (CA, USA) triple quadrupole LC-MS/MS system, with a combined Agilent 1290 series LC system. The Agilent 1290 Infinity system consists of a binary LC-pump and thermostatic column compartment. The Agilent 6460A mass selective detector operated in heated electrospray negative ionization mode and performed single ion monitoring (SIM) with unit mass resolution. A second automated pump, Merck Hitachi L-7110, was used to rinse the source, when the LC flow was diverted to the waste. A 100 × 2.1 mm 3 μm Waters Atlantis T3 analytical column from Waters Corporation (MA, USA) was used for chromatography. Plasma concentrations of micafungin were calculated using the peak area ratios of the micafungin and [ 13 C 6 ]-micafungin. For quantitative analysis of the analytical results the Agilent Masshunter software was used.

Boonstra J.M., Jongedijk E.M., Koster R.A., Touw D.J, & Alffenaar J.C. (2018). Simple and robust LC-MS/MS analysis method for therapeutic drug monitoring of micafungin. Bioanalysis, 10(11).

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