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Pi31430

Manufactured by Thermo Fisher Scientific
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The PI31430 is a laboratory instrument designed for temperature measurement and control. It features a digital display and provides precise temperature readings. The device is suitable for use in a variety of laboratory settings, but a detailed description of its intended use or capabilities is not available.

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Lab products found in correlation

Anti calreticulin, by Cell Signaling Technology (2 mentions) Anti pkm1, by Cell Signaling Technology (2 mentions) Anti p70 s6 kinase, by Cell Signaling Technology (2 mentions) Anti pkm, by Abcam (2 mentions) Anti pkm2, by Cell Signaling Technology (2 mentions) Anti human specific ldha, by Cell Signaling Technology (2 mentions) Anti ldhb, by Abcam (2 mentions) Anti human and rabbit ldha, by Abcam (2 mentions) Anti golgin, by Cell Signaling Technology (2 mentions) Anti cox 4, by Proteintech (2 mentions) Anti gpt2, by Santa Cruz Biotechnology (2 mentions) Anti phgdh, by Merck Group (2 mentions) Anti pc, by Santa Cruz Biotechnology (2 mentions) Pi 31460, by Thermo Fisher Scientific (2 mentions) Anti β actin, by Merck Group (2 mentions)

2 protocols using pi31430

1

Western Blot Analysis of Metabolic Enzymes

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell lysates were prepared and subjected to WB analysis as performed previously51 (link),52 (link). The following commercial antibodies were used: anti-β-actin (Sigma A1978), anti-PKM2 (Cell Signaling Technology 3198S), anti-PKM1 (Cell Signaling Technology 7067), anti-PKM (Abcam AB118499), anti-human specific LDHA (Cell Signaling Technology 2012S), anti-LDHB (Abcam AB75167), anti-human and rabbit LDHA (Abcam AB135396), anti-p70 S6 kinase (Cell Signaling Technology 2708T), anti-calreticulin (Cell Signaling Technology 12238T), anti-golgin (Cell Signaling Technology 13192), anti-COX IV (Proteintech 11242-1-AP), anti-GPT2 (Santa Cruz sc-398383), anti-PHGDH (Sigma HPA021241), and anti-PC (Santa Cruz sc-271493), horseradish peroxidase-conjugated secondary antibodies anti-mouse (Fisher PI31430) and anti-rabbit (Fisher PI31460). All the primary antibodies were used at a 1:500 dilution in 5% non-fat milk in TBST. Secondary antibodies were used at a 1:3000 dilution in 5% non-fat milk in TBST.
Wiese E.K., Hitosugi S., Loa S.T., Sreedhar A., Andres-Beck L.G., Kurmi K., Pang Y.P., Karnitz L.M., Gonsalves W.I, & Hitosugi T. (2021). Enzymatic Activation of Pyruvate Kinase Increases Cytosolic Oxaloacetate to Inhibit the Warburg Effect. Nature metabolism, 3(7), 954-968.
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2

Western Blot Analysis of Metabolic Enzymes

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell lysates were prepared and subjected to WB analysis as performed previously51 (link),52 (link). The following commercial antibodies were used: anti-β-actin (Sigma A1978), anti-PKM2 (Cell Signaling Technology 3198S), anti-PKM1 (Cell Signaling Technology 7067), anti-PKM (Abcam AB118499), anti-human specific LDHA (Cell Signaling Technology 2012S), anti-LDHB (Abcam AB75167), anti-human and rabbit LDHA (Abcam AB135396), anti-p70 S6 kinase (Cell Signaling Technology 2708T), anti-calreticulin (Cell Signaling Technology 12238T), anti-golgin (Cell Signaling Technology 13192), anti-COX IV (Proteintech 11242-1-AP), anti-GPT2 (Santa Cruz sc-398383), anti-PHGDH (Sigma HPA021241), and anti-PC (Santa Cruz sc-271493), horseradish peroxidase-conjugated secondary antibodies anti-mouse (Fisher PI31430) and anti-rabbit (Fisher PI31460). All the primary antibodies were used at a 1:500 dilution in 5% non-fat milk in TBST. Secondary antibodies were used at a 1:3000 dilution in 5% non-fat milk in TBST.
Wiese E.K., Hitosugi S., Loa S.T., Sreedhar A., Andres-Beck L.G., Kurmi K., Pang Y.P., Karnitz L.M., Gonsalves W.I, & Hitosugi T. (2021). Enzymatic Activation of Pyruvate Kinase Increases Cytosolic Oxaloacetate to Inhibit the Warburg Effect. Nature metabolism, 3(7), 954-968.
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