Log In Sign up
The largest database of trusted experimental protocols

C10337

Manufactured by Thermo Fisher Scientific
Visit Supplier
Sourced in United States

The C10337 is a laboratory instrument from Thermo Fisher Scientific. It is designed for performing various analytical and measurement tasks in a research or testing environment. The core function of the C10337 is to provide accurate and reliable data acquisition and processing capabilities for scientific applications. Further details on the intended use or specific features of this product are not available.

Automatically generated - may contain errors

Lab products found in correlation

Lsm 710 confocal microscope, by Zeiss (6 mentions) Hoechst 33342, by Thermo Fisher Scientific (4 mentions) 5 ethynyl 2 deoxyuridine (edu), by Thermo Fisher Scientific (3 mentions) Sting, by GenScript (2 mentions) Phospho s222 mvb12b, by GenScript (2 mentions) T511307, by Merck Group (2 mentions) Cryostat, by Leica camera (2 mentions) C10638, by Thermo Fisher Scientific (2 mentions) Axio imager m2, by Zeiss (2 mentions) Tunel assay, by Merck Group (2 mentions) S7110, by Merck Group (2 mentions) Lsm 710, by Zeiss (2 mentions) Lsm 880 confocal microscope, by Zeiss (2 mentions) Bond rx, by Leica camera (2 mentions) E2f3a, by Merck Group (2 mentions) Ph3s10, by Merck Group (2 mentions) Myc tag, by Cell Signaling Technology (2 mentions) Ventana discovery ultra, by Roche (2 mentions) N3633, by Merck Group (2 mentions) B5002, by Thermo Fisher Scientific (2 mentions)

52 protocols using c10337

1

Fgf8.L Expression and Cell Proliferation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ex vivo limbs were cultured with 10 μM EdU (ThermoFisher Scientific, C10337) for 3 days in dark foiled cover. Afterwards, samples were fixed and Fgf8.L mRNA was stained using the HCR protocol, followed by cryosectioning, as described above. Sections were subjected to the Click-It reaction, as described in the manufacturer's protocol (ThermoFisher Scientific, C10337). Hoechst was added at the end of the protocol. Samples were visualized by confocal microscopy as described above. Fgf8.L-positive cells, and both EdU- and Fgf8.L-positive cells on the amputation plane were manually counted, and the percentage of EdU-positive Fgf8.L-positive cells were calculated for each sample.
Aztekin C., Hiscock T.W., Gurdon J., Jullien J., Marioni J, & Simons B.D. (2021). Secreted inhibitors drive the loss of regeneration competence in Xenopus limbs. Development (Cambridge, England), 148(11), dev199158.
+ Open protocol
+ Expand
2

Proliferation Assay for Hepatocyte Organoids

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human hepatocyte organoids were transferred to 96-well µ-clear bottom plates (Greiner Bio-One, 655090) for EdU proliferation assay (Thermo Fisher Scientific, C10214 and C10337). Hepatocyte organoids were fixed in 4% PFA (Thermo Fisher Scientific, J19943) o/n at 4 °C. On the next day, organoids were washed thrice with PBS+ containing 0.1% Triton X-100 (wash buffer, AppliChem) and blocked for 30 min at RT in PBS+ containing 0.1% Triton X-100, 10% NDS and 1% BSA (blocking solution). The EdU proliferation assay was performed according to the manufacturer’s instructions (Thermo Fisher Scientific, C10214 and C10337). For (additional) whole mount staining, organoids were washed thrice with wash buffer and incubated with rabbit anti-HNF4α (Cell Signaling, C11F12, 3113, 1/250) diluted in blocking solution for 2 h at RT or o/n at 4 °C. Both before and after the addition of the secondary antibody, the organoids were washed thrice in wash buffer. The antibodies were as follows: donkey anti-rabbit Alexa Fluor 555 (Invitrogen, A-31572, 1/500) and DAPI (Sigma-Aldrich, D9542, 1/1000). The incubation time was 2 h at RT. All steps were performed under agitation. (Z-stack) images were acquired using LSM (LSM 710, Zeiss), and analyses were done with Fiji. Organoid area was measured with a free hand selection tool, and EdU+ cells were counted manually in a blinded manner.
Große-Segerath L., Follert P., Behnke K., Ettich J., Buschmann T., Kirschner P., Hartwig S., Lehr S., Korf-Klingebiel M., Eberhard D., Lehwald-Tywuschik N., Al-Hasani H., Knoefel W.T., Heinrich S., Levkau B., Wollert K.C., Scheller J, & Lammert E. (2024). Identification of myeloid-derived growth factor as a mechanically-induced, growth-promoting angiocrine signal for human hepatocytes. Nature Communications, 15, 1076.
+ Open protocol
+ Expand
3

Multicolor Visualization of Replicating Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells grown until A 600 of ~0.1 were labelled with 10 μM EdU (5-Ethynyl-2´-deoxyuridine, Thermofisher, C10337) for 15 min after which cells were washed, introduced to fresh media containing 60 μg/ml thymidine and allowed to grow for 3 h. Following this, cells were fixed with 4% PFA (v/v) for 30 min and permeabilized with 0.5% Triton X-100 (v/v) for 30 min. EdU click-chemistry reaction was conducted following the instructions (Thermofisher, #C10337) using Alexa 488 azide in a final volume of 50 μl for 30 min at room temperature, followed by washing. Cells were then labelled with TMR HaloTag ligand as in (Banaz et al., 2019) . Briefly, cells were incubated with 2 μM TMR ligand for 30 min and washed several times. Finally, nucleoids were labelled with 1 μg/ml DAPI for 15 min and washed, after which the cells were ready for imaging.
Mäkelä J., Uphoff S., & Sherratt D.J. (2020). Non-random segregation of sister chromosomes by Escherichia coli MukBEF axial cores.
+ Open protocol
+ Expand
4

Quantifying Fibroblast Proliferation via EdU

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fibroblasts were pulsed for 1 h with 10 μM EdU (Invitrogen C10337) at 37 °C to label cells in S-phase and then fixed with 4% PFA for 15 min at room temperature. Cells were then treated per the manufacturer’s recommendations to visualize EdU through click-chemistry (Invitrogen C10337). Cells were stained with Hoechst to label nuclei (1:10,000; Thermo Fisher Scientific 62249). Samples were then analyzed for the percentage of cells that were EdU+ .
Morrow C.S., Arndt Z.P., Klosa P.C., Peng B., Zewdie E.Y., Benayoun B.A, & Moore D.L. (2022). Adult fibroblasts use aggresomes only in distinct cell-states. Scientific Reports, 12, 15001.
+ Open protocol
+ Expand
5

Induction of Cre Expression and DNA Synthesis Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
To induce Ah-cre expression, 80mg kg−1 body weight of β-naphthoflavone (Sigma-Aldrich; N3633) dissolved in corn oil (Sigma-Aldrich; C8267) was administrated into 2-month old mice with 5 injections within 30 hours. For DNA synthesis detection, 100mg kg−1 body weight of BrdU (Sigma-Aldrich; B5002) or 5mg kg−1 body weight of EdU (Life Technologies; C10337) dissolved in sterile phosphate-buffered saline (PBS) was intraperitoneally injected 2 hours or 1 hour before the mice were sacrificed, respectively. EdU staining was performed following manufacturer’s protocol (Life Technologies; C10337).
Liu H., Tang X., Srivastava A., Pécot T., Daniel P., Hemmelgarn B., Reyes S., Fackler N., Bajwa A., Kladney R., Koivisto C., Chen Z., Wang Q., Huang K., Machiraju R., Sáenz-Robles M.T., Cantalupo P., Pipas J.M, & Leone G. (2015). Redeployment of Myc and E2f1-3 drives Rb deficient cell cycles. Nature cell biology, 17(8), 1036-1048.
+ Open protocol
+ Expand
6

Induction of Cre Expression and DNA Synthesis Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
To induce Ah-cre expression, 80mg kg−1 body weight of β-naphthoflavone (Sigma-Aldrich; N3633) dissolved in corn oil (Sigma-Aldrich; C8267) was administrated into 2-month old mice with 5 injections within 30 hours. For DNA synthesis detection, 100mg kg−1 body weight of BrdU (Sigma-Aldrich; B5002) or 5mg kg−1 body weight of EdU (Life Technologies; C10337) dissolved in sterile phosphate-buffered saline (PBS) was intraperitoneally injected 2 hours or 1 hour before the mice were sacrificed, respectively. EdU staining was performed following manufacturer’s protocol (Life Technologies; C10337).
Liu H., Tang X., Srivastava A., Pécot T., Daniel P., Hemmelgarn B., Reyes S., Fackler N., Bajwa A., Kladney R., Koivisto C., Chen Z., Wang Q., Huang K., Machiraju R., Sáenz-Robles M.T., Cantalupo P., Pipas J.M, & Leone G. (2015). Redeployment of Myc and E2f1-3 drives Rb deficient cell cycles. Nature cell biology, 17(8), 1036-1048.
+ Open protocol
+ Expand
7

5-ethnyl-2′-deoxyuridine Cell Labeling Protocol

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
One hour prior to euthanasia, mice received a single intraperitoneal injection of 5-ethnyl-2′-deoxyuridine (ThermoFisher A10044) at a dose equimolar to that previously described8 (link) for BrdU (40 μg/g body weight). Tissue was then fixed in formaldehyde and processed in graded sucrose for cryosectioning, as described above. Tissue sections (50 µm thick) were permeabilized for 1 h at room temperature in PBS containing 3% BSA and 0.5% Tween-20, followed by copper catalyzed click labeling with an Alexa 488 azide conjugate (ThermoFisher #C10337) for 45′ at room temperature, several washes in PBS/BSA, and confocal imaging.
Venables T., Griffith A.V., DeAraujo A, & Petrie H.T. (2019). Dynamic changes in epithelial cell morphology control thymic organ size during atrophy and regeneration. Nature Communications, 10, 4402.
+ Open protocol
+ Expand
8

EdU Incorporation Assay for Proliferation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were seeded at a density of 10,000 cells/cm2 in ibidi 8-well plates. After 24 h a 2X working solution of EdU (C10337, ThermoFisher) in EGM was added to the cells for 6 h. 4% paraformaldehyde (PFA) was added to the cell medium for 15 min before washing with 3% BSA in PBS and then 0.5% Triton X- for 20 min. Cells were washed again with 3% BSA before the addition of a Click-iT reaction cocktail (Click-iT reaction buffer, CuSO4 (Component E), Alexa Fluor Azid and Click-iT buffer additive) for 30 min at RT. Cells were washed and incubated in Hoechst 33,342 (Component G) solution 1:2000 in PBS (5 μg/mL) for a further 30 min at RT before washing with PBS. Cells were imaged for Hoechst and EdU (488 nm) with a laser scanning confocal microscope (LSM800, Zeiss) and images quantified with FIJI/ImageJ.56 (link)
Oo J.A., Pálfi K., Warwick T., Wittig I., Prieto-Garcia C., Matkovic V., Tomašković I., Boos F., Izquierdo Ponce J., Teichmann T., Petriukov K., Haydar S., Maegdefessel L., Wu Z., Pham M.D., Krishnan J., Baker A.H., Günther S., Ulrich H.D., Dikic I., Leisegang M.S, & Brandes R.P. (2022). Long non-coding RNA PCAT19 safeguards DNA in quiescent endothelial cells by preventing uncontrolled phosphorylation of RPA2. Cell Reports, 41(7), 111670.
+ Open protocol
+ Expand
9

Imaging Nuclear Translocation and Colocalization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Experiments were carried out on cells seeded on cover slips. Cells were fixed with ice-cold methanol for 5 min at -20°C, washed with PBS and stained with DAPI, following which they were mounted onto glass slides using Prolong Gold. For nuclear translocation and co-localization assays, cells were fixed and permeabilized with 4% paraformaldehyde and 0.1% Triton-X. The cells were then blocked with 2.5% BSA and stained with the following antibodies: Rab7 (9367, Cell Signaling.), IRF3 (Cell Signaling, 4302), STING (AF6516), phospho S222 MVB12b (GenScript, Antibody production service) and corresponding secondary antibodies. Images were obtained on Zeiss LSM 710 confocal microscope using a 63× 1.4 oil-immersion objective and data were analyzed using ImageJ software. For click-it chemistry, EdC-labeled (T511307,Sigma) DNA of bacteria were detected in fixed cells according to manufacturer’s instructions (ThermoFisher, C10337) and AlexaFluor488 was detected under the microscope.
Nandakumar R., Tschismarov R., Meissner F., Prabakaran T., Krissanaprasit A., Farahani E., Zhang B.C., Assil S., Martin A., Bertrams W., Holm C.K., Ablasser A., Klause T., Thomsen M.K., Schmeck B., Howard K.A., Henry T., Gothelf K.V., Decker T, & Paludan S.R. (2019). Intracellular bacteria engage a STING-TBK1-MVB12b pathway to enable paracrine cGAS-STING signaling. Nature microbiology, 4(4), 701-713.
+ Open protocol
+ Expand
10

EdU Incorporation Detection in Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
EdU (Invitrogen) dissolved in water was injected intraperitoneally at 10 μg/g body weight at 4 h before harvest. EdU incorporation was detected by a click reaction according to manufacturer’s instructions (Thermo Fisher Scientific, C10337). Images were acquired with the Nikon C-1 confocal system.
Song D., He G., Shi Y., Ni J, & Long F. (2021). Functional interaction between Wnt and Bmp signaling in periosteal bone growth. Scientific Reports, 11, 10782.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!