P dab

Nine reagents were used in this experiment. Dragendorff, p-DAB, and Folin-Ciocalteu reagent were directly used from Merck. Ferric chloride 5% was made in water. Sodium nitroprusside 1% was made in water. Schiff reagent was made by dissolving 200 mg of base fuchsin in 120 mL of hot water and letting it cool. Then, we added 2 g of sodium sulfite anhydrous in 20 mL of water, added 2 mL of concentrated HCl, diluted with water up to 200 mL, and left it for at least 2 hours. the p-DAB reagent was made by dissolving 2 g of p-DAB into 50 mL ethanol 95% and 50 mL concentrated HCl; reagents must be made fresh. Amalic acid test was performed using HCl 10 M, potassium chlorate, and ammonium hydroxide 2 M as a reagent. Tollens reagent was made by dissolving silver nitrate in 100 mL of aquadest, and 5 mL of NaOH 5% was added. After that 7.5 mL of concentrated ammonium hydroxide was added. Diazotization test was carried out by dissolving the sample in HCl 2 M, adding 1 drop sodium nitrite 1%, and adding 1 drop of 2-naft-2-ol 4% in NaOH 2 M. These reagents were then tested against allopurinol solution (100 mg/ml) and NaOH 2 N as a blank reagent to observe the color changes.

All chemicals used were of analytical grade, and they were used without further purification. All solutions were made using distilled water. Allopurinol was obtained from Nanjing Pharma Chemical Plant. 2-Naphthol, ammonium hydroxide 25%, hydrochloric acid 37%, bismuth subnitrate, ethanol 95%, ferric chloride, potassium hydroxide, potassium iodide, sodium hydroxide, potassium chlorate crystals, sodium nitrite, sodium nitroprusside, sodium sulfite anhydrous, silver nitrate, Dragendorff reagent, p-DAB, and Folin-Ciocalteu reagent were purchased from Merck. Alkaline fuchsin was purchased from Sigma Aldrich, and sample packaged herbs were purchased from traditional drug store around Jatinangor, Sumedang, West Java. Whatman No. 1, No. 4, and No. 6 and Whatman 1 chromatography qualitative-grade filter paper were purchased from GE Healthcare. The absorbance measurement was recorded by UV-Visible spectrophotometer Analytik Jena SPECORD 200 using a 1.0 cm quartz cell.