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Sinapinic acid matrix solution

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Sinapinic acid matrix solution is a compound used in matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. It serves as a matrix to facilitate the ionization and desorption of analytes, enabling their detection and analysis by the mass spectrometer.

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Lab products found in correlation

Sinapinic acid, by Merck Group (3 mentions) Trifluoroacetic acid, by Merck Group (3 mentions) Flexanalysissoftware 3, by Bruker (2 mentions) Ultraflextreme maldi tof tof mass spectrometer, by Bruker (2 mentions) Proteins maldi mscalibration kit, by Merck Group (2 mentions) Acetonitrile, by Merck Group (1 mentions) Ultraflex 2 maldi tof tof mass spectrometer, by Bruker (1 mentions)

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Sinapinic acid (15 citations) Matrix solution (3 citations)

3 protocols using sinapinic acid matrix solution

1

MALDI-TOF Analysis of GABAB1b-GABAB2 Heterodimer

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Purified GABAB1b-GABAB2 complex (0.3 mg/ml, 1
μl) was mixed with 1 μl sinapinic acid matrix solution (Bruker
Daltonics, Billerica, USA) containing 10 mg of sinapinic acid in 1 ml of 2.5%
trifluoroacetic acid (MilliporeSigma, Burlington, USA) and 50% acetonitrile
(MilliporeSigma, Burlington, USA). The protein-matrix suspension (2 μl)
was added to the ground steel MALDI target plate and dried at room temperature.
Mass spectra were collected using a UltrafleXtreme MALDI-TOF/TOF mass
spectrometer (Bruker Daltonic, Billerica, USA) operated with FlexControl
software in linear positive mode, i.e. using a mass range of 30,000 to 120,000
daltons. The instrument was externally calibrated with Proteins MALDI-MS
calibration kit (MilliporeSigma, Burlington, USA). Each individual mass spectrum
was analyzed and adjusted for smoothness and baseline using FlexAnalysis
software 3.0 (Bruker Daltonics, Billerica, USA). The molecular mass of the
heterodimeric GABAB1b-GABAB2 complex was determined to be
192,647.967 daltons.
Park J., Fu Z., Frangaj A., Liu J., Mosyak L., Shen T., Slavkovich V.N., Ray K.M., Taura J., Cao B., Geng Y., Zuo H., Kou Y., Grassucci R., Chen S., Liu Z., Lin X., Williams J.P., Rice W.J., Eng E.T., Huang R.K., Soni R.K., Kloss B., Yu Z., Javitch J.A., Hendrickson W.A., Slesinger P.A., Quick M., Graziano J., Yu H., Fiehn O., Clarke O.B., Frank J, & Fan Q.R. (2020). Structure of human GABAB receptor in an inactive state. Nature, 584(7820), 304-309.
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2

MALDI-TOF Analysis of GABAB1b-GABAB2 Heterodimer

Check if the same lab product or an alternative is used in the 5 most similar protocols
Purified GABAB1b-GABAB2 complex (0.3 mg/ml, 1
μl) was mixed with 1 μl sinapinic acid matrix solution (Bruker
Daltonics, Billerica, USA) containing 10 mg of sinapinic acid in 1 ml of 2.5%
trifluoroacetic acid (MilliporeSigma, Burlington, USA) and 50% acetonitrile
(MilliporeSigma, Burlington, USA). The protein-matrix suspension (2 μl)
was added to the ground steel MALDI target plate and dried at room temperature.
Mass spectra were collected using a UltrafleXtreme MALDI-TOF/TOF mass
spectrometer (Bruker Daltonic, Billerica, USA) operated with FlexControl
software in linear positive mode, i.e. using a mass range of 30,000 to 120,000
daltons. The instrument was externally calibrated with Proteins MALDI-MS
calibration kit (MilliporeSigma, Burlington, USA). Each individual mass spectrum
was analyzed and adjusted for smoothness and baseline using FlexAnalysis
software 3.0 (Bruker Daltonics, Billerica, USA). The molecular mass of the
heterodimeric GABAB1b-GABAB2 complex was determined to be
192,647.967 daltons.
Park J., Fu Z., Frangaj A., Liu J., Mosyak L., Shen T., Slavkovich V.N., Ray K.M., Taura J., Cao B., Geng Y., Zuo H., Kou Y., Grassucci R., Chen S., Liu Z., Lin X., Williams J.P., Rice W.J., Eng E.T., Huang R.K., Soni R.K., Kloss B., Yu Z., Javitch J.A., Hendrickson W.A., Slesinger P.A., Quick M., Graziano J., Yu H., Fiehn O., Clarke O.B., Frank J, & Fan Q.R. (2020). Structure of human GABAB receptor in an inactive state. Nature, 584(7820), 304-309.
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3

Leptospira MALDI-TOF Mass Spectrometry

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Leptospira was cultured for a week to obtain 108 colony forming unit (CFU) per ml as previously described [14 (link)]. Three milliliters of culture were centrifuged at 13,000 g for 2 min at room temperature and cell pellet was washed with 70% ethanol and centrifuged at 13,000 g for 2 min. The pellet was mixed with 50 μl sinapinic acid matrix solution (Bruker Daltonics, Germany) containing 10 mg of sinapinic acid in 1 ml of 2.5% trifluoroacetic acid (Sigma-Aldrich, USA) and 50% acetonitrile (Sigma-Aldrich, USA). After being well mixed by pipetting and centrifuged at 13,000 g for 1 min, then 2 μl of cell-matrix suspension were added to the ground steel MALDI target plate and dried at room temperature. Each isolates were spotted 24 dots onto the MALDI target plate to test technical replication. Mass spectra were collected using a Ultraflex II MALDI-TOF/TOF mass spectrometer (Bruker Daltonic, USA) operated with FlexControl software in linear positive mode, i.e. using a mass range of 2,000 to 20,000 Daltons. The instrument was externally calibrated with E. coli strain DH5α ribosomal proteins as recommended protocol.
Sonthayanon P., Jaresitthikunchai J., Mangmee S., Thiangtrongjit T., Wuthiekanun V., Amornchai P., Newton P., Phetsouvanh R., Day N.P, & Roytrakul S. (2019). Whole cell matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for identification of Leptospira spp. in Thailand and Lao PDR. PLoS Neglected Tropical Diseases, 13(4), e0007232.
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