H1184

Human tumor cell lines were cultured in DMEM/10% FCS under a humidified 5% CO₂ atmosphere at 37 °C. The following cell lines were used: A-172, A549, A64-CLS, Hela, HL-60, LNCaP, MCF-7, MG-63, PC-3, SaOs, and SW-480 from Cell Lines Service (Eppelheim, Germany); B-CPAP, BHY, CAL-62, COLO320HSR, H1184, H146, HT29, LoVo, SKN-MC, SW-403, and THP-1 from DSMZ (Braunschweig, Germany); FTC-133, U251-MG, and U373-MG from Sigma-Aldrich (Taufkirchen, Germany); and SW948 and U87-MG from American Type Culture Collection (Manassas, VA, USA), as previously described [18 (link)].

SCLC cell lines GLC-2 [17] (link) and -36 [18] (link) were provided by Prof. Dr. L.F.M.H. de Leij (University of Groningen, The Netherlands) and SCLC-24H [19] (link) by Dr. H. Lahm (Thoraxklinik Heidelberg, Germany). SCLC cell line H146 was purchased from American Type Culture Collection (Rockville, USA) and SCLC cell line H1184 from DSMZ (Braunschweig, Germany). Cell lines were maintained in DMEM, 10% fetal calf serum (FCS) or in DMEM/Ham'sF12, 20% FCS (cell line H1184). For DNA profiling, 15 different short tandem repeat (STR) systems and the gender specific amelogenin locus were amplified from 1 to 5 ng genomic DNA in a multiplex-PCR using the PowerPlex® 16 System (Promega, Madison, WI, USA), dedicated to forensic DNA profiling, according to manufacturer's instructions. PCR products were quality controlled utilizing a micro fluidic ‘DNA 7500’ array on a 2100 Bioanalyzer (Agilent, Böblingen, Germany). If quality control was passed, allele detection and identification was performed by capillary electrophoresis performed on a 310 Genetic Analyzer (Applied Biosystems, Darmstadt, Germany). Data processing and analysis was done using the GeneMapper software (v3.2) (Applied Biosystems). The DNA profiles of the cell lines are shown in Table 1.