B6 cg rorctm3litt j

The following mice strains used were purchased from Jackson Laboratory: C57BL/6J (B6), Rag1^−/−, B6(Cg)-Rorc^tm3Litt/J. For all the experiment, 6–10 weeks old mice were used. Antibodies were purchased from either eBioscience, BD Biosciences or Fisher scientific for example-CD3, CD4, CD25, CD45RB, T-bet (eBio4B10), FoxP3 (FJK-16S), RORγt (AFKJS-9), IL-17A (TC11–18H10 or eBio17B7), IFNγ (XMG1.2).

C57BL/6Prdm1^{flox/floxCD4−Cre+} and C57BL/6Prdm1^{flox/floxFoxp3−Cre+} were described (Bankoti et al., 2017 (link); Salehi et al., 2012 (link)). C57BL/6 B6.129S7-Rag1^tm1Mom/J (RAG^−/−), B6.SJL-^PtprcaPep3b/BoyJ (CD45.1⁺), B6.129P2-Il10^tm1Cgn/J (IL10^−/−), C57BL/6-Il17a^tm1Bcgen/J (IL17^eGFP) and Foxp3^RFP (C57BL/6-Foxp3^tm1Flv/J) reporter mice and mice with “floxed” RORC alleles (B6(Cg)-Rorc^tm3Litt/J) were obtained from Jackson labs. Foxp3-IRES-GFP knock-in (Foxp3^GFP) (Bettelli et al., 2008 (link)) mice were obtained from Dr. V. Kuchroo (Harvard Medical School) and crossed to IL10^−/− mice to generate IL10KOFoxp3^GFP. Blimp1^YFP reporter mice were obtained from Dr. Eric Meffre (Yale University) and bred to Foxp3^RFP mice to generated Blimp1^YFPFoxp3^RFP dual reporter mice. Foxp3^RFP mice were also bred to ROR(γt)^GFP mice or to IL17Ae^GFP mice to generate Foxp3^RFPIL17^GFP or Foxp3^RFP/RORγt^GFP dual reporter mice or to C57BL/6Prdm1^flox/flox mice to generated Prdm1^flox/floxFoxp3^RFP mice. Foxp3^YFP-CRE mice were bred to C57BL/ 6Prdm1^flox/flox mice to generate mice with Treg-specific deletion of Blimp-1 and then bred to Rorc^flox/flox mice to generate mice with Treg-specific double deficiency of Blimp-1 and Rorγt. All mice were bred and maintained in the CSMC SPF animal barrier facility and handled in accordance with the institutional guidelines.