Nlrp3 sirna

Manufactured by GenePharma

Sourced in China

The NLRP3 siRNA is a laboratory reagent designed for gene silencing experiments. It targets the NLRP3 gene, which is involved in the innate immune response. The NLRP3 siRNA can be used to temporarily suppress the expression of the NLRP3 gene in cell culture models, allowing researchers to study its cellular functions.

Automatically generated - may contain errors

Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (3 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (3 mentions) Lipofectamine 3000 reagent, by Thermo Fisher Scientific (2 mentions) Nc sirna, by GenePharma (2 mentions) Nc sirna, by Thermo Fisher Scientific (2 mentions) Bovine serum albumin (bsa), by MedChemExpress (1 mentions) Brcc3 sirna, by GenePharma (1 mentions) Vdr sirna, by GenePharma (1 mentions) Opti mem 1 reduced serum medium, by Thermo Fisher Scientific (1 mentions) Mmp 1 sirna, by GenePharma (1 mentions) Hsf1 sirna, by GenePharma (1 mentions) Pink1 sirna, by GenePharma (1 mentions) Opti mem, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

SiRNAs (565 citations) NLRP3 (4 citations) Small interfering RNA (siRNA) against NLRP3 (3 citations)

11 protocols using nlrp3 sirna

Chrysophanol Modulates NLRP3 Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were uniformly seeded into 6-well plates at 5×10 5 cells/well with different concentrations of chrysophanol (0 µM,5 µM and 10 µM) for 24 h. Total RNA from the culture cells was isolated using the TRIzol Reagent, and reverse transcribed into cDNA using a kit. Target gene expression was analyzed by RT-PCR using Power SYBR Green PCR Master Mix, with GAPDH as the internal control. The forward and reverse primers were synthesized by Sangon (Shanghai, China) according to previously published sequences. The relative expression of the target genes was calculated using the2 -ΔΔCt method.
2.12. NLRP3 small interfering RNA (siRNA) transfection NLRP3 si RNA (sense: 5 -CCUACCUUCUCUAUCAGAUTT -3;anti: 5 -AUCUGAUAGAGAAGGUA GGTT -3), NLRP3 siRNA was purchased from GenePharma (Shanghai, China).NLRP3 siRNA (100 nM) or negative control siRNA was mixed with Lipofectamine 2000. The cells were incubated with the transfection mixture for 6 h and then changed with MEM medium with 10% FBS. The cells were incubated for an additional 48 h before harvest.

Binfen H., Zhao L., & Deng M. (2021). Chrysophanol Inhibits the Progression of Gastric Cancer by Activating NLRP3.

+ Open protocol

+ Expand

Investigating NLRP3 Inflammasome Activation

Check if the same lab product or an alternative is used in the 5 most similar protocols

E- cadherin (24E10), caspase-1 (D7F10), NLRP3 (D4D8T), vimentin (D21H3), GAPDH (D16H11) and cleaved caspase-1 p20 (Asp297) (D57A2) antibodies were purchased from Cell Signalling (Danvers, MA, USA). Antibody against IL-1β (AF5103) was purchased from Affinity Biosciences (Cincinnati, OH, USA). Antibodies against IL-17A were purchased from Servicebio (Wuhan, China). α-SMA antibody and HRP-conjugated goat anti-rabbit secondary antibody was purchased from Absin (Shanghai, China). Recombinant human IL-17A was acquired from MedChemExpress (Princeton, NJ, USA), and 0.1% bovine serum albumin (BSA) was reconstituted in phosphate buffered saline (PBS). NLRP3 siRNA and the negative control were synthesized by GenePharma (Shanghai, China). The sequences targeting NLRP3 used in our experiments were as follows: 5′-CAACAGGAGAGACCUUUAUTT-3′ and antisense, 5′-AUAAAGGUCUCUCCUGUUGTT-3′. For the transfection of siNLRP3, Lipofectamine 3000 reagent (Invitrogen CA, USA) was used.

Liu W., Xin M., Li Q., Sun L., Han X, & Wang J. (2022). IL-17A Promotes the Migration, Invasion and the EMT Process of Lung Cancer Accompanied by NLRP3 Activation. BioMed Research International, 2022, 7841279.

+ Open protocol

+ Expand

Schwann Cell Isolation and Manipulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Schwann cells were separated according to the previous report (Cobianchi et al., 2017 (link)). The Schwann cells were isolated from the site of sciatic nerve injury and 5 mm at the distal end at 48 h after sciatic nerve injury. Schwann cells were cultured under the condition of 37°C and 5% CO2 in Schwann cell specific medium. The MSTRG.24008.1 cDNA was synthesized by GenScript Biotech (Nanjing, China). The MSTRG.24008.1 small interfering RNA (siRNA), miR-331-3p agomir, miR-331-3p antagomirs, NLRP3 siRNA, MAL siRNA, and their respective controls were synthesized by GenePharma Co. (Shanghai, China). Vectors expressing these nucleic acids were transfected into Schwann cells using Lipofectamine 2000 (Invitrogen, United States).

Yin G., Peng Y., Lin Y., Wang P., Li Z., Wang R, & Lin H. (2021). Long Non-coding RNA MSTRG.24008.1 Regulates the Regeneration of the Sciatic Nerve via the miR-331-3p–NLRP3/MAL Axis. Frontiers in Cell and Developmental Biology, 9, 641603.

+ Open protocol

+ Expand

Silencing NLRP3 in Mouse Microglia

Check if the same lab product or an alternative is used in the 5 most similar protocols

Small interfering RNA (siRNA) targeted at mouse NLRP3 was used to silence NLRP3. The NLRP3 siRNA and nonsense control (NC) were synthesized by Gene Pharma (Suzhou, China). The sequence of siRNA is 5′-CGGCCUUACUUCAAUCUGUTT-3′ (forward) and 5′-ACAGAUUGAAGUAAGG CCGTT-3′ (reverse). The sequence of nonsense control siRNA is 5′-UUCUCCGAACGUGUCACGUTT-3′ (forward) and 5′-ACGUGACACGUUCG GAGAATT-3′ (reverse). Briefly, microglial cells were seeded in 24-well plates and cultured in complete culture medium without streptomycin/penicillin. Cells were transiently transfected with NLRP3 siRNA or negative control using Lipofectamine 3000 (Invitrogen). After transfection for 24 hours, cells were stimulated with curcumin (12.5 μM) or LPS (100 ng/mL) for 24 hours, followed by ATP (1 mM) for another 3 hours.

Ran Y., Su W., Gao F., Ding Z., Yang S., Ye L., Chen X., Tian G., Xi J, & Liu Z. (2021). Curcumin Ameliorates White Matter Injury after Ischemic Stroke by Inhibiting Microglia/Macrophage Pyroptosis through NF-κB Suppression and NLRP3 Inflammasome Inhibition. Oxidative Medicine and Cellular Longevity, 2021, 1552127.

+ Open protocol

+ Expand

Silencing NLRP3 in Human Endothelial Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

NLRP3 siRNA was purchased from Genepharma (Shanghai, China). HUVECs were transfected with the target siRNAs or the negative control siRNAs with Lipofectamine 2000 (Invitrogen, USA), referring to the manufacturer’s instruction. All experiments were performed after transfection for 24 h.

	Sequence (5′–3′)	Sequence (3′–5′)
NLRP3-homo-978	CAACAGGAGAGACCUUUAUTT	AUAAAGGUCUCUCCUGUUGTT
NLRP3-homo-2565	GCUGCUGAAAUGGAUUGAATT	UUCAAUCCAUUUCAGCAGCTT
NLRP3-homo-3449	GUGCGUUAGAAACACUUCATT	UGAAGUGUUUCUAACGCACTT

Feng Y.H., Li L.F., Zhang Q., Zhang J.H., Huang Y., Lv Y.L., Jia J.Z., Zhang D., Hu J.Y, & Huang Y.S. (2021). Microtubule associated protein 4 (MAP4) phosphorylation reduces cardiac microvascular density through NLRP3-related pyroptosis. Cell Death Discovery, 7, 213.

+ Open protocol

+ Expand

Silencing Key Regulators in Airway Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

VDR siRNA (1103; Sense: 5′‐CCUGCUCAGAUCACUGUAUTT‐3′, Antisense: 5′‐AUACAGUGAUCUGAGCAGGTT‐3′), NLRP3 siRNA (2999; Sense: 5′‐GGACCUCAGUGACAAUUCUTT‐3′, Antisense: 5′‐AGAAUUGUCACUGAGGUCCTT‐3′), Casp1 siRNA (918; Sense: 5′‐GGUGUGGUUUAAAGAUUCATT‐3′, Antisense: 5′‐UGAAUCUUUAAACCACACCTT‐3′), BRCC3 siRNA (279; Sense: 5′‐GGACCGAGUAGAAAUUUCUTT‐3′, Antisense: 5′‐AGAAAUUUCUACUCGGUCCTT‐3′) or negative control siRNA were obtained from GenePharma (Shanghai, China). For transfection, 80–100 nmol siRNA combined with 4 μl Lipofectamine RNAiMAX were incubated with HBE or HBE‐N cells in Opti‐MEM^® I reduced serum medium (31985070, Gibco) for 5–7 h as described before.⁴⁰ Cells were subsequently washed and incubated with fresh RPMI‐1640 for a further 24 h. Next, cells were harvested for evaluation of target protein expression or incubated with specific reagents. At the end of the incubation period, cells were harvested and subjected to western blot and other analyses.

Chen M., He Y., Hu X., Dong X., Yan Z., Zhao Q., Li J., Xiang D., Lin Y., Song H, & Bian X. (2023). Vitamin D3 attenuates SARS‐CoV‐2 nucleocapsid protein‐caused hyperinflammation by inactivating the NLRP3 inflammasome through the VDR‐BRCC3 signaling pathway in vitro and in vivo. MedComm, 4(4), e318.

+ Open protocol

+ Expand

Silencing NLRP3 Regulates Osteoblast Differentiation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Negative control (NC) siRNA and NLRP3 siRNA were purchased from GenePharma (Shanghai, China). siRNA was transfected into primary calvarial osteoblasts using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA) following the manufacturer's instructions; then, osteoblast differentiation was performed.

Chen W., Tang P., Fan S, & Jiang X. (2022). A Novel Inhibitor INF 39 Promotes Osteogenesis via Blocking the NLRP3/IL-1β Axis. BioMed Research International, 2022, 7250578.

+ Open protocol

+ Expand

Silencing NLRP3 in Neonatal Rat Cardiac Fibroblasts

Check if the same lab product or an alternative is used in the 5 most similar protocols

Primary neonatal rat cardiac fibroblasts were cultured with serum-free medium and subsequently transfected with NLRP3 siRNA (GenePharma). The sequence of siRNA duplexes targeting NLRP3 was as follows: sense 5′-CCAGGAGAGAACUUCUUAUTT-3′, anti-sense 5′-AUA AGAAGUUCUCUCCUGGTT-3′. After 6 h incubation, the media containing siRNA was replaced by DMEM containing 10 % FBS.

Zhang Z.Y., Dang S.P., Li S.S., Liu Y., Qi M.M., Wang N., Miao L.F., Wu Y., Li X.Y., Wang C.X., Qian L.L, & Wang R.X. (2022). Glucose Fluctuations Aggravate Myocardial Fibrosis via the Nuclear Factor-κB-Mediated Nucleotide-Binding Oligomerization Domain-Like Receptor Protein 3 Inflammasome Activation. Frontiers in Cardiovascular Medicine, 9, 748183.

+ Open protocol

+ Expand

Knockdown and Overexpression of Key Genes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Knockdown of PINK1, MMP1, NLRP3, and HSF1 was performed by reverse transfection of PINK1 siRNA (GenePharma, Shanghai, China), MMP1 siRNA (GenePharma), NLRP3 siRNA (GenePharma), and HSF1 siRNA (GenePharma). RBM3-overexpression plasmid pGV141-RBM3 was purchased from GeneChem (Shanghai, China). The cells were placed into the chambers of a 2-D clinostat 24 h after being transfected with the abovementioned siRNA or plasmid by using Lipofectamine 2000 (11668-019, Invitrogen, Waltham, Massachusetts, United States).

Li C., Pan Y., Tan Y., Wang Y, & Sun X. (2022). PINK1-Dependent Mitophagy Reduced Endothelial Hyperpermeability and Cell Migration Capacity Under Simulated Microgravity. Frontiers in Cell and Developmental Biology, 10, 896014.

+ Open protocol

+ Expand

Silencing NLRP3 in Hepatic Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

NLRP3 siRNA and a negative control (NC) were purchased from Genepharm Company (Shanghai, China). The isolated hepatic cells were transfected with NC siRNA or NLRP3 siRNAs using Lipofectamine 3000 Reagent (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA): siRNA 1 (5′-3′): GGCUAUGUACUAUCUGCUA; siRNA 2 (5′-3′): GGAUCUUUGCAGCGAUCAA; siRNA 3 (5′-3′): GGAUAGGUUUGCUGGGAUA; NC: GAGAUCUGCUUAGAUCGCA.

Su Q., Kuang W., Hao W., Liang J., Wu L., Tang C., Wang Y, & Liu T. (2021). Antituberculosis Drugs (Rifampicin and Isoniazid) Induce Liver Injury by Regulating NLRP3 Inflammasomes. Mediators of Inflammation, 2021, 8086253.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!