NCI‐H292 cells, a human lung mucoepidermoid carcinoma cell line, were purchased from the Korean Cell Line Bank (KCLB). The cell medium contained RPMI 1640 (Welgene), 10% fetal bovine serum (FBS) (Gibco), and 1% (w/v) antibiotic penicillin/streptomycin (Hyclone) for cell growth. Cells were cultured in a T75 cell culture flask in a CO2 incubator at 5% CO2/air‐humidified atmosphere at 37°C. For serum deprivation, confluent cells (5 × 105 cells/well in a six‐well plate) were washed twice with PBS and recultured in RPMI 1640 with 0.2% fetal bovine serum for 24 h. RAW264.7 murine macrophage cells were purchased from the Korean Cell Line Bank. Cells were cultured in Dulbecco's modified Eagle medium (Welgene) containing 10% fetal bovine serum (FBS) (Gibco) and 1% penicillin/streptomycin (Corning). RAW264.7 murine macrophage cells were maintained in a 5% CO2 humidified chamber at 37°C.
Rpmi 1640
Manufactured by Korean Cell Line Bank
RPMI-1640 is a widely used cell culture medium that provides a balanced salt solution and essential nutrients for the growth and maintenance of a variety of cell types, including human and animal cell lines.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640, by Welgene (2 mentions)
Rpmi 1640, by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Corning (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Nci n87, by Korean Cell Line Bank (1 mentions)
Mkn28, by Korean Cell Line Bank (1 mentions)
Dovitinib, by Selleck Chemicals (1 mentions)
Regorafenib, by Selleck Chemicals (1 mentions)
Entrectinib, by Selleck Chemicals (1 mentions)
Cabozantinib, by Selleck Chemicals (1 mentions)
Dovitinib lactate, by Selleck Chemicals (1 mentions)
Dovitinib dilactic acid, by Selleck Chemicals (1 mentions)
Crizotinib, by Selleck Chemicals (1 mentions)
Snu 16, by Korean Cell Line Bank (1 mentions)
Mkn 7, by Korean Cell Line Bank (1 mentions)
Loxo 101, by Selleck Chemicals (1 mentions)
Lncap, by Korean Cell Line Bank (1 mentions)
Du145, by Korean Cell Line Bank (1 mentions)
Fetal bovine serum (fbs), by Korean Cell Line Bank (1 mentions)
Recombinant rat ifnγ, by R&D Systems (1 mentions)
6 protocols using rpmi 1640
1
Culturing Human Lung Carcinoma and Murine Macrophage Cells
2
GC Cell Line Maintenance and Compound Testing
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The human GC cell lines NCI-N87, SNU16, MKN7, MKN28, and AGS were obtained from the Korean Cell Line Bank (Seoul, Republic of Korea) and maintained in RPMI-1640 supplemented with 10% fetal bovine serum. The cells were cultured at 37 °C with 100% humidity and 5% CO2. LOXO-101, entrectinib, dovitinib, dovitinib lactate, dovitinib dilactic acid, regorafenib, cabozantinib, and crizotinib were purchased from Selleck Chemicals (Houston, TX, USA).
3
Culturing Human Prostate Cancer Cells
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Human PCa cells (DU145 and LNCaP; Korean Cell Line Bank) were maintained in RPMI-1640 medium containing 10% fetal bovine serum (FBS). During experimentation, cells were grown in RPMI-1640 (Gibco; Thermo Fisher Scientific, Inc.) containing 1% FBS. Cells were grown at 37˚C and 5% CO2 in a humidified incubator.
4
Evaluation of LLT and LS803 on Lung Epithelial Cells
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NCI-H292 human lung epithelial cells were obtained from the Korean Cell Line Bank (Korea), and cultured in RPMI-1640 medium containing 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin. RPMI-1640, FBS, and antibiotics were purchased from Welgene. The cells were seeded into 48-well cell culture plates at a density of 8 × 104 cells/well and cultured for 24 h, followed by starvation in RPMI-1640 medium containing 0.2% FBS at 37°C for 24 h. The cells were then co-treated with LLT or LS803 while stimulated with 2% CSE and 100 ng/ml LPS. Post-treatment, the culture supernatants were collected and the cytokine levels were measured using the enzyme-linked immunosorbent assay (ELISA).
5
T47D Cell Culture Protocol
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Cell culture. T47D cells were obtained from the Korean Cell Line Bank (KCLB; Seoul, Republic of Korea) and cultured in RPMI 1640 (Cat. No. LM 011-01) supplemented with 10% fetal bovine serum (Cat. No. S 001-01) and 0.5× penicillin-streptomycin (Cat. No. LS 202-02) at 37˚C in a humidified atmosphere with 5% CO 2 . These culture reagents and the cultureware (Cat. Nos. 20101, 30012, and 30096) were purchased from WELGENE (Gyeongsan-si, Republic of Korea) and SPL Life Sciences (Pocheon-si, Republic of Korea), respectively. Under MTN, medium used to seed cells was maintained until an experiment for analysis as in conventional cell culture experiments, while under EXC, the medium was replaced with fresh medium every day until the experiment (a total of three times).
6
Regulation of Pancreatic β-Cell Signaling
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Recombinant rat IFN-γ was obtained from R&D Systems (Minneapolis, MN, USA). JAK inhibitor I (a pan-JAK inhibitor) and LY294002 (a PI3K/PKB inhibitor) were obtained from Calbiochem (Billerica, MA, USA) and Biomol (Plymouth Meeting, PA, USA), respectively. Anti-p-STAT-1 (#9171), anti-STAT-1 (#9175), anti-p-PKB (#9271) and anti-PKB (#9272) antibodies were purchased from Cell Signaling Technology (Boston, MA, USA). β-����� �������� (A5441) was purchased from Sigma (St. Louis, MO, USA). PCR primers were purchased from Bioneer (Daejeon, Korea). RPMI-1640 was purchased from Gibco-BRL (Carlsbad, CA, USA). Other chemicals, including SFN, were obtained from Sigma.
Cell culture. The INS-1 cells, an immortalized rat pancreatic β-cell line (Korean Cell Line Bank, Seoul, Korea), were cultured in RPMI-1640 medium containing 11.2 mM glucose, 2 mM l-glutamine, 10% heat-inactivated fetal bovine serum, 1 mM pyruvate, 10 mM HEPES, 50 µM 2-mercaptoethanol and 100 µg/ml streptomycin.
Cell culture. The INS-1 cells, an immortalized rat pancreatic β-cell line (Korean Cell Line Bank, Seoul, Korea), were cultured in RPMI-1640 medium containing 11.2 mM glucose, 2 mM l-glutamine, 10% heat-inactivated fetal bovine serum, 1 mM pyruvate, 10 mM HEPES, 50 µM 2-mercaptoethanol and 100 µg/ml streptomycin.
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