Rzx10 lux

Manufactured by Tucker-Davis Technologies

The RZX10 LUX is a piece of lab equipment designed for measuring light intensity. It provides accurate and reliable measurements of luminous flux, illuminance, and related light parameters. The device features a compact and durable design suitable for use in various laboratory settings.

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Lab products found in correlation

Synapse software, by Tucker-Davis Technologies (2 mentions) Digital optical power meter, by Thorlabs (2 mentions) Minicube, by Doric (2 mentions)

2 protocols using rzx10 lux

In-Vivo Fiber Photometry Recordings in Mice

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We anesthetized animals using 2% isoflurane and placed them in a stereotaxic frame. Mice were implanted with a fiber optic probe (400 μM diameter, 0.39NA; RWD Life Sciences) in the PBN (−5.2mm AP, +1.5mm ML, −2.2 to −2.5mm DV) during the same surgery they were injected with the viral construct and the head plate was implanted. The mice were given 3 weeks to recover and to allow for viral expression in their home cage.
For recordings, the fiber optic probe was connected to an RZX10 LUX fiber photometry processor running Synapse software (Tucker-Davis Technologies) through a Doric mini cube (Doric Lenses). LEDs at 465 nm and 405 nm were used for GCaMP excitation and isosbestic control, respectively. LED power was calibrated weekly using a digital optical power meter (Thor Labs).

Smith J.A., Ji Y., Lorsung R., Breault M.S., Koenig J., Cramer N., Masri R, & Keller A. (2023). Parabrachial nucleus activity in nociception and pain in awake mice. bioRxiv.

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Fiber Photometry Recordings in Mouse Parabrachial Nucleus

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Mice were anesthetized with an intraperitoneal injection of 2 mg/kg urethane, placed on a stereotaxic frame and a fiber optic probe (400 μm diameter, 0.39 NA; RWD Life Sciences) was placed in the right PB at the same coordinates used for NE2h injection (−5.2 mm AP, +1.5 mm ML, −2.2 to −2.5 mm DV). The fiber optic probe was connected to a RZX10 LUX fiber photometry processor running Synapse software (Tucker-Davis Technologies) through a Doric mini cube (Doric Lenses). LEDs at 465 nm (30 μW) and 405 nm (10 μW) were used for NE2h excitation and isosbestic control respectively. LED power was verified and calibrated as needed using a digital optical power meter (Thor Labs). The probes caused minimal tissue damage which was undetectable below the superficial inferior colliculus. Where present, we used this superficial damage to verify our recordings were in the correct rostrocaudal and mediolateral planes above PB.

Ji Y., Onwukwe C., Smith J., Laub H., Posa L., Keller A., Masri R, & Cramer N. (2023). Noradrenergic Input from Nucleus of the Solitary Tract Regulates Parabrachial Activity in Mice. eNeuro, 10(5), ENEURO.0412-22.2023.

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