Neochlorogenic acid, gallic acid, protocatechuic acid, chlorogenic acid, caffeic acid, p-hydroxybenzoic acid, 1,3-dicaffeoylquinic acid, p-coumaric acid, rutin, quercetin 3-β-galactoside, ferulic acid, taxifolin, 3,4-dicaffeoylquinic acid, trans-m-coumaric acid, quercetin 3-α-L-rhamnoside, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, rosmarinic acid, myrcetin, luteolin, quercetin, trans-cinnamic acid, apigenin, kaempferol, fenofibrate, dimethyl sulfoxide (DMSO), ethylenediaminetetraacetic acid (EDTA), hematoxylin, eosin, and trizma base were purchased from Sigma-Aldrich (St. Louis, MO, USA). Fetal bovine serum (FBS), Dulbecco’s modified Eagle’s medium (DMEM), streptomycin, and penicillin were purchased from Invitrogen (Carlsbad, CA, USA). Other reagents were commercially available and of special grade.
4 5 dicaffeoylquinic acid
Manufactured by Merck Group
Sourced in United States, Switzerland, Germany, Malaysia
4,5-dicaffeoylquinic acid is a chemical compound that can be used as a laboratory standard. It is a naturally occurring compound found in various plants. The product provides a reference standard for analytical and research applications.
Automatically generated - may contain errors
Lab products found in correlation
3 5 dicaffeoylquinic acid, by Merck Group (4 mentions)
3 4 dicaffeoylquinic acid, by Merck Group (3 mentions)
Quercetin, by Merck Group (3 mentions)
Chlorogenic acid, by Merck Group (3 mentions)
Apigenin, by Merck Group (2 mentions)
Neochlorogenic acid, by Merck Group (2 mentions)
Luteolin, by Merck Group (2 mentions)
Gallic acid, by Merck Group (2 mentions)
Kaempferol, by Merck Group (2 mentions)
Taxifolin, by Merck Group (1 mentions)
Myrcetin, by Merck Group (1 mentions)
Rosmarinic acid, by Merck Group (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Hematoxylin, by Merck Group (1 mentions)
Trans cinnamic acid, by Merck Group (1 mentions)
Ethylenediaminetetraacetic acid edta, by Merck Group (1 mentions)
1 3 dicaffeoylquinic acid, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Trizma base, by Merck Group (1 mentions)
4 protocols using 4 5 dicaffeoylquinic acid
1
Comprehensive Phytochemical Profiling
2
Standardized Antioxidant Compound Preparation
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Water was purified using a Milli–Q system (Millipore, Bedford, MA, USA). Ethanol (96%) was obtained from Vilniaus degtine (Vilnius, Lithuania). Anhydrous acetic acid (99.8%), and hydrochloric acid (37%) were purchased from Sigma-Aldrich (Buchs, Switzerland). The following reagents were used: 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and diammonium salt (ABTS). The following standards were used: 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), quercetin, chlorogenic acid, apigenin, 4-o-caffeoylquinic acid, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, neochlorogenic acid, kaempferol-3-rutinoside, and ellagic acid from Sigma-Aldrich (Buchs, Switzerland) and santin from PlantMetaChem (Giesen, Germany). All the reagents and standards were of analytical grade. The stock solutions of phenolic compounds were prepared in 96% ethanol.
3
Quantification of Chlorogenic Acid Derivatives
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HPLC-grade standard substances, namely neochlorogenic acid (5-caffeoylquinic acid), chlorogenic acid (3-caffeoylquinic acid), cryptochlorogenic acid (4-dicaffeoylquinic acid), 4,5-dicaffeoylquinic acid, 1,5-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid and 3,4-dicaffeoylquinic acid were purchased from Sigma–Aldrich GmbH (Steinheim, Germany). Solvents and other substances were obtained as follows: acetonitrile (purity 99.9%) and methanol (purity 99.9%) from Sigma-Aldrich (Steinheim, Germany); trifluoroacetic acid (purity 99.8%) supplied from Merck (Darmstadt, Germany); 2,2’-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) di-ammonium salt (ABTS) (purity ≥ 98%) and potassium persulphate (purity ≥ 99%) purchased from Sigma (St. Louis, MO, USA); Trolox (purity ≥ 98%) received from Fluka Chemika (Buchs, Switzerland). Ultrapure water was purified using a Millipore water-cleaning system (Bedford, MA, USA).
4
Quantifying Phenolic Acids in Samples
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Ultra-high performance liquid chromatography (UHPLC, 1290 Infinity Quaternary LC System, Agilent, Santa Clara, CA, USA) was used to separate and identify the phenolic acids. The chromatographic system conditions were set as follows: mobile phase, 0.03 M orthophosphoric acid (A) and methanol HPLC grade (B); detector, UV 280-360 nm; column, C18 column (5.0 μm, 4.6 mm inner diameter [ID] × 250 mm); column oven temperature, 35 °C; and flow rate, 1.0 mL/min. Gradient elution was performed as follows: 0–10 min, 10 % B; 10–15 min, 50 % B; 15–20 min, 100 % B; and finally 5 min for washing. All standards (quercetin, luteolin, myricetin, kaempferol, gallic acid, chlorogenic acid, 3,5-Di-caffeoylquinic acid and 4,5-Di-caffeoylquinic acid were purchased from Sigma-Aldrich (M) Sdn Bhd, Selangor, Malaysia) were dissolved in methanol HPLC grade. Linear regression equations were calculated using Y = aX ± b, where X is the concentration of the related compound and Y the peak area of the compound obtained from UHPLC. The linearity was established by the coefficient of determination (R2). UHPLC analysis was performed in triplicate and values are the average of three replicates.
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