Cis 3 hexenyl acetate

Cyclohexanol (>98.0%) and cis-3-hexen-1-ol (97.0 + %) were obtained from Fujifilm Wako Pure Chemical Corporation (Osaka, Japan). Palmitic acid was acquired from Nacalai Tesque, Inc. (Kyoto, Japan). trans-2-Decenal (>93.0%), trans-2-heptenal (>95.0%), trans-2-hexen-1-ol (>95.0%), trans-2-hexenal (>97.0%), cis-3-hexenyl acetate (>97.0%), and hexyl acetate (>99.0%) were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). The other reagents were described in our previous paper.^{11 (link)} The impurities derived from extraction solvents (hexane, methanol, and dichloromethane) for OA-LLE, SAFE, and HS-SPME were not observed. The monovarietal extra virgin olive oils (EVOO), Hojiblanca produced in Andalucía (Spain) and Mission and Lucca produced in Kagawa (Japan), were used in this study. These EVOOs were purchased from a market in Japan and stored at −20 °C until used.

In the manner described above, strawberries and 1 μmol trans-2-hexenal were incubated in 120-mL plastic cups for 24 h. Hexane was used as a control. After washing with detergent and a 70% ethanol spray, fruit were placed in a clear sampling bag (GL Sciences Inc., Tokyo, Japan). The bag was left standing for 2 h at room temperature. A Tenax TA Adsorbent Resin (60/80) (GERSTEL GmbH & Co., Mülheim an der Ruhr, Germany) was attached to the sampling bag, and 200 mL of the gas in the bag was collected by pulling the suction pump. Tenax TA-absorbed gas was heat-desorbed using TDS/CIS (GERSTEL GmbH & Co.). Collected gas was analysed using an Agilent 6890/5973 A GC–MS system (Agilent Technologies, Inc., Santa Clara, CA, USA). An InertCap Pure-WAX column (GL Sciences Inc.) was used. The temperature was increased from 50 °C to 180 °C at a rate of 5 °C per min, and then to 250 °C at a rate of 20 °C per min. The temperature was held at 250 °C for 10 min. The ionization voltage was 70 eV. The m/z was measured from 35 to 400. Obtained data were analysed using Agilent Masshunter Qualitative Analysis B.07.00 software. trans-2-Hexenyl acetate, cis-3-hexenyl acetate, and hexyl acetate were purchased from Tokyo Chemical Industry Co., LTD.