Sephadex LH 20 (Pharmacia, Stockholm, Sweden) and Diaion HP-20 AG were used for column chromatography (CC). Thin-layer chromatography (TLC) was performed on silica gel GF254 precoated plates (Fluka, Steinheim, Germany) using the following solvent systems: S1, CH2Cl2/methanol (90:10), S2, CH2Cl2/methanol (80:20), S3, ethyl acetate/methanol/water/formic acid (100:16.5:13.5:2.5). The chromatograms were visualized under UV (at 254 and 366 nm) before and after exposure to ammonia vapour and spraying with AlCl3. Dibenzylfluorescein, NaOH, NADPH, glucose 6-phosphate, glucose 6-phosphate dehydrogenase, MgCl2, and potassium phosphate (pH 7.4) were obtained from Sigma Chemical Co., (St Louis, MO 63103 USA). Aromatase enzyme (CYP19, BD Biosciences, San Jose, CA). Estradiol benzoate, Folone ampoules, Misr Co. for Pharm. Ind. S.A.E. and standard genistein “Adipogen Life Sciences Inc., Germany”.
Dibenzylfluorescein
Manufactured by Merck Group
Sourced in United States
Dibenzylfluorescein is a fluorescent dye used in laboratory settings as a reagent for analytical and detection purposes. It is a chemical compound that exhibits fluorescent properties when exposed to specific wavelengths of light.
Automatically generated - may contain errors
Lab products found in correlation
Potassium phosphate, by Merck Group (1 mentions)
Mgcl2, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
Glucose 6 phosphate (g6p), by Merck Group (1 mentions)
Glucose 6 phosphate dehydrogenase, by Merck Group (1 mentions)
Nadph, by Merck Group (1 mentions)
Chrysin, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Avantor (1 mentions)
4 2 hydroxyethyl 1 piperazineethanesulfonic acid hepes, by Avantor (1 mentions)
Thiazolyl blue tetrazolium blue mtt, by Merck Group (1 mentions)
Ultra clear centrifuge tube, by Beckman Coulter (1 mentions)
Quercitrin, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Quercetin, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Acetic acid, by RCI Labscan (1 mentions)
Potassium chloride (kcl), by Merck Group (1 mentions)
2 protocols using dibenzylfluorescein
1
Chromatographic Purification and Aromatase Inhibition
2
Biochemical Assay Reagents Procurement
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Chrysin, quercetin, quercitrin, dibenzylfluorescein, and thiazolyl blue tetrazolium blue (MTT) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Potassium chloride (KCl) and methanol were obtained from Merck (Darmstadt, Germany). Acetic acid was obtained from RCI Labscan (Bangkok, Thailand). 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) was purchased from AMRESCO (Solon, OH, USA). Nicotinamide adenine dinucleotide phosphate (NADPH) was obtained from Tocris (Ellisville, MO, USA). Antibiotic-antimycotic Dulbecco's modified Eagle's medium (DMEM) and fetal bovine serum (FBS) were obtained from Gibco BRL Life Technologies (Grand Island, NY, USA). Dimethyl sulfoxide (DMSO) was obtained from Amresco (Salon, OH, USA). All solvents were of analytical grade. Ninety-six-well plates were obtained from Costar (Cambridge, MA, USA). Ultra-clear centrifuge tubes were purchased from Beckman Coulter Inc. (Brea, CA, USA).
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