Polaris instrument

Manufactured by Akoya Biosciences

The Polaris instrument is a multi-functional platform that enables high-plex spatial analysis of protein and RNA targets within intact tissue samples. The core function of the Polaris instrument is to facilitate the detection and quantification of multiple biomolecular targets simultaneously within a single tissue section.

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Lab products found in correlation

Ab26116, by Abcam (1 mentions) Ab290, by Abcam (1 mentions) Gad67, by Abcam (1 mentions) Pv235, by Swant (1 mentions)

2 protocols using polaris instrument

Analysis of Ovarian Tumor Images

Cited 2 times

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Images from 12 previously described ovarian tumors were analyzed [41 (link), 45 (link)]. Tumors were formalin-fixed, paraffin embedded (FFPE) and assembled in a tissue microarray (TMA) consisting of 2 mm cores Akoya Polaris instrument. TMA construction was approved by Colorado Multiple Institutional Review Board (#17–7788).

Ahmadian M., Rickert C., Minic A., Wrobel J., Bitler B.G., Xing F., Angelo M., Hsieh E.W., Ghosh D, & Jordan K.R. (2023). A platform-independent framework for phenotyping of multiplex tissue imaging data. PLOS Computational Biology, 19(9), e1011432.

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Multiplex IHC for Neuronal Markers

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IHC was performed on serial section slides in two independent multiplexed batches. The first batch followed the sequence mouse anticalcium/calmodulin-dependent protein kinase II alpha (MA1-048; Invitrogen), rabbit antisomatostatin (T-4102; Peninsula Laboratories), rabbit anti-GFP (ab290; Abcam), and mouse anti-PV (PV235; Swant). The second batch followed the sequence, mouse anti-glutamate decarboxylase (GAD67, ab26116; Abcam), mouse anti-fox-3 (NeuN, MAB 377; Millipore), and rabbit anti-GFP. After primary and secondary antibody incubation, slides were stained with DAPI. Whole slide images were captured at 20 × using an Akoya Biosciences Polaris instrument. Image exposures for each fluorochrome were constant for all slides. Detailed image analysis is described in the Supplementary Materials.

Tanenhaus A., Stowe T., Young A., McLaughlin J., Aeran R., Lin I.W., Li J., Hosur R., Chen M., Leedy J., Chou T., Pillay S., Vila M.C., Kearney J.A., Moorhead M., Belle A, & Tagliatela S. (2022). Cell-Selective Adeno-Associated Virus-Mediated SCN1A Gene Regulation Therapy Rescues Mortality and Seizure Phenotypes in a Dravet Syndrome Mouse Model and Is Well Tolerated in Nonhuman Primates. Human Gene Therapy, 33(11-12), 579-597.

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