Total RNA from the TRs at 50 DAT and 95 DAT were extracted using RNA plant kits (Tiangen, DP441-50T), and then reverse transcribed, and the synthetic cDNA was used for qPCR. In this study, each qPCR result had three biological replications. The primers in this study were shown in Supplementary Table S1 . The reference gene was GAPDH, and the relative expression levels related in sucrose-starch metabolism were analyzed using the 2−ΔΔCT method [45 (link),46 (link)].
Rna plant kits
Manufactured by Tiangen Biotech
Sourced in China
The RNA plant kits from Tiangen Biotech are designed to efficiently extract and purify high-quality RNA from plant samples. The kits utilize a specialized protocol and reagents to ensure effective isolation of RNA for downstream applications.
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Lab products found in correlation
2 protocols using rna plant kits
1
Quantification of Sucrose-Starch Metabolism
2
Transcriptomics of Cotton and Arabidopsis
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Plants of Gossypium hirsutum "CCRI 8" and G. barbadense "Pima90-53" were grown in an experimental field in Baoding, China. Ovules and epidermal fibers were collected at 0, 5, 10, 15, 20, 25, 30, 35, and 40 DPA, respectively, and were immediately immersed in liquid nitrogen.
Leaf, stem and root tissues Arabidopsis ("Columbia" ecotype, or "Col-O") seedlings were sampled from light chamber-grown (16 h light period, 23°C; 8 h dark period, 22°C; 70% relative humidity) and placed in liquid nitrogen.
Total RNAs were isolated from the ovules and fibers of both cotton cultivars with RNAplant Kits (TIANGEN, China), while RNA was isolated from Arabidopsis tissues with a TransZol Kit (TransGen Biotech, China). Purified RNA was treated with DNase I (Promega, USA), according to the manufacturer's instructions. Then, cDNA was synthesized using an M-MLV Kit (TaKaRa, China).
Leaf, stem and root tissues Arabidopsis ("Columbia" ecotype, or "Col-O") seedlings were sampled from light chamber-grown (16 h light period, 23°C; 8 h dark period, 22°C; 70% relative humidity) and placed in liquid nitrogen.
Total RNAs were isolated from the ovules and fibers of both cotton cultivars with RNAplant Kits (TIANGEN, China), while RNA was isolated from Arabidopsis tissues with a TransZol Kit (TransGen Biotech, China). Purified RNA was treated with DNase I (Promega, USA), according to the manufacturer's instructions. Then, cDNA was synthesized using an M-MLV Kit (TaKaRa, China).
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