The largest database of trusted experimental protocols

Chemidoc mp chemiluminescence imager

Manufactured by Bio-Rad
Sourced in United States

The ChemiDoc MP Chemiluminescence Imager is a high-performance imaging system designed for the detection and analysis of chemiluminescent signals. It captures images of gels, blots, and other samples that emit light as a result of a chemical reaction.

Automatically generated - may contain errors

2 protocols using chemidoc mp chemiluminescence imager

1

Extracellular Vesicle Protein Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
For western blotting detection, a total of 30 μg of T-EVs or crude protein extracted from cell lysates was separated by 12% SDS-PAGE and transferred to a polyvinylidene difluoride membrane (Millipore, Danvers, Massachusetts, USA). The membranes were blocked with 5% BSA in TBST and then incubated with appropriate primary antibodies overnight at 4 °C. After incubation with horseradish peroxidase-coupled secondary antibodies for 1 h, the membranes were scanned using a ChemiDoc MP Chemiluminescence imager (Bio-Rad, Hercules, California, USA) according to the manufacturer's instructions. For FCM analysis, 20 μg of EVs was incubated with 5 μL of 4-μm-diameter aldehyde/sulfate latex beads (Invitrogen) for 15 min at room temperature in PBS, with a final volume of 20 μL. The mixture was then transferred to 1 mL of PBS and incubated with gentle shaking for 1 h. After centrifugation, the pellet was blocked by incubation with 20 μL of fetal bovine serum for 30 min. EV-coated beads were washed three times in PBS and resuspended in 50 μL of PBS. Afterward, the beads were incubated with appropriate fluorophore-conjugated antibodies for 1 h at room temperature in the dark. The beads were analyzed by FCM (Beckman Coulter) 38 (link).
+ Open protocol
+ Expand
2

Phenotypic Screening of Algal Transformants

Check if the same lab product or an alternative is used in the 5 most similar protocols
For the large-scale phenotypic screening of transformants, 2 mL of a dense algal culture was concentrated on a 100 µm nylon screen and resuspended in 400 µL of assay buffer (0.1 M K2HPO4 pH 7.6, 0.5 M NaCl, 1 mM EDTA) [73 (link)]. The algae cells were then disrupted by 15 s of ultrasound sonication, using a Sonopuls HD2070 sonicator (Bandelin Electronic, Berlin, Germany) at 70% power, under cooling with ice. Based on the measured chlorophyll concentration of each sample, the samples were equalized for lysate concentration by adding assay buffer. In a black 96-well polystyrene plate (Thermo Fisher Scientific, Wilmington, DE, USA), 200 µL of each lysate was added to 50 µL of 10 µM coelenterazine (Fluka, Neu-Ulm, Germany) in assay buffer, using a multichannel pipette. Chemiluminescence was detected with a ChemiDoc MP chemiluminescence imager (Biorad, Hercules, CA, USA) and quantified using FIJI (ImageJ 1.51w) [78 (link)]. To document the uniformly adjusted lysate concentration of all samples based on their chlorophyll content, brightfield images were taken using a Nikon D200 camera.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!