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Paxgene

Manufactured by BD
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The PAXgene is a sample collection and stabilization system designed for the collection, transport, storage, and processing of blood RNA samples. It provides a standardized pre-analytical system to ensure the integrity of RNA from whole blood samples.

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Lab products found in correlation

Tempus, by Thermo Fisher Scientific (2 mentions) Maxwell 16 lev blood rna kit, by Promega (2 mentions) Maxwell extractor, by Promega (2 mentions) Ncounter analysis technology, by NanoString (2 mentions) Rneasy plus micro kit, by Qiagen (2 mentions) Paxgene blood rna kit, by Qiagen (2 mentions) Serum vacutainer tubes, by BD (1 mentions) Lithium heparin vacutainer tube, by BD (1 mentions) Blood rna tubes, by BD (1 mentions) Vacutainer k2 edta tubes, by BD (1 mentions) Advia 1800 chemistry system, by Siemens (1 mentions) Vacutainer, by BD (1 mentions) Globinclear kit, by Thermo Fisher Scientific (1 mentions) Agilent bioanalyzer, by Agilent Technologies (1 mentions) Nanodrop 1000 spectrophotometer, by Agilent Technologies (1 mentions)

7 protocols using paxgene

1

Transcriptome Analysis of COVID-19 Patients

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Whole blood was collected into PAXgene (BD Biosciences) or Tempus (Thermo Fisher Scientific) blood RNA tubes or EDTA tubes. RNA was extracted with the corresponding RNA extraction kits or with the Maxwell 16 LEV Blood RNA kit and a Maxwell extractor (Promega) and quantified by spectrometry (Nanovue). For P5, RNA was extracted from sorted T cells with the RNeasy Plus microkit (Qiagen). Relative expression levels were determined for TRBV 11–2 with nCounter analysis technology (NanoString Technologies), by calculating TRBV 11–2 mRNA levels relative to other TRBV mRNA levels and normalizing against the median value for the healthy volunteer group. Blood samples from P1, P2, and P5 were obtained on days 7, 4, and 9 after symptom onset, respectively.
Boyce F.M., Beggs A.H., Feener C, & Kunkel L.M. (1991). Dystrophin is transcribed in brain from a distant upstream promoter.. Proceedings of the National Academy of Sciences of the United States of America, 88(4), 1276-1280.
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2

Comprehensive Pig Blood Sample Collection Protocol

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At the weekly blood draws, approximately 2.5 mL of whole blood was collected into K2EDTA vacutainer tubes (BD Biosciences, Cat#367844), 2.0 mL into lithium heparin vacutainer tubes (BD Biosciences, Cat#367880, Franklin Lakes, NJ, USA), and 2.5 mL into blood RNA tubes (BD, PAXgene®, Cat#762165, Franklin Lakes, NJ, USA). In addition, using 2 drops of whole blood per piglet, 2 slide smears were collected. Plasma samples were obtained by spinning the lithium heparin tubes at 1500× g for 10 min at 4 °C. A total of 500 uL of plasma, K2EDTA tubes, and the blood smear samples were submitted to the Veterinary Medical Diagnostic Laboratory at Texas A&M University (College Station, TX, USA) for metabolic profiling, mineral panel, and complete blood count (CBC), respectively. Additionally, plasma samples were submitted to the Eurofins Craft Technologies Laboratory (Wilson, NC, USA) for plasma insulin measurements using a quantitative porcine insulin ELISA assay. At day 21, the blood (~45 mL) was collected into serum vacutainer tubes (BD Biosciences, Cat#368045, Franklin Lakes, NJ, USA) as well as into blood RNA tubes for further analysis. Serum tubes were incubated at room temperature for approximately 20 min and then centrifuged at 1500× g for 10 min at 4 °C. Serum aliquots were stored at −80 °C until further processing.
Rosa F., Yelvington B., Terry N., Tripp P., Pittman HE I.I.I., Fay B.L., Ross T.J., Sikes J.D., Flowers J.B., Bar-Yoseph F, & Yeruva L. (2022). Evaluation of the Safety of a Plant-Based Infant Formula Containing Almonds and Buckwheat in a Neonatal Piglet Model. Nutrients, 14(7), 1499.
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3

Transcriptome Analysis of COVID-19 Patients

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Whole blood was collected into PAXgene (BD Biosciences) or Tempus (Thermo Fisher Scientific) blood RNA tubes or EDTA tubes. RNA was extracted with the corresponding RNA extraction kits or with the Maxwell 16 LEV Blood RNA kit and a Maxwell extractor (Promega) and quantified by spectrometry (Nanovue). For P5, RNA was extracted from sorted T cells with the RNeasy Plus microkit (Qiagen). Relative expression levels were determined for TRBV 11–2 with nCounter analysis technology (NanoString Technologies), by calculating TRBV 11–2 mRNA levels relative to other TRBV mRNA levels and normalizing against the median value for the healthy volunteer group. Blood samples from P1, P2, and P5 were obtained on days 7, 4, and 9 after symptom onset, respectively.
Lee D., Pen J.L., Yatim A., Dong B., Aquino Y., Ogishi M., Pescarmona R., Talouarn E., Rinchai D., Zhang P., Perret M., Liu Z., Jordan I., Bozdemir S.E., Bayhan G.I., Beaufils C., Bizien L., Bisiaux A., Lei W., Hasan M., Chen J., Gaughan C., Asthana A., Libri V., Luna J.M., Jaffré F., Hoffmann H.H., Michailidis E., Moreews M., Seeleuthner Y., Bilguvar K., Mane S., Flores C., Zhang Y., Arias A.A., Bailey R., Schlüter A., Milisavljevic B., Bigio B., Voyer T.L., Materna M., Gervais A., Moncada-Velez M., Pala F., Lazarov T., Levy R., Neehus A.L., Rosain J., Peel J., Chan Y.H., Morin M.P., Pino-Ramirez R.M., Belkaya S., Lorenzo L., Anton J., Delafontaine S., Toubiana J., Bajolle F., Fumadó V., DeDiego M.L., Fidouh N., Rozenberg F., Pérez-Tur J., Chen S., Evans T., Geissmann F., Lebon P., Weiss S.R., Bonnet D., Duval X., Pan-Hammarström Q., Planas A.M., Meyts I., Haerynck F., Pujol A., Sancho-Shimizu V., Dalgard C.L., Bustamante J., Puel A., Boisson-Dupuis S., Boisson B., Maniatis T., Zhang Q., Bastard P., Notarangelo L., Béziat V., de Diego R.P., Rodriguez-Gallego C., Su H.C., Lifton R.P., Jouanguy E., Cobat A., Alsina L., Keles S., Haddad E., Abel L., Belot A., Quintana-Murci L., Rice C.M., Silverman R.H., Zhang S.Y, & Casanova J.L. (2023). Inborn errors of OAS–RNase L in SARS-CoV-2–related multisystem inflammatory syndrome in children. Science (New York, N.Y.), 379(6632), eabo3627.
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4

Maternal DNA and RNA Extraction

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DNA is extracted at baseline from maternal whole blood with appropriate steps of cell lysis, protein precipitation and washing. RNA is extracted from the maternal PAXGene samples (BD, Heidelberg, Germany) at baseline and follow-up. DNA and RNA are stored at −80°C.
Fritsche L., Hummel J., Wagner R., Löffler D., Hartkopf J., Machann J., Hilberath J., Kantartzis K., Jakubowski P., Pauluschke-Fröhlich J., Brucker S., Hörber S., Häring H.U., Roden M., Schürmann A., Solimena M., de Angelis M.H., Peter A., Birkenfeld A.L., Preissl H., Fritsche A, & Heni M. (2022). The German Gestational Diabetes Study (PREG), a prospective multicentre cohort study: rationale, methodology and design. BMJ Open, 12(2), e058268.
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5

Longitudinal Biomarker Monitoring in AECOPD

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After obtaining informed consent from the subjects, blood samples were collected in PAXgene® (Becton Dickinson, Franklin Lakes, NJ, USA), EDTA, and serum tubes on day 1 and day 3 of hospitalization, at discharge, and on day 30 and day 90 post-admission date. Blood components were processed as per standardized protocol and stored at −80°C until analysis. Serum CRP was measured via a high-sensitivity assay on the Advia® 1800 Chemistry System (Siemens Healthcare GmbH, Erlangen, Germany), in the Clinical Laboratory of St Paul’s Hospital (Department of Pathology and Laboratory Medicine, Vancouver, BC, Canada) following standard operating procedures. NT-proBNP was measured from EDTA whole blood specimens on the RAMP® 200 (Response Biomedical Corp, Vancouver, BC, Canada), which has a measurement range of 18–35,000 ng/L.
Baseline lung function measurements were performed at the time of convalescence (ie, at day 30 or day 90) for AECOPD patients. Spirometry was used to obtain lung function parameters after bronchodilator administration according to recommendations from American Thoracic Society/European Respiratory Society.14 (link)
Alotaibi N.M., Chen V., Hollander Z., Hague C.J., Murphy D.T., Leipsic J.A., DeMarco M.L., FitzGerald J.M., McManus B.M., Ng R.T, & Sin D.D. (2018). Phenotyping COPD exacerbations using imaging and blood-based biomarkers. International Journal of Chronic Obstructive Pulmonary Disease, 13, 217-229.
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6

Transcriptomic Analysis of PBMCs

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We isolated total RNA from PBMCs collected in PAXgene (Becton Dickinson) using a PAXgene Blood RNA Kit (Qiagen, Valencia, CA). An estimated 1.2 × 107 to 2.8 × 107 cells per sample were collected during blood sampling. We evaluated the quality of the isolated RNA, and performed cDNA synthesis. We prepared and sequenced libraries using a next-generation sequencer for bioinformatics analysis (RIKEN GENESIS, Tokyo, Japan). Using these isolated samples, we performed qPCR to confirm specific gene expression.
Miura M., Nishino M., Kawaguchi K., Li S., Shimakami T., Tamai T., Nakagawa H., Terashima T., Iida N., Takatori H., Arai K., Sakai Y., Yamashita T., Honda M., Kaneko S., Mizukoshi E, & Yamashita T. (2024). Programmed cell death-1 is involved with peripheral blood immune cell profiles in patients with hepatitis C virus antiviral therapy. PLOS ONE, 19(5), e0299424.
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7

Whole Blood RNA Extraction from Irradiated Mice

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Blood was collected from the mice 24 h after the onset of irradiation. Mice were rapidly euthanized by CO2 asphyxia and blood (approximately 0.5 ml) was collected by syringe (BD Vacutainer; Becton, Dickinson, Franklin Lakes, NJ) via cardiac puncture, mixed in PAXgene (Becton, Dickinson) blood RNA solution (1:4 ratio) and stored overnight at 4°C before further processing. The whole blood RNA was extracted using the PAXgene blood RNA kit (QIAGEN®, Valencia, CA) according to manufacturer’s instruction, followed by on-column DNase I treatment, and removal of globin transcripts using the GLOBINclear™ kit (Ambion®, Austin, TX) to remove both α- and β-globin transcripts. The RNA was quantified with a NanoDrop-1000 spectrophotometer, and quality was determined with the Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA). All RNA samples had RNA integrity numbers >9.0.
Paul S., Smilenov L.B., Elliston C.D, & Amundson S.A. (2015). Radiation Dose-Rate Effects on Gene Expression in a Mouse Biodosimetry Model. Radiation research, 184(1), 24-32.
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