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Dmi 6000 deconvolution microscope

Manufactured by Leica
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The Leica DMI 6000 Deconvolution Microscope is a high-performance imaging system designed for advanced microscopy applications. It features a motorized inverted microscope platform with a deconvolution module for enhanced image quality and resolution. The core function of the DMI 6000 is to capture and process digital images with improved clarity and detail, enabling researchers to obtain high-quality data for their scientific investigations.

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Lab products found in correlation

Hc pl apo 63 1.30 glyc corr cs2 objective, by Leica (2 mentions) Cfi plan apochromat λ dm 60 1.40 oil objective, by Nikon (2 mentions) Eclipse ti, by Nikon (2 mentions) Hc pl apo 63x 1.30 glyc corr cs2, by Leica (1 mentions) Huygens professional version 17, by Scientific Volume Imaging (1 mentions)

3 protocols using dmi 6000 deconvolution microscope

1

Fluorescence Imaging of ATRX Protein

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FISH and IF/FISH samples were imaged using a Leica DMI 6000 Deconvolution Microscope with the Leica HC PL APO 63×/1.30 GLYC CORR CS2 objective. Samples stained with TYPE 10-ATRX (740 nm) were imaged using Nikon Ti Eclipse with the Nikon CFI Plan Apochromat λ DM 60×/1.40 oil objective. Images were projected with maximum projection (3 µm; step size, 0.5 µm).
McHugh C.A., Chen C.K., Chow A., Surka C.F., Tran C., McDonel P., Pandya-Jones A., Blanco M., Burghard C., Moradian A., Sweredoski M.J., Shishkin A.A., Su J., Lander E.S., Hess S., Plath K, & Guttman M. (2015). The Xist lncRNA directly interacts with SHARP to silence transcription through HDAC3. Nature, 521(7551), 232-236.
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2

Fluorescence Imaging of ATRX Protein

Check if the same lab product or an alternative is used in the 5 most similar protocols
FISH and IF/FISH samples were imaged using a Leica DMI 6000 Deconvolution Microscope with the Leica HC PL APO 63×/1.30 GLYC CORR CS2 objective. Samples stained with TYPE 10-ATRX (740 nm) were imaged using Nikon Ti Eclipse with the Nikon CFI Plan Apochromat λ DM 60×/1.40 oil objective. Images were projected with maximum projection (3 µm; step size, 0.5 µm).
McHugh C.A., Chen C.K., Chow A., Surka C.F., Tran C., McDonel P., Pandya-Jones A., Blanco M., Burghard C., Moradian A., Sweredoski M.J., Shishkin A.A., Su J., Lander E.S., Hess S., Plath K, & Guttman M. (2015). The Xist lncRNA directly interacts with SHARP to silence transcription through HDAC3. Nature, 521(7551), 232-236.
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3

Multimodal Microscopy Imaging and Deconvolution

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DNA FISH/IF samples were imaged using a Leica DMI 6000
Deconvolution Microscope, with a z-stack collected for each channel (4
μm; step size, 0.2 μm). The objectives used were the
Leica HC PL APO 63x/1.30 GLYC CORR CS2 objective and the Leica HCX PL
APO 100X/1.40- 0.70na OIL objective. Samples were also imaged with a
ZEISS Laser Scanning Microscope (LSM) 800 with the ZEISS i
Plan-Apochromat 63x/1.4 Oil DIC M27 objective, with a z-stack collected
for each channel (step size, 0.37 μm). Deconvolution was
performed using Huygens Professional version 17.04 (Scientific Volume
Imaging, The Netherlands, software available at http://svi.nl) using the built-in theoretical point
spread function, the classic maximum likelihood estimation (CMLE)
algorithm, a signal to noise ratio of 20, and 50 iterations.
Quinodoz S.A., Ollikainen N., Tabak B., Palla A., Schmidt J.M., Detmar E., Lai M.M., Shishkin A.A., Bhat P., Takei Y., Trinh V., Aznauryan E., Russell P., Cheng C., Jovanovic M., Chow A., Cai L., McDonel P., Garber M, & Guttman M. (2018). Higher-order inter-chromosomal hubs shape 3D genome organization in the nucleus. Cell, 174(3), 744-757.e24.
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