Stemdiff neuron maturation kit

Manufactured by STEMCELL

The STEMdiff™ Neuron Maturation Kit is a laboratory product designed to facilitate the differentiation and maturation of neurons from stem cells. It contains a set of specialized media and supplements to support the various stages of neuronal development.

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Lab products found in correlation

Stemdiff neuron differentiation kit, by STEMCELL (2 mentions) Nestin, by BD (1 mentions) Neural induction medium, by STEMCELL (1 mentions) Matrigel with reduced growth factors, by Thermo Fisher Scientific (1 mentions) H9 wa09 cells, by WiCell (1 mentions) Aggrewell 800 plate, by STEMCELL (1 mentions) Mtesr1 medium, by STEMCELL (1 mentions) Accutase, by STEMCELL (1 mentions)

2 protocols using stemdiff neuron maturation kit

Tay-Sachs Patient NSCs Neuronalization

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Tay-Sachs patient NSCs were further differentiated into neurons using STEMdiff™ Neuron Differentiation Kit and STEMdiff™ Neuron Maturation Kit (StemCell Technologies) according to the manufacture’s protocol. After 7 days of differentiation of Tay-Sachs NSCs to neuronal precursors in 6- well plate using STEMdiff™ Neuron Differentiation medium, cells were dislodged and seeded into Poly-L-ornithine (PLO)/laminin coated black, clear bottom, tissue-culture treated 96-well plate in STEMdiff™ Neuron Maturation medium for another 7–14 days before downstream application. Immunofluorescence staining of several neuronal markers MAP2 (Cell signaling), beta-III-tubulin (Cell signaling), Neurofilament-L (Cell signaling) as well as Nestin (neural stem cell protein marker, BD Bioscience) were performed to characterize the neuronal cells derived from TSD patient and WT control NSCs.

Vu M., Li R., Baskfield A., Lu B., Farkhondeh A., Gorshkov K., Motabar O., Beers J., Chen G., Zou J., Espejo-Mojica A.J., Rodríguez-López A., Alméciga-Díaz C.J., Barrera L.A., Jiang X., Ory D.S., Marugan J.J, & Zheng W. (2018). Neural stem cells for disease modeling and evaluation of therapeutics for Tay-Sachs disease. Orphanet Journal of Rare Diseases, 13, 152.

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Directed Neural Differentiation of H9/WA09 Cells

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H9/WA09 cells (WiCell) were grown on Matrigel with reduced growth factors (ThermoFisher Scientific, #354230) in mTeSR1 medium (STEMCELL Technologies, #85850) at 37°C and 5% CO₂. Neural lineage differentiation was performed using STEMdiff™ neuron differentiation kit (STEMCELL Technologies, #08500) and STEMdiff™ neuron maturation kit (STEMCELL Technologies, #08510).ES cells were seeded onto AggreWell800 plates (STEMCELL Technologies, #34811) and fed with neural induction medium (STEMCELL Technologies, #05835) to form uni-sized embryoid bodies (EBs). On day 5, EBs were re-plated onto Matrigel-treated 6-well plates. Neural differentiation started from day 11 to day 16. On day 17, cells were treated with Accutase^® (STEMCELL Technologies) and seeded and fed with neuronal maturation medium till days 22–24. RNA samples were collected on days 10, 11, and 22 for deep-sequencing analysis. Cells were also seeded onto multi-chamber slides for immunofluorescence (IF) analysis.

Yang X., Xu B., Mulvey B., Evans M., Jordan S., Wang Y.D., Pagala V., Peng J., Fan Y., Patel A, & Peng J.C. (2019). Differentiation of human pluripotent stem cells into neurons or cortical organoids requires transcriptional co-regulation by UTX and 53BP1. Nature neuroscience, 22(3), 362-373.

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