Human embryonic kidney (293T/17, ATCC) cells were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% FBS and 5 mg/ml of sodium pyruvate. The following antibodies were used for immunoblotting analyses or immunohistochemistry: c-Myc, β-actin, and cleaved caspase-3 (Cell Signaling). BRD4, p21, p27, p57 and cyclinD1 (Santa Cruz). Additional reagents used in the study include: TransIT-LT1 transfection reagent (Mirus), puromycin, polybrene, fibronectin and 1xRIPA buffer (Sigma-Aldrich), enhanced chemiluminescence (ECL) detection reagent (Millipore) and staurosporine (Selleck Chemicals).
Enhanced chemiluminescence ecl detection reagent
Manufactured by Merck Group
Sourced in United States
The Enhanced chemiluminescence (ECL) detection reagent is a laboratory product designed to detect and quantify target proteins in Western blot analyses. The reagent utilizes a chemiluminescent reaction to generate a luminescent signal proportional to the amount of target protein present in the sample. This signal can be captured and analyzed using suitable detection equipment.
Automatically generated - may contain errors
Lab products found in correlation
Staurosporine, by Selleck Chemicals (1 mentions)
C myc, by Cell Signaling Technology (1 mentions)
Cleaved caspase 3, by Cell Signaling Technology (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
Polybrene, by Merck Group (1 mentions)
Puromycin, by Merck Group (1 mentions)
Transit lt1 transfection reagent, by Mirus Bio (1 mentions)
Anti e cadherin, by Wanlei (1 mentions)
Anti vimentin, by Wanlei (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
2 protocols using enhanced chemiluminescence ecl detection reagent
1
Characterization of 293T Cell Line
2
Immunodetection of Signaling Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Anti-Irisin (Human, Rat, Mouse, Canine specific) antibody was purchased from Phoenix Pharmaceuticals (CA, USA). Fluorescein isothiocyanate (FITC)-anti-rabbit IgG antibody was from BIOSS (Beijing, China). MTT was purchased from Sigma -Aldrich (MO, USA). Honchest33258 was purchased from Solarbio (Beijing, China). Anti-E-cadherin, anti-vimentin, and anti-cyclin D1 rabbit pAb were purchased from Wanleibio (Shenyang, China). Anti-total and anti-phosphorylated (Thr172) AMPK, anti-total and anti-phosphorylated (Ser2448) mTOR, anti-total and anti-phosphorylated (Thr389) p70S6 kinase (ribosomal protein S6 kinase), anti-total and anti-phosphorylated (Thr37/46) 4E-BP1 (eukaryotic translation initiation factor 4E binding protein 1), anti-beta actin rabbit antibodies, and anti-rabbit horseradish peroxidase (HRP)-conjugated IgG antibodies were obtained from Cell Signaling Technology (MA, USA). The enhanced chemiluminescence (ECL) detection reagent was from Millipore (CA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!