Pbs p4417

The stock solution containing monoclonal antibodies type IgG1 at a concentration of 19.5 mg/mL was provided by Lek Pharmaceuticals d.d. (member of Novartis), and the other reagents were purchased from Sigma (St. Louis, MI, USA): urea (51456), Pluronic F 127 (P2443), HEPES (H3375), BSA (A3294), and PBS (P4417). The mAb particles were prepared by stirring the mAb stock solution on a magnetic stirrer for 2 h @400 RPM at room temperature. The pH of the stock solutions was 7. The isoelectric point of mAb was approximately 8.

PBS (P4417) and BSA (A4503) were from Sigma-Aldrich (St. Louis, MO, USA) and ABTS (37615) was from Thermo Fisher Scientific (Waltham, MA, USA). ELISA kits for interleukin (IL)-1β (88–7261), IL-6 (88–7066), IL-8 (88–8086), IL-10 (88–7106), CCL-2 (88–7399), OSMR (88–50330), IL-1RA (900-M474), BMP-2 (900-TM255), and TIMP-1 (900-M438) were from Thermo Fisher Scientific (Waltham, MA, USA), VEGF (446504) was from BioLegend (San Diego, CA, USA), IL-18 (DY318-05) was from R&D Systems (Minneapolis, MN, USA), and MMP-1 (ab218184) was from Abcam (Cambridge, UK). The Total RNA purification kit was from Norgen Biotek (Thorold, ON, Canada; 17200). The SensiFAST™ cDNA Synthesis Kit (BIO-65054) and SensiFAST™ SYBR^® Lo-ROX Kit (BIO-94050) were purchased from Bioline (London, UK).