Minicollect z serum sep

Six hours post-catecholamine infusion, blood from the rats’ heart were collected into MiniCollect tubes (MiniCollect^® Z Serum Sep, Greiner Bio-One GmbH, Kremsmünster, Austria) and centrifuged at 5878 g for 10 min. Plasma concentrations of N-terminal pro-brain natriuretic peptide (NT-proBNP) and Troponin T were assayed with the Rat NT-proBNP ELISA kit (Cusabio Biotech Co., Ltd., Houston, TX, USA) and Rat cTnT/TNNT2 (Troponin T Type 2, Cardiac) CLIA Kit (#ER1396, Wuhan Fine Biotech Co., Ltd., Wuhan, China).

All included individuals were punctured on finger or heel in order to perform a RDT (CareStart® Malaria, Access Bio Inc, Monmouth Junction, NJ, USA), to perform thick and thin blood smears, and to collect 100–500 μL capillary blood in an appropriate tube (MiniCollect® Z Serum Sep, Greiner Bio-One, Kremsmünster, Austria). Results of RDT were disclosed to individuals or their guardian after the interview, and people with a positive RDT result were proposed to take an anti-malarial treatment according to national guidelines. Thin smears were fixed with absolute methanol and serum tubes were centrifuged 10 min at 2,800 g on a daily basis. Serums tubes were kept at < 22°C until their transfer to the Institut Pasteur de Madagascar, where they were stored at -20°C.